Stimulation of rhesus monkey sperm capacitation by cyclic nucleotide mediators

Boatman, Dorothy E.; Bavister, B. D.

doi:10.1530/jrf.0.0710357

Cited by 140 publications

(72 citation statements)

References 22 publications

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“…Among the several types of media reported, my research group selected TYH medium [103], which was developed for IVF of mouse eggs. Furthermore, by incubation of the spermatozoa in medium that contained caffeine and dibutyryl cyclic AMP, sperm capacitation and hyperactivated motion were induced in spermatozoa of the rhesus monkey (Macaca mulatta) [11], the pig-tailed monkey (Macaca nemestrina) [18], and the cynomolgus monkey [5,56]. Our study also confirmed this finding in cynomolgus monkeys [84], Japanese monkeys [83], and African green monkeys (Cercopithecus aethiops) [78].…”

Section: Sperm Quality In Vivo and In Vitrosupporting

confidence: 83%

In Vitro Manipulation of Nonhuman Primate Gametes for Embryo Production and Embryo Transfer.

Sankai

2000

Exp. Anim.

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Abstract:Since nonhuman primates are closely related to humans and share many physical similarities, they are important for use in research areas such as human infectious diseases, reproduction, physiology, endocrinology, metabolism, neurology and longevity. To develop and maintain these animals, we must establish techniques for in vitro manipulation of spermatozoa and eggs. For a decade my research group has been conducting basic research to establish embryo manipulation techniques and to clarify the reproductive phenomena in nonhuman primates. This article summarizes the past research on in vitro manipulation of nonhuman primate gametes, from collection of reproductive cells and in vitro fertilization to the birth of offspring after embryo transfer, as well as the current status of these research areas. The studies summarized here will directly lead to the development of standard techniques for practical and comprehensive use in nonhuman primates.

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Section: Sperm Quality In Vivo and In Vitrosupporting

confidence: 83%

In Vitro Manipulation of Nonhuman Primate Gametes for Embryo Production and Embryo Transfer.

Sankai

2000

Exp. Anim.

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“…Sperm head-to-head association has also been previously reported to be stimulated by cAMP in the presence of divalent cations such as calcium (Lindahl & Sjö blom 1981, Boatman & Bavister 1984, Harayama et al 1998, Harayama & Kato 2002. In our experiments, evidence for a similar effect in the ram was shown by the increase in non-agglutinated, motile spermatozoa when bicarbonate was omitted from medium (Fig.…”

Section: Discussionmentioning

confidence: 89%

“…Sperm head-to-head association has also been reported in media containing bicarbonate and divalent cations such as calcium (boar (Harayama et al 1998, Harayama & Kato 2002, bull (Lindahl & Sjöblom 1981), monkey (Boatman & Bavister 1984)). The agglutination response was increased by db-cAMP or selective inhibitors of cAMP-specific PDEs indicating this form of agglutination is mediated by a cAMP signalling cascade.…”

Section: Introductionmentioning

confidence: 92%

“…For example, heparin, sheep serum or follicular fluid has been reported to cause head-to-head agglutination of spermatozoa under in vitro conditions in numerous species (monkey (Boatman & Bavister 1984), bull (Ehrenwald et al 1990, Lefebvre & Suarez 1996, pig (Funahashi & Day 1993)). These findings were extended in utero by Brown & Senger (1982) who placed rabbit spermatozoa into the uterine lumen and noted significant agglutination of motile spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

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Penicillamine prevents ram sperm agglutination in media that support capacitation

Leahy¹,

Rickard²,

Aitken³

et al. 2016

Reproduction

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Ram spermatozoa are difficult to capacitate in vitro. Here we describe a further complication, the unreported phenomenon of headto-head agglutination of ram spermatozoa following dilution in the capacitation medium Tyrodes plus albumin, lactate and pyruvate (TALP). Sperm agglutination is immediate, specific and persistent and is not associated with a loss of motility. Agglutination impedes in vitro sperm handling and analysis. So the objectives of this study were to investigate the cause of sperm agglutination and potential agents which may reduce agglutination. The percentage of non-agglutinated, motile spermatozoa increased when bicarbonate was omitted from complete TALP suggesting that bicarbonate ions stimulate the agglutination process. D-penicillamine (PEN), a nucleophilic thiol, was highly effective at reducing agglutination. The inclusion of 250 mM PEN in TALP reduced the incidence of motile, agglutinated spermatozoa from 76.7G2.7% to 2.8G1.4%. It was then assessed if PEN (1 mM) could be included in existing ram sperm capacitation protocols (TALP C1 mM dibutyryl cAMP, caffeine and theophylline) to produce spermatozoa that were simultaneously capacitated and non-agglutinated. This protocol resulted in a sperm population which displayed high levels of tyrosine phosphorylated proteins and lipid disordered membranes (merocyanine-540) while remaining motile, viable, acrosome-intact and non-agglutinated. In summary, PEN (1 mM) can be included in ram sperm capacitation protocols to reduce sperm agglutination and allow for the in vitro assessment of ram sperm capacitation.

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“…Os oócitos foram obtidos por meio de videolaparoscopia após estimulação ovariana controlada, seguindo protocolo adaptado a partir de trabalhos anteriores (ZELINSKI-WOOTEN et al, 1995;OUHIBI et al, 2001 BAVISTER et al, 1984) antes da inseminação.…”

Section: Colheita Avaliação E Preparo Dos Oócitosunclassified

<b>Criopreservação de sêmen de primatas não-humanos</b>

Carvalho¹

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Aos meus irmãos, Mateus e Paulo, pelos conselhos e por sempre "estarem lá" À minha grande família, incluindo tios, tias, primos e primas, pelo apoio incondicional e reuniões descontraídas.Aos meus amigos e amigas que, de longe ou de perto, sempre fizeram a diferença.Ao meu coorientador e amigo, Prof. Rodrigo R. Valle, por ter me incentivado e me ajudado a entrar na pós-graduação; por ter me introduzido à área de À técnica e amiga Cathy Ramsey, do ART Core, que me ajudou durante toda a realização do meu projeto da BEPE.À pesquisadora, Dra. Carol Hanna, que atuou como co-supervisora do meu projeto da BEPE, contribuindo tanto na elaboração como na execução do projeto.Aos demais integrantes do Hennebold Lab, Melinda, Nathan, Jill e Alison, pela ajuda e convivência.À pesquisadora e amiga, Dra. Naida Loskutoff (in memoriam) do Omaha's Henry Doorly Zoo, que também nos deixou precocemente no último ano, pelo fornecimento do corante eosina-fast green. Às instituiçõesAgradeço a todas as instituições que tornaram possível a realização dos meus estudos e pesquisas. No Brasil À Faculdade de Medicina Veterinária e Zootecnia da Universidade de SãoPaulo (FMVZ/USP) por fornecer toda a estrutura técnico-científica para realização do meu Doutorado. Primate Research Center (OHSU) por fornecer toda a estrutura e apoio técnico e financeiro para realização do meu projeto de pesquisa no exterior. Aos AnimaisAgradeço também, aos primatas não-humanos, nossos passivos e inocentes parceiros dessa jornada. better results when compared to vitrification (p<0,05). SW had better results, followed by DGC and SU, while GWF had the worse results. SW and DGC were further evaluated by IVF. Fertilization rates (mean±SEM%) with MI oocytes using fresh sperm were significantly higher (43.5±16.4) than with SW (2.0±2.0) and did not differ from DGC (25.1±14.2). There was no difference in blastocyst rates between treatments (range 0 to 11.9±7.9). Fertilization rates with MII ova were also significantly higher with fresh sperm (41.4±3.6) than DGC and SW (18.4±6.9 and 12.7±7.7, respectively), and more blastocysts developed from MIIs fertilized with fresh sperm (64.7±13.6) than SW and DGC (4.7±4.7 and 30.9±13.8, respectively). In conclusion, frozen-thawed sperm were able to fertilize oocytes and embryos reached the blastocyst stage. DGC yielded better frozen-thawed sperm for IVF in rhesus macaques, when compared with SW.

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Stimulation of rhesus monkey sperm capacitation by cyclic nucleotide mediators

Cited by 140 publications

References 22 publications

In Vitro Manipulation of Nonhuman Primate Gametes for Embryo Production and Embryo Transfer.

In Vitro Manipulation of Nonhuman Primate Gametes for Embryo Production and Embryo Transfer.

Penicillamine prevents ram sperm agglutination in media that support capacitation

<b>Criopreservação de sêmen de primatas não-humanos</b>

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