Stimuli-Responsive DNA-Based Hydrogels: From Basic Principles to Applications

Kahn, Jason S.; Hu, Yuwei; Willner, Itamar

doi:10.1021/acs.accounts.6b00542

Cited by 387 publications

(301 citation statements)

References 55 publications

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“…The leaves of Phaseolus vulgaris L., Pisum sativum L., Vicia faba L., and Brassica napus L. became less hydrophilic following exposure to acid rain 3 . Stimuli-responsive materials, which are functionally similar in wettability to natural surfaces, have been developed for bioseparation 4 , drug delivery systems 5,6 , cell-based diagnostics 7 , biosensing 8,9 , and so on 10,11 . By combining stimuli-responsive materials with microstructures or nanostructures, smart switching between superhydrophobic and superhydrophilic properties has been realized in the last two decades [12][13][14] .…”

Section: Introductionmentioning

confidence: 99%

Naked-eye point-of-care testing platform based on a pH-responsive superwetting surface: toward the non-invasive detection of glucose

et al. 2018

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Herein, we demonstrate a contact angle (CA)-based naked-eye point-of-care testing platform with rapid pH-responsive superwettability that can switch between superhydrophilic and superhydrophobic properties for quantitative biosensing. The CA of the droplet on the pH-responsive surface approached~0°at pH 1 and conversely reached 161.4°± 6.2°at pH 13. We realized the sensitive detection of the pH, urea, and glucose by monitoring the changes in the CA. The traditional invasive diagnosis of diabetes causes pain and brings the risk of infections, such as acquired immune deficiency syndrome (AIDS), hepatitis B, hepatitis C, and syphilis, to the user. To address this issue, we implemented a method for the non-invasive diagnosis of diabetes in human saliva and urine, which avoided the significant drawbacks mentioned above. The accuracy of this method was demonstrated by comparing the results with those from commercial glucometers and theoretical calculations. Interestingly, we successfully monitored glucose levels in sweat before, during, and after cycling. The sensing performance was barely influenced by the temperature, elevation, and droplet color, illustrating promise for expansion to hundreds of millions of potential customers, especially those with color blindness or color weakness. Given its low cost, lack of instruments, and rapid response (within 1 s), this strategy might overcome the limitations of the mechanical stability and durability of superwettable materials and thus might extend the industrial-scale application of bioinspired superwettable systems.

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Section: Introductionmentioning

confidence: 99%

Naked-eye point-of-care testing platform based on a pH-responsive superwetting surface: toward the non-invasive detection of glucose

et al. 2018

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“…[3][4][5] In addition to simple duplexes or single-stranded DNA, G-quadruplexes, unique tetrameric higher-order structures of DNA, started to be found in interesting hydrogel systems. In this mini-review, recent hydrogels utilizing G-quadruplexes are briefly explained.…”

Section: Introductionmentioning

confidence: 99%

“…Among them, Willner's group extensively studied stimuli-responsive hydrogels utilizing G-quadruplexes. 5,[16][17][18][19] They first prepared acrydite-DNA conjugate with relatively short DNA portion, 5-mer dAAGGG to polymerize poly-AAm bearing DNA strands. 16 Addition of K + and hemin to the solution of the polymer triggered formation of hydrogels crosslinked by four-stranded hemin-G-quadruplex DNAzymes.…”

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confidence: 99%

Hydrogels Utilizing G-Quadruplexes

Kuzuya¹

2017

MOJPS

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“…The scientific challenges and future perspectives of nano-capsules and micro-capsules in nanomedicine are highlighted. The reconfiguration principles were used to selectively cleave or isomerize DNA-origami oligomers, [57] to stimulate reversible hydrogel-to-solution transitions, [58][59][60] to switch catalytic cascades of enzymes, [61] and to switch the catalytic functions of DNAzymes. For example, disulfide bridges were reported to stabilize DNA duplex structures, while the reduction of the disulfide by thiols led to the separation of the duplex structures.…”

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confidence: 99%

Synthesis and Applications of Stimuli‐Responsive DNA‐Based Nano‐ and Micro‐Sized Capsules

Liao

Willner

2017

Adv Funct Materials

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Stimuli-responsive, drug-loaded, DNA-based nano-and micro-capsules attract scientific interest as signal-triggered carriers for controlled drug release. The methods to construct the nano-/micro-capsules involve i) the layer-by-layer deposition of signal-reconfigurable DNA shells on drugloaded microparticles acting as templates, followed by dissolution of the core templates; ii) the assembly of three-dimensional capsules composed of reconfigurable DNA origami units; and iii) the synthesis of stimuli-responsive drug-loaded capsules stabilized by DNA−polymer hydrogels. Triggers to unlock the nano-/micro-capsules include enzymes, pH, light, aptamer−ligand complexes, and redox agents. The capsules are loaded with fluorescent polymers, metal nanoparticles, proteins or semiconductor quantum dots as drug models, with anti-cancer drugs, e.g., doxorubicin, or with antibodies inhibiting cellular networks or enzymes over-expressed in cancer cells. The mechanisms for unlocking the nano-/micro-capsules and releasing the drugs are discussed, and the applications of the stimuli-responsive nano-/microcapsules as sense-and-treat systems are addressed. The scientific challenges and future perspectives of nano-capsules and micro-capsules in nanomedicine are highlighted. Drug Delivery groups or duplex DNA or G-quadruplex by photoactive groups may lead to the triggered separation of duplex DNA or G-quadruplex structures ( Figure 1D). For example, disulfide bridges were reported to stabilize DNA duplex structures, while the reduction of the disulfide by thiols led to the separation of the duplex structures. [24][25][26] Also, trans-azobenzene photoisomerizable units linked covalently to duplex nucleic acids or associated with G-quadruplexes via supramolecular interactions, were reported to stabilize these structures, while the cis-azobencene units lack binding affinities (or stabilization effects) to these structures. [27][28][29][30] Photoisomerization of the trans-azobenzene units to the cis-azobenzene configuration, which lacks affinity toward the duplex DNA, results in the release of the photoactive units, in the weakening of the duplex structures, and eventually in their separation. By the cyclic photoisomerization of the photoactive azobenzene units, the double-stranded nucleic acid is then switched between stable and less stable configurations. Alternatively, photodegradable o-nitrobenzyl phosphate ester groups can be incorporated into the duplex DNA structure, and the light-induced separation of the phosphate units may lead to the separation of the duplex DNA. [31] Finally, sequence-specific nucleic acids, known as aptamers, reveal selective recognition properties toward low-molecular-weight substrates (e.g., ATP, cocaine) [32,33] or macromolecular ligands (e.g., thrombin, VEGF). [34,35] The formation of high-affinity complexes between the aptamer and the ligand may then provide a mechanism to separate duplex nucleic acid hybrids [36] (Figure 1E).The structural features of nucleic acids and the possibilities to reconfigure DNA ...

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Stimuli-Responsive DNA-Based Hydrogels: From Basic Principles to Applications

Cited by 387 publications

References 55 publications

Naked-eye point-of-care testing platform based on a pH-responsive superwetting surface: toward the non-invasive detection of glucose

Naked-eye point-of-care testing platform based on a pH-responsive superwetting surface: toward the non-invasive detection of glucose

Hydrogels Utilizing G-Quadruplexes

Synthesis and Applications of Stimuli‐Responsive DNA‐Based Nano‐ and Micro‐Sized Capsules

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