Stochastic sensing of proteins with receptor-modified solid-state nanopores

Wei, Ruoshan; Gatterdam, Volker; Wieneke, Ralph; Tampé, Robert; Rant, Ulrich

doi:10.1038/nnano.2012.24

Cited by 451 publications

(439 citation statements)

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“…Molecular delivery is demonstrated down to picomolar concentrations (<10 6 DNA molecules in sub-microliter volumes), advancing high-sensitivity detection and delivery of unamplified samples using nanopipettes. Additionally, molecules can be controllably transported back and forth prior to reaching the tip of the nanopipette by using asymmetric voltage pulses, acting as an "on-off switch" for on-demand molecular delivery.…”

Section: Introductionmentioning

confidence: 99%

On-Demand Delivery of Single DNA Molecules Using Nanopipets

et al. 2015

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Understanding the behavioral properties of single molecules or larger scale populations interacting with single molecules is currently a hotly pursued topic in nanotechnology. This arises from the potential such techniques have in relation to applications such as targeted drug delivery, early stage detection of disease, and drug screening. Although label and label-free single molecule detection strategies have existed for a number of years, currently lacking are efficient methods for the controllable delivery of single molecules in aqueous environments. In this article we show both experimentally and from simulations that nanopipets in conjunction with asymmetric voltage pulses can be used for label-free detection and delivery of single molecules through the tip of a nanopipet with "on-demand" timing resolution. This was demonstrated by controllable delivery of 5 kbp and 10 kbp DNA molecules from solutions with concentrations as low as 3 pM.

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Section: Introductionmentioning

confidence: 99%

On-Demand Delivery of Single DNA Molecules Using Nanopipets

et al. 2015

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“…7,14 Some studies investigated the influence of lipids and the antibody coating of nanopores which makes the nanopore specific for the passing molecule and delays their translocation time. [15][16][17][18] Biologically motivated protein detection mechanisms were tested by attaching DNA and RNA aptamers to nanopores in order to selectively detect proteins. [19][20][21][22] The quantity and spectrum of research demonstrates that protein detection with nanopores is a viable technique currently attracting academic interest.…”

Section: Introductionmentioning

confidence: 99%

Probing the size of proteins with glass nanopores

et al. 2014

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a Single molecule studies using nanopores have gained attention due to the ability to sense single molecules in aqueous solution without the need to label them. In this study, short DNA molecules and proteins were detected with glass nanopores, whose sensitivity was enhanced by electron reshaping which decreased the nanopore diameter and created geometries with a reduced sensing length. Further, proteins having molecular weights (MW) ranging from 12 kDa to 480 kDa were detected, which showed that their corresponding current peak amplitude changes according to their MW. In the case of the 12 kDa ComEA protein, its DNA-binding properties to an 800 bp long DNA molecule was investigated. Moreover, the influence of the pH on the charge of the protein was demonstrated by showing a change in the translocation direction. This work emphasizes the wide spectrum of detectable molecules using nanopores from glass nanocapillaries, which stand out because of their inexpensive, lithography-free, and rapid manufacturing process.

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“…Fabrication of the membranes from solid-state materials presents obvious advantages over their soft matter counterparts, such as very high stability, adjustable surface properties, and the potential for integration into devices and networks. Indeed, development of mechanically and chemically robust solid-state nanopores 2 and nanochannels 3 has already been a rapidly growing area of research due to various practical applications, such as single molecule sensors, 4 energy conversion, 5 and desalination. 6 One of the most important characteristics in biological ion channels is its selectivity, which allows only specific kind of ions to pass through.…”

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confidence: 99%

Electric Field Modulation of the Membrane Potential in Solid-State Ion Channels

Guan

Reed

2012

Nano Lett.

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Biological ion channels are molecular devices that allow a rapid flow of ions across the cell membrane. Normal physiological functions, such as generating action potentials for cell-to-cell communication, are highly dependent on ion channels that can open and close in response to external stimuli for regulating ion permeation. Mimicking these biological functions using synthetic structures is a rapidly progressing yet challenging area. Here we report the electric field modulation of the membrane potential phenomena in mechanically and chemically robust solid-state ion channels, an abiotic analogue to the voltage-gated ion channels in living systems. To understand the complex physicochemical processes in the electric field regulated membrane potential behavior, both quasi-static and transient characteristics of converting transmembrane ion gradients into electric potential are investigated. It is found that the transmembrane potential can be adequately tuned by an external electrical stimulation, thanks to the unique properties of the voltage-regulated selective ion transport through a nanoscale channel.

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Stochastic sensing of proteins with receptor-modified solid-state nanopores

Cited by 451 publications

References 50 publications

On-Demand Delivery of Single DNA Molecules Using Nanopipets

On-Demand Delivery of Single DNA Molecules Using Nanopipets

Probing the size of proteins with glass nanopores

Electric Field Modulation of the Membrane Potential in Solid-State Ion Channels

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