Stimulation of Sessile Growth of A Biofilm-Deficient Strain<i>Escherichia Coli</i>K-12 by Protein(s) Secreted by Enterobacteriaceae Species

Vacheva, Anna; Ivanova, Radostina; Stoitsova, Stoyanka

doi:10.5504/bbeq.2011.0110

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…Synergistic interactions of this type have earlier been described for both laboratory strains in mixed biofilm experiments [40], and biofilms formed by natural consortia [41][42][43]. The stimulation of sessile growth of biofilm deficient strains could be accomplished by both cell-to-cell interactions [44] and released products of the adherence-proficient strains [45,46]. According to Pathak [4] the microbial heterogeneity of the biofilm matrix promotes fulfilling of nutrient requirements and poses in the close vicinity the substrate and the microorganisms.…”

Section: Discussionmentioning

confidence: 98%

Degradation of Poly(ε-caprolactone) by a Thermophilic Community and Brevibacillus thermoruber Strain 7 Isolated from Bulgarian Hot Spring

et al. 2021

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The continual plastic accumulation in the environment and the hazardous consequences determine the interest in thermophiles as possible effective plastic degraders, due to their unique metabolic mechanisms and change of plastic properties at elevated temperatures. PCL is one of major biodegradable plastics with promising application to replace existing non-biodegradable polymers. Metagenomic analysis of the phylogenetic diversity in plastic contaminated area of Marikostinovo hot spring, Bulgaria revealed a higher number taxonomic groups (11) in the sample enriched without plastic (Marikostinovo community, control sample, MKC-C) than in that enriched in the presence of poly-ε-caprolactone (PCL) (MKC-P), (7). A strong domination of the phylum Proteobacteria was observed for MKC-C, while the dominant phyla in MKC-P were Deinococcus-Thermus and Firmicutes. Among the strains isolated from MKC-P, the highest esterase activity was registered for Brevibacillus thermoruber strain 7 at 55 °C. Its co-cultivation with another isolate resulted in ~10% increase in enzyme activity. During a 28-day biodegradation process, a decrease in PCL molecular weight and weight loss were established resulting in 100% degradation by MKC-P and 63.6% by strain 7. PCL degradation intermediate profiles for MKC-P and pure strain were similar. Broken plastic pieces from PCL surface and formation of a biofilm by MKC-P were observed by SEM, while the pure strain caused significant deformation of PCL probes without biofilm formation.

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