Stimulation of the respiratory burst and promotion of bacterial killing in human granulocytes by intravenous immunoglobulin preparations

Maródi, László; Kalmár, A; Karmazsin, L

doi:10.1111/j.1365-2249.1990.tb05173.x

Cited by 10 publications

(7 citation statements)

References 31 publications

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“…However, high-dose IVIG, 2 g/kg, is generally required for IVIG's anti-inflammatory effects, whereas protection in the rabbit model of necrotizing pneumonia was achieved using 1 / 10 of the human dose, 200 mg/kg. If there was an anti-inflammatory effect in the rabbit model, then it was likely to be minimal because 59 of 64 (92%) rabbits administered IVIG (200 mg The role of IVIG in enhancing in vitro opsonophagocytic killing of S. aureus has been debated (29)(30)(31)(32)(33). Notably, rabbits administered IVIG depleted of its antitoxin antibodies did not differ in lung bacterial counts from those treated with saline ( Fig.…”

Section: Discussionmentioning

confidence: 99%

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IVIG-mediated protection against necrotizing pneumonia caused by MRSA

Diep

Badiou

et al. 2016

Sci. Transl. Med.

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New therapeutic approaches are urgently needed to improve survival outcomes for patients with necrotizing pneumonia caused by Staphylococcus aureus One such approach is adjunctive treatment with intravenous immunoglobulin (IVIG), but clinical practice guidelines offer conflicting recommendations. In a preclinical rabbit model, prophylaxis with IVIG conferred protection against necrotizing pneumonia caused by five different epidemic strains of community-associated methicillin-resistant S. aureus (MRSA) as well as a widespread strain of hospital-associated MRSA. Treatment with IVIG, either alone or in combination with vancomycin or linezolid, improved survival outcomes in this rabbit model. Two specific IVIG antibodies that neutralized the toxic effects of α-hemolysin (Hla) and Panton-Valentine leukocidin (PVL) conferred protection against necrotizing pneumonia in the rabbit model. This mechanism of action of IVIG was uncovered by analyzing loss-of-function mutant bacterial strains containing deletions in 17 genes encoding staphylococcal exotoxins, which revealed only Hla and PVL as having an impact on necrotizing pneumonia. These results demonstrate the potential clinical utility of IVIG in the treatment of severe pneumonia induced by S. aureus.

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Section: Discussionmentioning

confidence: 99%

“…The role of IVIG in enhancing in vitro opsonophagocytic killing of S. aureus has been debated (29)(30)(31)(32)(33). Notably, rabbits administered IVIG depleted of its antitoxin antibodies did not differ in lung bacterial counts from those treated with saline (Fig.…”

Section: Discussionmentioning

confidence: 99%

IVIG-mediated protection against necrotizing pneumonia caused by MRSA

Diep

Badiou

et al. 2016

Sci. Transl. Med.

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“…These locally large amounts of Igs may reduce the deleterious effect of local degradation established by, for instance, proteases from granulocytes and bacteria (19). Furthermore, this implies enhanced ef cacy of the bacterial elimination as high concentrations of Igs increase both the capacity of the phagocytic cell to engulf the bacteria and its bactericidal function (18,20). In addition, the Igs in the surface secretion may have an in uence on the T-lymphocytes invariably present in the surface secretion (10).…”

Section: Discussionmentioning

confidence: 98%

Phagocytosis in the Nasopharyngeal Secretion by Cells from the Adenoid

Ivarsson

Lundberg²

2001

Acta Oto-Laryngologica

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The aim of this study was to elucidate whether granulocytes and macrophages in surface secretion on the adenoid emanate from the adenoid and whether these cells participate in the control of the nasopharyngeal bacterial flora. Samples of the adenoid and its surface secretion were obtained during adenoidectomy from 12 children with recurrent acute otitis media, secretory otitis media or enlarged adenoids causing obstruction. Immunochemistry was used to examine the location of granulocytes and macrophages in the adenoid as well as the presence of IgA, IgM, IgG and plasma cells in the secretion. Phagocytosis in the secretion was examined in imprints stained with May Grünwald Giemsa. Acridine Orange and Gram staining were used to demonstrate the presence and location of bacteria in the secretion and mucosa. As a control, surface secretions were obtained from 12 children without any history of recurrent airway problems. Granulocytes and macrophages were observed in the epithelium of the adenoid and some of these cells penetrated the epithelial surface. Positive staining for IgA, IgM and IgG was observed in all secretions. In 10 of 12 children plasma cells were present in the secretion. Bacteria were observed in all imprints. With the exception of I child in each group phagocytosis of bacteria in the surface secretion was demonstrated from imprints in all children. We conclude that granulocytes and macrophages leave the adenoid and enter the surface secretion, where constant phagocytic activity takes place. The spatial relations between mononuclear and polymorphonuclear cells imply a possible cooperation between these cells in the overall control of the nasopharyngeal bacterial flora.

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“…Therefore, NADPH oxidase‐derived ROS may be included in the mechanism for IVIG‐induced apoptosis in LPS‐stimulated neutrophils. IVIG has been reported to increase the production of superoxide anion, promote bacterial killing (40), and induce degranulation (41). As a result, IVIG‐induced neutrophil apoptosis is simultaneously accompanied by their functional activation and the production of ROS and bioactive enzymes.…”

Section: Discussionmentioning

confidence: 99%

Intravenous immunoglobulin preparations promote apoptosis in lipopolysaccharide‐stimulated neutrophils via an oxygen‐dependent pathway in vitro

Takeshita

Nakatani

2005

APMIS

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Since prolonged survival of activated neutrophils has an autotoxic potential, neutrophil apoptosis plays an important role in the rapid resolution of inflammation. Intravenous immunoglobulin (IVIG) preparations, which are beneficial therapeutic agents for the treatment of autoimmune diseases and systemic inflammatory diseases, have been reported to induce apoptosis of lymphocytes and endothelial cells in vitro. In the present study, we investigated whether IVIG may induce apoptosis of neutrophils cultured in vitro. After neutrophils prestimulated with or without lipopolysaccharide (LPS) were cultured in the presence or absence of IVIG, the number of apoptotic cells, intracellular H2O2 and GSH were measured by a flow cytometer. IVIG induced apoptosis of LPS-stimulated neutrophils dose dependently, but not in unstimulated neutrophils. Although anti-Fas monoclonal antibodies (mAbs) had no effect on the IVIG-induced apoptosis in the LPS-stimulated neutrophils, anti-Fc gamma receptor (Fc gammaR) II- and III-blocking mAbs significantly inhibited the IVIG-induced apoptosis. IVIG increased the production of intracellular H2O2, while it decreased the production of GSH, in the LPS-stimulated neutrophils. Furthermore, a specific NADPH oxidase inhibitor and anti-oxidants inhibited the IVIG-induced neutrophil apoptosis. Therefore, these findings indicate that IVIG preparations induce apoptosis of LPS-stimulated neutrophils and suggest that the IVIG-induced apoptosis may be mediated by an oxygen-dependent pathway via Fc gammaRII and III.

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Stimulation of the respiratory burst and promotion of bacterial killing in human granulocytes by intravenous immunoglobulin preparations

Cited by 10 publications

References 31 publications

IVIG-mediated protection against necrotizing pneumonia caused by MRSA

IVIG-mediated protection against necrotizing pneumonia caused by MRSA

Phagocytosis in the Nasopharyngeal Secretion by Cells from the Adenoid

Intravenous immunoglobulin preparations promote apoptosis in lipopolysaccharide‐stimulated neutrophils via an oxygen‐dependent pathway in vitro

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