STK3 Suppresses Ovarian Cancer Progression by Activating NF-κB Signaling to Recruit CD8+ T-Cells

Abstract

Serine/threonine protein kinase-3 (STK3) is a critical molecule of the Hippo pathway but little is known about its biological functions in the ovarian cancer development. We demonstrated the roles of STK3 in ovarian cancer. Existing databases were used to study the expression profile of STK3. STK3 was significantly downregulated in OC patients, and the low STK3 expression was correlated with a poor prognosis. In vitro cell proliferation, apoptosis, and migration assays, and in vivo subcutaneous xenograft tumor models were used to determine the roles of STK3. The overexpression of STK3 significantly inhibited cell proliferation, apoptosis, and migration of ovarian cancer cells in vitro and tumor growth in vivo. Bisulfite sequencing PCR analysis was performed to validate the methylation of STK3 in ovarian cancer. RNA sequencing and gene set enrichment analysis (GSEA) were used to compare the transcriptome changes in the STK3 overexpression ovarian cancer and control cells. The signaling pathway was analyzed by western blotting. STK3 promoted the migration of CD8+ T-cells by activating nuclear transcription factor κB (NF-κB) signaling. STK3 is a potential predictor of OC. It plays an important role in suppressing tumor growth of ovarian cancer and in chemotaxis of CD8+ T-cells.

The Integrative Studies on the Functional A-to-I RNA Editing Events in Human Cancers

“…The lost regulation seems to cause the increased expression of APOL1 and indirectly lead to the reduced expression of ZNF280D with long non-coding RNA (lncRNA) transcripts in uterine corpus endometrial carcinoma (UCEC), due to their competing relationships ( Figure 6 D). For these two genes, several recent studies reported the induction function of APOL1 in autophagy [15] to probably promote tumor cell growth and proliferation [31] , and the fusion possibility of ZNF280D with the TSG of STK3 [32] , [33] to involve in cancer. Besides, the DEG analysis also revealed the up-regulation of APOL1 and down-regulation of ZNF280D in the UCEC tumor samples ( Figure 6 E).…”

The Integrative Studies on the Functional A-to-I RNA Editing Events in Human Cancers

“…6D). For these two genes, several recent studies reported the induction function of APOL1 in autophagy (16) to probably promote tumor cell growth and proliferation (36), and the fusion possibility of ZNF280D with tumor suppressor gene of STK3 (37, 38) to involve in cancer. Besides, the DEG analysis also revealed the up-regulation of APOL1 and down-regulation of ZNF280D in the UCEC tumor samples (Fig.…”

Population genetic characteristics of Hainan medaka with whole-genome resequencing

“…For example, we found that many genes are closely related to cell growth and death. The expression of stk3 , ing3 , trit1, and laptm4b may inhibit cell growth and induce apoptosis ( Golovko et al, 2000 ; Nagashima et al, 2003 ; Yarham et al, 2014 ; Blom et al, 2015 ; Wang et al, 2020 ), and cep192 and bub1b may play an important role in mitosis ( Chan et al, 1999 ; Zhu et al, 2008 ; Joukov et al, 2014 ). Further research is required to better understand their roles and possible mechanisms.…”