Stimulatory responses in α‐ and β‐cells by near‐infrared (810 nm) photobiomodulation

Abstract

Significant efforts have been committed to better understand and regulate insulin secretion as it has direct implications on diabetes. The first phase of biphasic insulin secretion in response to glucose lasts about 10 minutes, followed by a more sustained release persisting several hours. Attenuated insulin release in the first phase is typically associated with abnormal β‐cells. While near‐infrared photobiomodulation (PBM) demonstrates potential for multiple therapeutic applications, photostimulatory effects on α‐ and β‐cells remain to be further elucidated. Herein, we demonstrate that 810 nm PBM exposure at fluence of 9 J/cm2 can elevate the intracellular reactive oxygen species within 15 minutes following photostimulation. In addition, calcium spiking showed an approximately 3‐fold increase in both ATC1 (α‐cells) and BTC6 (β‐cells) and correlates with hormone secretion in response to PBM stimulation. Our findings could lay a foundation for the development of non‐biologic therapeutics that can augment islet transplantation.

Low-power infrared laser on survival, proliferation, and reactive oxygen species levels in Escherichia coli cultures

“…Crous et al [43] reported an increase in ROS production in adipose-derived mesenchymal stem cells exposed to a low-power infrared (825 nm) laser in continuous-wave emission mode. Liebman et al [44] demonstrated that exposure to a low-power infrared (810 nm) laser elevated the intracellular ROS levels. In addition, Gholami et al [45] reported an increase in ROS levels in periodontal ligament stem cells after exposure to a low-power pulsed infrared (940 nm) laser.…”

Diabetes in spotlight: current knowledge and perspectives of photobiomodulation utilization

“…Liebman et al. ( 165 ) reported improvement of insulin secretion of beta cells and glucagon of alpha cells associated with a rise of calcium activity. Irani et al.…”

BetaBuddy: An automated end-to-end computer vision pipeline for analysis of calcium fluorescence dynamics in β-cells

“…Pharmacological stimulation has been shown to increase calcium activity through drugs such as tolbutamide, which closes the K ATP channels of the β-cells [48,49]. In addition to EFS, noninvasive stimulation can occur through applications such as photobiomodulation (PBM) therapy [7]. Using this pipeline, the direct comparison of changes in calcium dynamics between different stimuli can now readily be explored.…”

“…The main function of β-cells is the production and secretion of insulin. The release of this hormone is triggered by an increase in ATP [7] as the blood becomes oversaturated with glucose. As intense research efforts continue to explore this disease, many of the mechanisms behind insulin secretion are yet to be fully understood.…”