Stimulatory effect of pancreozymin-cholecystokinin on calcium secretion in pancreatic juice of dogs

Goebell, H.; Steffen, Ch.; Bode, Ch.

doi:10.1136/gut.13.6.477

Cited by 34 publications

(23 citation statements)

References 13 publications

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“…controls, Figure 2 (Vallee, Stein, Sumerwell & Fisher, 1959). Ca++ is secreted into pancreatic juice in direct relation to the enzyme following stimulation with pancreozymin (Zimmerman, Dreiling, Rosenberg & Janowitz, 1967;Goebell, Steffen & Bode, 1972). That the 45Ca effluxed from the pancreas in the current experiments is not complexed to the o-amylase secreted, is suggested by a number of observations.…”

Section: Effect Of Atropinementioning

confidence: 62%

Calcium Efflux and Secretion of Α‐amylase From Rat Pancreas

Heisler

1974

British J Pharmacology

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1 The efflux of 45Ca from rat pancreas was investigated in relation to the secretion of c-amylase.2 The 45Ca space in the pancreas was increased 1.6 times when pancreatic tissue was incubated in media in which the extracellular calcium concentration was reduced from 1.0 to 0.05 mM.3 Carbachol increased the rate of 45Ca efflux from the tissue and this effect was associated with an increase in the release of a-amylase. Dibutyryl cyclic adenosine monophosphate (AMP) also evoked a secretory response, but did not alter the rate of 45Ca efflux. In combination with carbachol, the dibutyryl analogue of cyclic AMP reduced the carbachol-stimulated increase in 45Ca efflux while enhancing the release of secretory protein. 4 The stimulatory effect of carbachol on 45Ca efflux was observed when pancreatic tissues were incubated in media containing a high concentration of ethyleneglycolbis (,B-aminoethyl)-N,NA-tetraacetic acid (EGTA). 5Atropine blocked the effects of carbachol on both 4Ca efflux and secretion of ci-amylase. 6 It was concluded that intracellular calcium can and may sustain stimulus-secretion coupling in the rat exocrine pancreas.

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Section: Effect Of Atropinementioning

confidence: 62%

Calcium Efflux and Secretion of Α‐amylase From Rat Pancreas

Heisler

1974

British J Pharmacology

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“…This second calcium component must arise either by way of the electrolyte secretary mechanism or by diffusion through the duct system. A similar two-component hypothesis for pancreatic calcium secretion has been formulated independently by Goebell et al (1972). Effect of calcium and other cations on enzyme secretion.…”

Section: Discussionmentioning

confidence: 92%

Amylase secretion by the perfused cat pancreas in relation to the secretion of calcium and other electrolytes and as influenced by the extrenal ionic environment*

Argent¹,

Case²,

Scratcherd³

1973

The Journal of Physiology

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“…Previous studies carried out in intact animals as well as in in vitro preparations (Goebel, Steffen & Bode, 1972; Argent, Case & Scratcherd, 1973) suggest that calcium reaches the pancreatic juice from at least two different sources. One of these sources appears to be correlated with, and the other independent of the secretary function of the acinar cells of the gland.…”

Section: Introductionmentioning

confidence: 99%

Secretion of calcium in pancreatic juice.

Ceccarelli¹,

Clémente²,

Meldolesi

1975

The Journal of Physiology

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SUMMARY1. The origin of the calcium secreted by the pancreas has been investigated in vivo in the guinea-pig by a study carried out in parallel (a) in the juice secreted in response to the injection of either secretin or caerulein and (b) in the pancreatic tissue and in cell fractions isolated therefrom.2. In agreement with previous findings we observed that the concentration of calcium is low in the secretin-stimulated and high in the caeruleinstimulated juice. In the latter calcium and protein are proportional (ca. 50 n-mole: mg).3. After i.v. injection of 45Ca the radioactivity decreases rapidly and quasi-exponentially in the blood plasma. A roughly parallel time course is found in the secretin-stimulated juice: the evolution of the juice: plasma radioactivity ratio resembles that observed with the extracellular space marker [3H]D-sorbitol. In contrast, the time course of 45Ca in plasma and caerulein-stimulated juice are not proportional: the high levels characteristic of this juice are reached several minutes after the injection and maintained thereafter. This increase is followed ca. 50 min later by the appearance of the newly synthesized [3H]L-leucine-labelled proteins.4. The pancreatic tissue is rich in calcium which is localized primarily in zymogen granules (ca. 36 n-mole: mg protein) and mitochondria; the soluble cytoplasm is low in calcium.5. The injected "Ca accumulates in zymogen granules faster than [3H]L-leucine-labelled proteins. The 45Ca : protein ratio of these organelles is considerably lower than that of the caerulein-stimulated juice.6. It is concluded (a) that calcium is secreted into the pancreatic juice in two fractions, one (possibly released by simple diffusion) associated with the electrolyte component, the other with the protein of the juice,

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Stimulatory effect of pancreozymin-cholecystokinin on calcium secretion in pancreatic juice of dogs

Cited by 34 publications

References 13 publications

Calcium Efflux and Secretion of Α‐amylase From Rat Pancreas

Calcium Efflux and Secretion of Α‐amylase From Rat Pancreas

Amylase secretion by the perfused cat pancreas in relation to the secretion of calcium and other electrolytes and as influenced by the extrenal ionic environment*

Secretion of calcium in pancreatic juice.

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