Stimulation of Streptomycin-Resistant Bacteria in the Rhizosphere of Leguminous Plants

Brown, Michael

doi:10.1099/00221287-24-3-369

Cited by 21 publications

(3 citation statements)

References 6 publications

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“…The enrichment of antibiotic (streptomycin) resistant bacteria in the rhizosphere and rhizoplane of some plants has become increasingly accepted (Baldani etal., 1982;Brown, 1961;Dtbereiner and Baldani., 1979;Lopes-Reyes et al, 1989). The enrichment of antibiotic (streptomycin) resistant bacteria in the rhizosphere and rhizoplane of some plants has become increasingly accepted (Baldani etal., 1982;Brown, 1961;Dtbereiner and Baldani., 1979;Lopes-Reyes et al, 1989).…”

Section: Discussionmentioning

confidence: 99%

Effects of antibiotics in soil on the population dynamics of transposon Tn5 carrying Pseudomonas fluorescens

Oliveira¹,

Wolters²,

Elsas³

1995

Plant Soil

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The effects of kanamycin and streptomycin added to soil on the survival of transposon Tn5 modified Pseudomonas fluorescens strain R2f were investigated. Kanamycin in high (180/.tg g-1 dry soil) or low (18/_tg g-1 ) concentration or streptomycin in low concentration in Ede loamy sand soil had no noticeable effect on inoculant population dynamics in soil and wheat rhizosphere, whereas streptomycin in high concentration had a consistent significant stimulatory effect, in particular in the wheat rhizosphere. Streptomycin exerted its effect by selecting P fluorescens with Tn5 insertion whilst suppressing the unmodified sensitive parent strain, as evidenced by comparing the behaviour of these two strains in separate and mixed inoculation studies.Soil textural type influenced the effect of streptomycin on the Tn5 carrying inoculant; the effect was consistently detected in rhizosphere and rhizoplane samples of wheat grown in Ede loamy sand after 7 and 14 days incubation, whereas it was only apparent after 7 days in rhizoplane or rhizosphere (and bulk soil) samples of wheat grown in two silt loam soils. Modification of soil pH by the addition of CaCO3 or bentonite clay resulted in an enhancement of the selective effect of streptomycin by CaCO3 and its abolishment by bentonite clay.The addition to soil of malic acid or wheat root exudate, but not of glucose, enhanced the streptomycin selective effect on the Tn5-modified P fluorescens strain. Neither the streptomycin producer Streptomyces griseus nor two non-inhibiting mutants obtained following UV irradiation affected the dynamics of P fluorescens (chr::Tn5) in soil and wheat rhizosphere.The effect of streptomycin in soil on inoculant Tn5 carrying bacteria depends on conditions such as soil type, the presence of (wheat) root exudates and the type of available substrate.

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Section: Discussionmentioning

confidence: 99%

Effects of antibiotics in soil on the population dynamics of transposon Tn5 carrying Pseudomonas fluorescens

Oliveira¹,

Wolters²,

Elsas³

1995

Plant Soil

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“…Additionally, in the noPhCs-P columns, the sul 1–2 and int 1 genes were also detected in higher number of replicates and for a longer period compared to the noPhCs-U columns, despite the lack of antimicrobial agents in the feed. It was suggested, e.g., by Brown (1961) or by Harris and Woodbine (1967), that the survival of rhizospheric bacteria can be also associated with the enhanced resistance to several environment stressors. The potential ecological significance of this phenomenon was, however, under dispute, since studies mentioned above were based on the culture-dependent techniques.…”

Section: Resultsmentioning

confidence: 99%

The treatment of wastewater containing pharmaceuticals in microcosm constructed wetlands: the occurrence of integrons (int1–2) and associated resistance genes (sul1–3, qacEΔ1)

Nowrotek

Kotlarska

Łuczkiewicz

et al. 2017

Environ Sci Pollut Res

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The aim of this study was to analyze the occurrence of sulfonamide resistance genes (sul1–3) and other genetic elements as antiseptic resistance gene (qacEΔ1) and class 1 and class 2 integrons (int1–2) in the upper layer of substrate and in the effluent of microcosm constructed wetlands (CWs) treating artificial wastewater containing diclofenac and sulfamethoxazole (SMX), which is a sulfonamide antibiotic. The bacteria in the substrate and in the effluents were equipped with the sul1–2, int1, and qacEΔ1 resistance determinants, which were introduced into the CW system during inoculation with activated sludge and with the soil attached to the rhizosphere of potted seedlings of Phalaris arundinacea ‘Picta’ roots (int1). By comparing the occurrence of the resistance determinants in the upper substrate layer and the effluent, it can be stated that they neither were lost nor emerged along the flow path. The implications of the presence of antibiotic resistance genes in the effluent may entail a risk of antibiotic resistance being spread in the receiving environment. Additionally, transformation products of SMX were determined. According to the obtained results, four (potential) SMX transformation products were identified. Two major metabolites of SMX, 2,3,5-trihydroxy-SMX and 3,5-dihydroxy-SMX, indicated that SMX may be partly oxidized during the treatment. The remaining two SMX transformation products (hydroxy-glutathionyl-SMX and glutathionyl-SMX) are conjugates with glutathione, which suggests the ability of CW bacterial community to degrade SMX and resist antimicrobial stress.Electronic supplementary materialThe online version of this article (doi:10.1007/s11356-017-9079-1) contains supplementary material, which is available to authorized users.

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“…Ten-fold serial dilutions were plated in order to count bacteria, fungi and actinomycetes according to Brown (1961), Martin (1950) and Pridham et al (1956), respectively. 02 consumption was assayed at 28°C on 3 ml of the suspension by an oxygen monitor YSI mod 240/B.…”

Section: Methodsmentioning

confidence: 99%

Olive-Mill Wastewater Composting: Microbiological Aspects

Galli¹,

Pasetti²,

Fiorelli³

et al. 1997

Waste Manag Res

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Oxygen consumption, microbial growth, lignin and phenol degradation, urease, protease and nitrogenase activity were determined during the composting of an olivemill wastewaters (OMW)—wheat straw mixture. Oxygen consumption, microbial growth and urease activity were greatly enhanced during the thermophilic phase, reaching their maximum in about three weeks. Casein-hydrolysing protease showed a high initial activity which sharply decreased after 2 weeks. At the end of the thermophilic phase both phenols and lignin were reduced by about 70%. Composting enhanced diazotrophic microflora as indicated by nitrogenase activity which increased at the end of the thermophilic phase. © 1997 ISWA

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Stimulation of Streptomycin-Resistant Bacteria in the Rhizosphere of Leguminous Plants

Cited by 21 publications

References 6 publications

Effects of antibiotics in soil on the population dynamics of transposon Tn5 carrying Pseudomonas fluorescens

Effects of antibiotics in soil on the population dynamics of transposon Tn5 carrying Pseudomonas fluorescens

The treatment of wastewater containing pharmaceuticals in microcosm constructed wetlands: the occurrence of integrons (int1–2) and associated resistance genes (sul1–3, qacEΔ1)

Olive-Mill Wastewater Composting: Microbiological Aspects

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