Stimulatory effects of sea salts on cell growth in liquid culture of Avicenniaceae mangrove

Hayashi, Shinji; Kuriyama, Shingo; Kawana, Yoshifumi; Hasegawa, Akira; Kurita, Asami; Minagawa, Reiko; Sasamoto, Hamako

doi:10.5511/plantbiotechnology.26.561

Cited by 12 publications

(18 citation statements)

References 12 publications

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“…These findings are consistent with the fact that addition of 10 mM CaCl 2 inhibited the growth of cotyledon-origin suspension cells (Hayashi et al, 2009), when the packed cell volume was measured after culture of suspension cells using a 24-well culture plate. In the present study, growth of callus was measured as increased pixel area using Image-J software.…”

Section: Cacl 2 Inhibition On the Growth Of High-ca Callus Line And Tsupporting

confidence: 78%

“…These ions are constituent ions in seawater and soil and the habitat of mangrove plants contain various amounts of each ion (Dagar, Singh, & Mongia, 1993). A. alba suspension cells showed tolerance or halophilism to Na + , K + , Mg 2+ , Cl -, and SO 4 2-ions; however, further addition of 10 mM of CaCl 2 , was inhibitory to growth (Hayashi et al, 2009), while S. alba suspension cells showed halophilic nature to all of the salts investigated including Ca 2+ . The protoplast cultures showed similar differences in Ca 2+ effect between S. alba and A. alba .…”

Section: Introductionmentioning

confidence: 99%

“…The latter two Sonneratia species are less tolerant to Na + salt . By contrast, in Avicennia mangrove species, only A. alba could be sub-cultured as a suspension culture in the modified amino acid (mAA) basal medium (Hayashi et al, 2009), in which the concentration of CaCl 2 (3 mM) is the same as the MS basal medium. Though the halophilic nature to Na + and Mg 2+ was shown in leaf culture of another seaward side grown mangrove species, A. marina (Hayashi et al, 2009), callus growth in sub-culture has not been established in Avicennia species other than A. alba.…”

Section: Introductionmentioning

confidence: 99%

“…We investigated the halophilic and salts-tolerant nature using cotyledon-derived suspension cultures of S. alba and A. alba (Hayashi et al, 2009) and their protoplast cultures (Hasegawa, Kurita, Hayashi, Fukumoto, & Sasamoto, 2013). The effects of adding high concentrations (10-200 mM) of Na + , K + , Mg 2+ , Ca 2+ , Cl -, and SO 4 2-ions in the medium, on the growth of suspension cells and protoplasts were investigated.…”

Section: Introductionmentioning

confidence: 99%

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Cellular Calcium Distribution Modulates the Growth of Callus and Protoplasts of Halophyte Mangrove Plant, Avicennia Alba - an X-ray Microanalysis

Hayatsu¹,

Suzuki²,

Tsuchiya³

et al. 2017

JPS

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Two cultured cell lines were developed from cotyledons of a halophyte mangrove, Avicennia alba. In the high-Ca callus line, which was sub-cultured in a modified amino acid medium containing 3 mM CaCl 2 , growth of calluses and their protoplasts were both inhibited by low concentrations of CaCl 2 in the culture medium. Removal of Ca 2+ from the culture medium stimulated callus growth and the calluses could be sub-cultured without CaCl 2 (low-Ca callus line). The intra-(cytoplasmic matrix and vacuole) and extra-(cell wall) cellular concentrations of elements, i.e., [Ca] [S] were investigated using quantitative X-ray microanalysis of cryosections of calluses from both cell lines. [Ca] was high in the cytoplasmic matrix and cell wall of the high-Ca line. [Ca] was lowered in the low-Ca line in all cell compartments, though still detected. Ca-containing electron-dense precipitates were accumulated in the middle lamella of cell walls in resin-embedded sections of the high-Ca line. CaCl 2 in the medium stimulated protoplast growth only in the low-Ca line. These results suggested that a low cellular [Ca] is needed for protoplasts growth of A. alba. The importance of cellular [Ca] for the growth of halophilic mangrove plant cells was discussed.

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Section: Cacl 2 Inhibition On the Growth Of High-ca Callus Line And Tsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cellular Calcium Distribution Modulates the Growth of Callus and Protoplasts of Halophyte Mangrove Plant, Avicennia Alba - an X-ray Microanalysis

Hayatsu¹,

Suzuki²,

Tsuchiya³

et al. 2017

JPS

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“…Strong salt tolerance at the cellular level of several mangrove plants, growing in the coastal region with a high salinity, has been found using tissue cultured cells and protoplasts (Kawana & Sasamoto, 2008;Hayashi et al, 2009;Yamamoto et al, 2011;Hasegawa et al, 2013).…”

Section: Salt Tolerance and Allelopathy Of Mangrove Cultured Cellsmentioning

confidence: 99%

Evaluation of an Anthocyanin, Cyanidin 3,5-di-O-glucoside, as an Allelochemical in Red Callus of a Mangrove Sonneratia ovata, Using Protoplast Co-Culture Bioassay Method with Digital Image Analysis

Sasamoto

Iwashina

Suzuki

et al. 2018

JPS

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Protoplasts isolated from red callus of a mangrove, Sonneratia ovata were co-cultured with recipient lettuce protoplasts to examine the allelopathic activities. Protoplasts were isolated with Cellulase R10 and Driselase 20 in 0.6 M mannitol solution and purified by density gradient centrifugation on 0.6 M sucrose. Protoplasts were co-cultured in 50 μL of liquid Murashige and Skoog's (MS) basal medium containing 1 μM 2,4-dichlorophenoxyacetic acid and 0.1 μM benzyladenine and 0.6 M mannitol solution in a 96-well culture plate. Protoplast density ranged from 5 × 10 3 /mL to 10 5 /mL. Cell division of lettuce protoplasts was strongly inhibited by addition of S. ovata protoplasts, and non-spherical cell enlargement was slightly inhibited. By contrast, digital image analysis of scanned 96-well culture plates revealed no inhibition in accumulation of yellow color in lettuce protoplasts. An anthocyanin, cyanidin 3,5-di-O-glucoside (cyanin), was identified and its content in the red callus was ca. 1 mM of fresh weight. The effects of cyanin on the growth of lettuce protoplasts at three stages were similar to those of red S. ovata protoplasts. From these results, cyanin was most likely the allelochemical contained in red callus of S. ovata. The allelopathic activity of cyanin was compared with that of other putative allelochemicals in several plant materials, using the protoplast co-culture method with digital image analysis.

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Mangroves: obligate or facultative halophytes? A review

Wang

Yan

You

et al. 2011

Trees

101

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Salinity plays significant roles in regulating the growth and distribution of mangroves, and the salt tolerance mechanisms of mangroves have been the focus of research for several decades. There are contradictory views regarding the relationship between mangroves and salt: (1) Mangroves are facultative halophytes, i.e. freshwater is a physiological requirement and salt water is an ecological requirement for mangroves because they are capable of growing in freshwater. The former prevents excess respiratory losses while the latter prevents invasion and competition from non-halophytes.(2) Mangroves are obligate halophytes, i.e. salt is necessary for their growth. Mangroves cannot survive in freshwater permanently and salt water is a physiological requirement. Up to now, mangroves are usually considered as facultative halophytes. In this review, we provided five lines of evidence to evaluate these two contradictory views: (1) the results of laboratory culture experiments and field investigations; (2) the viviparous nature of mangroves; (3) the salt accumulation of mangroves under freshwater or low salinity; (4) the effect of salinity on the photosynthetic rate and in vitro enzyme activities, and (5) the effects of salinity fluctuation on mangrove growth and physiology. Contrary to widely accepted view, our evaluations of the aforementioned evidence suggest that mangroves are obligate halophytes. Mangroves can grow in freshwater for a limited time by drawing upon the nutrients and salt reserves in their hypocotyls while prolonged culture in freshwater is fatal to them. Mangroves have the ability to absorb Na ? and Clrapidly and preferentially under low-salinity conditions. Not all of the enzymes in mangroves are sensitive to salt. In fact, the activities of some enzymes are even stimulated by low or moderate salinity. Plants grown under constant salinity in a laboratory setting are unlikely to behave in the same way as those in their natural habitat with fluctuating salinity. Thus, studies on the effects of freshwater or low salinity and salinity fluctuation on mangroves, as well as the physiological mechanisms that allow maintenance of function under fluctuating salinity conditions should be strengthened in future research.

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Stimulatory effects of sea salts on cell growth in liquid culture of Avicenniaceae mangrove

Cited by 12 publications

References 12 publications

Cellular Calcium Distribution Modulates the Growth of Callus and Protoplasts of Halophyte Mangrove Plant, Avicennia Alba - an X-ray Microanalysis

Cellular Calcium Distribution Modulates the Growth of Callus and Protoplasts of Halophyte Mangrove Plant, Avicennia Alba - an X-ray Microanalysis

Evaluation of an Anthocyanin, Cyanidin 3,5-di-O-glucoside, as an Allelochemical in Red Callus of a Mangrove Sonneratia ovata, Using Protoplast Co-Culture Bioassay Method with Digital Image Analysis

Mangroves: obligate or facultative halophytes? A review

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