Stimulatory Effects of LH, FSH and Prostaglandins upon Cyclic 3′,5′-AMP Levels in Porcine Granulosa Cells1

The Sheep Ovary: Regulation of Steroidogenic, Haemodynamic and Structural Changes in the Largest Follicle and Adjacent Tissue Before Ovulation

“…We have tested some aspects of this hypothesis using the ovine follicle in culture as a model (R. M. Moor & R. F. Seamark, unpublished observations). Incubation with indomethacin, aspirin or flufenamic acid, agents which block prostaglandin synthesis (see Sanner, 1974) Kolena & Channing, 1972;Lindner et al, 1974) and suggest that prostaglandins are unlikely to be obligatory intrafollicular mediators of LH. Effect of LH on the enzyme system responsible for aromatization Indirect evidence obtained by determining the rate of substrate accumulation in preovulatory follicles has indicated that the aromatase and then the desmolase system limit steroid production in the theca interna before ovulation (Seamark et al, 1974 …”

Effects of puberty and gonadotropins on the molecular events controlling meiotic resumption of mouse oocytes

“…Oocytes remain in a GV state as long as the MPF is inactive, and the activation of MPF triggers GVBD. Gonadotropins interact with their receptors on the membrane of granulosa cells or oocytes to activate adenylate cyclase (ADCY; Kolena & Channing 1972, Hunzicker-Dunn & Birnbaumer 1976, Olsiewski & Beers 1983. The hormone-receptor binding causes a conformation change in the receptor, allowing its interaction with the G protein, and thus, the hormonal signal is transduced from the receptor to ADCY to catalyze the transformation of ATP into cAMP (Gilman 1990).…”

Hormonal regulation of preovulatory follicle maturation in the rat

“…2) was inhibited in the presence of the non-steroidal antiandrogen, SCH-16423 ), a derivative of flutamide which inhibits ovarian nuclear uptake of tritiated testosterone in vivo (Zeleznik, Hillier & Ross, 1979), presumably by inhibiting the nuclear translocation of cytoplasmic testosterone-receptor complexes within granulosa cells (Schreiber & Ross, 1976). Androgen has also been shown to stimulate progestagen production by cultured granulosa cells from mature antral follicles of cyclic rats and pigs (Nimrod & Lindner, 1976; Gonadotrophin-induced steroidogenesis in follicular cells is mediated by increases in the intracellular concentration of 3',5'-cAMP which is thought to initiate a complex sequence of subcellular biochemical events which culminate in the induction or activation of one or more of the rate-limiting enzymic steps in steroidogenesis (Kolena & Channing, 1972;Marsh, 1976 Fig. 2) led to readily detectable basal secretion of progesterone (no additions to culture medium) which was stimulated 3-4-and 1-6-fold after 240 min in the presence of FSH and dibutyryl cAMP, respectively.…”