STMS (sequence tagged microsatellite site) molecular markers as a valuable tool to confirm controlled crosses in chickpea (Cicer arietinum L.) breeding programs

Caballo, Cristina; Castro, P.; Gil, J.; Izquierdo, I.; Millán, Teresa; Rubio, J.

doi:10.1007/s10681-018-2314-0

Cited by 9 publications

(4 citation statements)

References 32 publications

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“…Ochatt et al [82] confirmed the hybrids of interspecific crosses obtained from P. sativum × P. fulvum by molecular markers. Caballo et al [21] reliably tested the confirmation of F 1 hybrids with sequence-tagged microsatellite site (STMS) markers in intraspecific crosses of chickpea (C. arietinum L.). Morais et al [83] utilized SSR to evaluate the efficacy of controlled crossings in common bean.…”

Section: Discussionmentioning

confidence: 99%

“…Differentiation of true hybrid and non-hybrid F 1 plants based on morphological traits may be impossible due to the similar morphological traits, especially in interspecific crosses, e.g., most of the morphological traits of the wild tall pea (P. elatius) are similar to field pea, P. arvense [1]. If there are no adequate dominant morphological traits among parents, the use of molecular markers is a fast and reliable option in food legumes [19][20][21] for the elimination of non-hybrid F 1 plants [21]. Using molecular markers to distinguish the true hybrid plants among the F 1 progeny requires knowledge, specific lab equipment for molecular techniques, time, and low costs per sample.…”

Section: Introductionmentioning

confidence: 99%

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The Fastest and Most Reliable Identification of True Hybrids in the Genus Pisum L.

Sari,

Eker,

Sari

et al. 2023

Life

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After crosses, the identification of true hybrids is not only the most important step in the initiation of a breeding program but also plays a crucial role in the improvement of hybrid varieties. However, current morphological or molecular-based hybrid identification methods are time-consuming and costly approaches that require knowledge and skill, as well as specific lab equipment. In the current study, xenia, direct or immediate effect of pollen on seeds was used to identify true hybrids in the genus Pisum L. for the first time without growing F1 plants. The current study was therefore aimed to (i) elucidate the xenia effect on seeds in intra- and interspecific crosses between P. sativum L. subsp. sativum var. sativum or var. arvense L. Poir. and its wild relatives, including P. sativum subsp. elatius (M. Bieb.) Aschers & Graebn. and P. fulvum Sibth. & Sm., and (ii) illuminate the beneficialness of the xenia effect in a practical improvement of the genus Pisum L. The pea cultivars, including P. sativum subsp. sativum var. sativum and P. sativum subsp. sativum var. arvense, were therefore crossed with P. sativum subsp. elatius and P. fulvum, and the occurrence of the xenia effect was studied on the seeds of fertilized female plants immediately after the crosses. It was concluded that using the xenia effect for the early detection of true hybrid immediately after crossing was not only the fastest, most reliable, and least expensive option as early selection criteria, but that xenia also provided information about dominant seed and pod traits after double fertilization.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Fastest and Most Reliable Identification of True Hybrids in the Genus Pisum L.

Sari,

Eker,

Sari

et al. 2023

Life

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“…Morphological characters are not always suitable to validate the hybrid nature of plants obtained from crosses carried out in the plant breeding programs. Nowadays, molecular markers are commonly used to assess the hybrid nature of cross derived plants (Pereira et al, 2016;Caballo et al, 2018). In asparagus, Valente et al (2009) reported the use of RAPD markers in the assessment of genomic introgression of A. acutifolius and A. maritimus into .…”

Section: Introductionmentioning

confidence: 99%

New hybrids between cultivated and wild species of asparagus (Asparagus spp.) and their validation by SSR markers

Ranjbar¹,

Ghahremani²,

Mousavizadeh³

et al. 2022

Europ.J.Hortic.Sci.

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Garden asparagus ( L., 2n =2x = 20) is an important vegetable crop cultivated worldwide. A narrow genetic base has been pointed out in the current cultivars. Wild related asparagus species could be a valuable resource to extend genetic variation of this crop, but their use have been scarce between cultivated and wild asparagus species, A. persicus (2x) and A. breslerianus (8x), were obtained by hand-pollination. The genetic origin of hybrid plants loid hybrids between cultivated asparagus and both A. persicus and a wild accession of were detected by SSR markers in progenies obtained from open pollination. In this case, the genetic polymorphism among 21 asparagus accessions belong to 10 dioecious species ( (2x, 4x, 8x, 10x), A. verticillatus (2x), A. breslerianus (8x), A. brachyphyllus (6x), A. pseudoscaber (6x), A. acutifolius (4x), A. persicus (2x), A. macrorrhizus (12x), A. maritimus (6x) and A. prostratus (4x open pollination condition were analyzed by using 26 amplicon were found among the accessions. The utility rate of this information for detecting of hybrid plants was tested by employing progenies from the diploid species included A. persicus, wild accession of A. ofand A. verticillatus. Fifteen and nine hybrid plants were detected in progenies from A. persicus and wild accession of respectively, whereas no

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“…It provides inaccurate progenies that can adversely affect all stages of future breeding programs. These problems can be overcome with molecular marker assistance (Caballo et al, 2018;Chauhan et al, 2021). By analyzing the allelic profile of the parents, molecular markers quickly and accurately identify self-fertilized progenies, distinguishing them from hybrid progenies (Conceição et al, 2011;Stetter et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Combining Ability and Molecular Marker Approach Identified Genetic Resources to Improve Agronomic Performance in Coffea arabica Breeding

Medeiros

Caixeta

Oliveira

et al. 2021

Front. Sustain. Food Syst.

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Plant breeding aims to develop cultivars with good agronomic traits through gene recombination and elite genotype selection. To support Coffea arabica breeding programs and assist parent selection, molecular characterization, genetic diversity (GD) analyses, and circulating diallel studies were strategically integrated to develop new cultivars. Molecular markers were used to assess the GD of 76 candidate parents and verify the crossing of potential F1 hybrids. Based on the complementary agronomic traits and genetic distance, eight elite parents were selected for circulating diallel analysis. The parents and 12 hybrids were evaluated based on 10 morpho-agronomic traits. For each trait, the effects of general and specific combining abilities, as well as the averages of the parents, hybrids, and predicted hybrids, were estimated. Crosses that maximize the genetic gains for the main agronomic traits of C. arabica were identified. Joint analysis of phenotypic and molecular data was used to estimate the correlation between molecular GD, phenotypic diversity (PD), phenotypic mean, and combining ability. The selection of parents that optimize the allele combination for the important traits of C. arabica is discussed in detail.

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STMS (sequence tagged microsatellite site) molecular markers as a valuable tool to confirm controlled crosses in chickpea (Cicer arietinum L.) breeding programs

Cited by 9 publications

References 32 publications

The Fastest and Most Reliable Identification of True Hybrids in the Genus Pisum L.

The Fastest and Most Reliable Identification of True Hybrids in the Genus Pisum L.

New hybrids between cultivated and wild species of asparagus (Asparagus spp.) and their validation by SSR markers

Combining Ability and Molecular Marker Approach Identified Genetic Resources to Improve Agronomic Performance in Coffea arabica Breeding

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