Stimulatory and Inhibitory Differentiation of Human Myeloid Dendritic Cells

Chakraborty, Abalokita; Li, Li; Chakraborty, Nitya G.; Mukherji, Bijay

doi:10.1006/clim.1999.4826

Cited by 27 publications

(24 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4A shows that the siRNA treatment had no inhibitory effect on the DC surface expression of co-stimulatory molecules. In fact, in agreement with previous reports from other groups as well as our laboratory that IL-10 negatively affects expression of co-stimulatory molecules on DC [5][6][7][8], IL-10 knocked down DC had slightly higher levels of co-stimulatory molecules expression (Fig. 4A).…”

Section: Silencing Of Il-10 In Dendritic Cells Results In Increased Isupporting

confidence: 93%

“…Utilizing gene micro-array analysis of IL-10 treated DC we confirmed our initial observations and showed that IL-10 treatment does indeed downregulates the gene expression of IL-12 and co-stimulatory molecules in human DC [25]. Our findings have also been independently confirmed by other groups [7] and it has been shown that exogenous IL-10 treatment of monocytes results in down-regulated expression of costimulatory molecules [5][6][7] and such treatment can affect the generation of Th1 type of immune responses [10].…”

Section: Discussionsupporting

confidence: 84%

“…We have previously shown that even fully matured DC can synthesize IL-10 and such IL-10 producing DC can suppress T cell proliferation [5,6]. Utilizing gene micro-array analysis of IL-10 treated DC we confirmed our initial observations and showed that IL-10 treatment does indeed downregulates the gene expression of IL-12 and co-stimulatory molecules in human DC [25].…”

Section: Discussionsupporting

confidence: 75%

“…It has been shown that the functional phenotype of DC can be influenced by interfering with the expression of important DC-tropic cytokine genes such as IL-10 and IL-12 [5,6]. We have previously shown that even fully matured DC can synthesize IL-10 and such IL-10 producing DC can suppress T cell proliferation [5,6].…”

Section: Discussionmentioning

confidence: 99%

“…For example, Th1 type T cell responses need IL-12 synthesis by DC. DC can also produce immunosuppressive cytokines, such as IL-10 [5][6][7], which can influence the nature and the quality of the T cell response. IL-10 produced by DC can influence the DC maturation process, down-regulate IL-12 production and thus interfere with the Th1 type T cell response generation [3,[8][9][10].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Silencing of endogenous IL-10 in human dendritic cells leads to the generation of an improved CTL response against human melanoma associated antigenic epitope, MART-127–35

Chhabra

Chakraborty

Mukherji

2008

Clinical Immunology

Self Cite

View full text Add to dashboard Cite

Dendritic cells (DC) present antigenic epitopes to and activate T cells. They also polarize the ensuing T cell response to Th1 or Th2 type response, depending on their cytokine production profile. For example, IL-12 producing DC generate Th1 type T cell response whereas IL-10 producing DC is usually tolerogenic. Different strategies --such as the use of cytokines and anti-cytokine antibodies, dominant negative forms of protein, anti-sense RNA etc. --have been employed to influence the cytokine synthetic profile of DC as well as to make DC more immunogenic. Utilizing GFP expressing recombinant adenoviruses in association with lipid-mediated transfection of siRNA, we have silenced the endogenous IL-10 gene in DC. We show that IL-10 gene silenced DC produce more IL-12 and also generates a better cytolytic T cell response against the human melanoma associated epitope, MART-1 27−35 , in-vitro. We also show that the GFP expressing adenoviral vector can be used to optimize the parameters for siRNA delivery in primary cells and show that RNA interference methodology can efficiently knock-down virus encoded genes transcribed at very high multiplicity of infection in DC.

show abstract

Section: Silencing Of Il-10 In Dendritic Cells Results In Increased Isupporting

confidence: 93%