Stimulatory effects of the fast setting and suitable degrading Ca–Si–Mg cement on both cementogenesis and angiogenesis differentiation of human periodontal ligament cells

Abstract

The purpose of this study is to develop a fast setting and suitable degrading Mg-calcium silicate cement (Mg-CS) and a mechanism using Mg ions to stimulate human periodontal ligament cells (hPDLCs).Mechanical strength and stability have been determined by testing the diametral tensile strength; the degradation of cements has been measured by ascertaining the number of ions released in simulated body fluid. Other cell characteristics such as proliferation, differentiation and mineralization, and hPDLCs when cultured on cement surfaces were also examined. The results show that the degradation rate of Mg-CS cements depends on the Mg content in CS. Regarding in vitro bioactivity, the CS cements were covered with clusters of apatite spherulites after immersion for 30 days, while there was less formation of apatite spherulites on the Mg-rich cement surfaces. In addition, researchers also explored the effects of Mg ions on the cementogenesis and angiogenesis differentiation of hPDLCs in comparison with pure CS cement. The proliferation, alkaline phosphatase, cementogenesis-related proteins (CEMP1 and CAP), and angiogenesis-related protein (vWF and ang-1) secretion of hPDLCs were significantly stimulated when the Mg ion concentration of the medium was increased. The research results also suggest that Mg-CS cements with this modified composition stimulate hPDLC behaviour and so may be good biomaterials for bone substitutes and hard tissue regeneration applications as they stimulate cementogenesis/angiogenesis.

The effects of Biodentine/polycaprolactone three‐dimensional‐scaffold with odontogenesis properties on human dental pulp cells

“…Although the mechanism of the composite scaffolds‐induced differentiation and mineralization of hDPCs is not clear yet, it seems reasonable to postulate that the release of ionic products from Biodentine, together with the apatite‐forming ability, might play vital roles in manipulating the differentiation and mineralization of hDPCs (Chen et al . , ). Considering the excellent bioactivity and biological performance of the composite, the bioactive scaffold fabricated with 3D printing techniques could be a suitable scaffold for guiding dental and bone regeneration.…”

Effect of Bone Morphogenic Protein-2-Loaded Mesoporous Strontium Substitution Calcium Silicate/Recycled Fish Gelatin 3D Cell-Laden Scaffold for Bone Tissue Engineering

“…Among the numerous types of available biomaterials, ceramics are among the more prominent candidates for bone substitutes. Of which, calcium silicate (CS) is one of the more popular bioceramics due to its excellent biodegradation and osteoconduction ability as compared to the rest of the ceramics [8][9][10]. Recently, bone cements made up of calcium silicate received great attention from researchers as reports indicated that CS had the capability to release therapeutic ions such as Si and Ca ions [11].…”

<p>Macro-Microporous Surface with Sulfonic Acid Groups and Micro-Nano Structures of PEEK/Nano Magnesium Silicate Composite Exhibiting Antibacterial Activity and Inducing Cell Responses</p>

“…The improvements of apatite formation were attributed to more n-MS exposing on the surfaces of both PCP and PCPS, which induced more apatite formation. The mechanism of apatite formation on these samples was similar to that of the n-MS based composites in SBF, 28 which was shown as following: After immersed into SBF, the Ca ions released from the samples due to the slight dissolution of n-MS, which exchanged with H 3 O + in solution, and then Si-OH formed. After that, the Ca 2+ was attracted onto the sample surface by electrostatic action, and then PO 4…”