Stimulation of Tumor-Specific Immunity by p5 HER-2/neu Generated Peptide Encapsulated in Nano-liposomes with High Phase Transition Temperature Phospholipids

Abstract

The results indicated that simple liposomes consisting of phospholipids with high transition temperature could be an effective vaccine vehicle for tumor-associated antigens for inducing cell mediate immunity.

Preparation of nanoliposomes linked to HER2/neu‐derived (P5) peptide containing MPL adjuvant as vaccine against breast cancer

“…2,27 It was also reported that the formulation of P5 peptide encapsulated in DSPC:DSPG nanoliposomes could significantly induce IFN-γ secretion and antigen-specific CTL responses, which led to a lower tumor size and a longer survival time when compared with the control group. 21 Similarly, our results also showed that P5-peptideconjugated liposomal formulation composed of DSPC, DSPG, Chol, and DOPE (Tm: 54°C) containing MPL could efficiently stimulate CTL responses against TUBO tumor cells. According to a previous study, immunogenicity of P5 peptide was enhanced via conjugation of P5 on the surface of liposome nanoparticles (DMPC; DMPG; Chol).…”

“…DSPC‐contained liposome formulations have been found to be significantly effective in stimulating Th1 and CTL responses to both entrapped and membrane‐linked antigens . It was also reported that the formulation of P5 peptide encapsulated in DSPC:DSPG nanoliposomes could significantly induce IFN‐γ secretion and antigen‐specific CTL responses, which led to a lower tumor size and a longer survival time when compared with the control group …”

Nanoparticle drug delivery systems: an excellent carrier for tumor peptide vaccines

“…On the basis of Mansourian, making use of chaotropic transfer technique, new researches from Yazdani et al. ( 2017 ) made liposomes with high transition temperature (T m ) to sustain the stability, in the function of a vaccine for P5 HER2/neu generated peptide. After evaluation, especially in experiments in vivo the results suggested that immunization with Lip-p5 nanoliposomes has enhanced the antigen-specific IFN-γ response of CD8 + T cells and induced CTL response in comparison with free peptides and empty liposomes, which resulted in a smaller tumor and longer survival time.…”

P435 HER2/neu-derived peptide conjugated to liposomes containing DOPE as an effective prophylactic vaccine formulation for breast cancer

“…Increasing cellular and humoral response against HER2/neu has been associated with decreased tumor development and improved prognosis [24]. In our previous study, several HER2/neu epitopes were formulated in different nanoliposomes, which could induce effective immune responses against HER2-positive murine tumor model [25][26][27][28]. Our findings demonstrated that P435, as a short HER2 epitope, was an effective peptide which inducing CTL responses and improving prognosis of cancer in TUBO mice model of breast cancer [29].…”