Stock indexing and Potato virus Y elimination from potato plants cultivated in vitro

Nascimento, Luciana Cordeiro do; Pio-Ribeiro, Gilvan; Willadino, Lília; Andrade, Gilcifran Prestes de

doi:10.1590/s0103-90162003000300017

Cited by 37 publications

(33 citation statements)

References 27 publications

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“…2). (1,10,14), Prunus necrotic ringspot virus-PNRSV from herbaceous Begonia spp. plants (17) and woody Prunus mahaleb plants The effi ciency of ribavirin in the elimination of plant viruses is subject to ribavirin concentrations, type of host plant genetic material, and type of virus.…”

Section: Serological and Ic-rt-pcr Testing Of The Treated Plum Materialsmentioning

confidence: 99%

“…plants (17) and woody Prunus mahaleb plants The effi ciency of ribavirin in the elimination of plant viruses is subject to ribavirin concentrations, type of host plant genetic material, and type of virus. Nascimento et al (14) and Verma et al (17) reported that by chemotehrapy in vitro, with application of 20 mg l -1 of ribavirin, in the combination of potato and PVY, and Begonia spp. and PNRSV resulted in 55.5% (re-testing by DAS-ELISA) and 45% (re-testing by RT-PCR) of virus-free plants respectively.…”

Section: Serological and Ic-rt-pcr Testing Of The Treated Plum Materialsmentioning

confidence: 99%

“…Although not widely used, chemotherapy has been successfully employed in obtaining virus-free cultures of economically important crops, such as potato, apple, strawberry, Prunus spp., and sugarcane (1,2,6,7,8,9,10,11,14,15,16).…”

Section: Introductionmentioning

confidence: 99%

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In VitroProduction ofPlum Pox Virus- Free Plums by Chemotherapy with Ribavirin

Paunović

Ružić

Vujović

et al. 2007

Biotechnology & Biotechnological Equipment

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Section: Serological and Ic-rt-pcr Testing Of The Treated Plum Materialsmentioning

confidence: 99%

Section: Serological and Ic-rt-pcr Testing Of The Treated Plum Materialsmentioning

confidence: 99%

See 1 more Smart Citation

In VitroProduction ofPlum Pox Virus- Free Plums by Chemotherapy with Ribavirin

Paunović

Ružić

Vujović

et al. 2007

Biotechnology & Biotechnological Equipment

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“…Excision of a few specialized cells in the apical meristem increases the frequency of meri-clone regeneration with a high proportion of virus-free plantlets (Nascimento et al, 2003;Rukarwa et al, 2010;Wasswa et al, 2010;Wang and Valkonen, 2008). Plant thermotherapy for enhancing virus elimination in infected stock involves exposing infected explants to 35 to 40°C for 14 to 28 days (Agrios, 2005).…”

Section: Introductionmentioning

confidence: 99%

Effect of thermotherapy duration, virus type and cultivar interactions on elimination of potato viruses X and S in infected seed stocks

Waswa¹,

Kakuhenzire²,

Ochwo-Ssemakula³

2017

Afr. J. Plant Sci.

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Virus infection in potato reduces yield through seed degeneration. This can be reduced by use of virusfree seed tubers. However, novel approaches are required to ensure availability of virus-free stocks, especially in developing countries where the seed potato system are evolving. Consequently, a laboratory experiment was conducted at Kachwekano ZARDI in Uganda to evaluate and determine appropriate procedures for cleaning valuable potato cultivars infected with the most prevalent potato viruses; potato virus X (PVX) and potato virus S (PVS) which are frequent in the potato farming system. Thus, 20 in-vitro potato plantlets from cultivars Victoria, Kinigi and Rwangume each batch infected with either PVX or PVS in three replicates were grown in a thermotherapy chamber for two, three and four weeks at 37 to 40°C in 16 h of light and 30 to 34°C for 8 h of darkness per day. An equal number plantlets and replicates of the same cultivars and virus infection combinations were grown in a standard tissue culture (TC) growth room at 16 to 18°C with 16 h of light without thermotherapy as controls. Results indicated that plantlet survival after in-vitro thermotherapy decreased with increasing duration of heat treatment. Virus elimination efficiency significantly (P ≤ 0.05) differed between heattreated plantlets and the controls but not between the duration of thermotherapy treatment. However, the highest proportion of virus-free plantlets was obtained after three weeks of thermotherapy. Significant (P ≤ 0.05) interactions were observed between thermotherapy duration and virus type on virus elimination efficiency where significantly (P ≤ 0.05) more PVS-free than PVX-free plantlets were obtained at the same thermotherapy duration. Three weeks of thermotherapy of virus-infected in-vitro plantlets, particularly for PVS, offered an equilibrium duration for adequate plantlet survival and maximum meri-clone regeneration to obtain the highest proportion of virus-free plantlets. The use of thermotherapy to obtain a high proportion of PVX-free plantlets from infected potato seed stock showed no clear trend and needs further investigation.

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“…Several investigators (Nascimento et al 2003;Guta et al 2010;Panattoni et al 2013aPanattoni et al , 2014Skiada et al 2013;Guazzelli et al 2015) have found that some animal IMPDH inhibitors have antiviral activity in plants and, based on the data, the mechanism of antiviral action found in animals can occur in plants as well. This fact highlights the important role of IMPDH in viral replication.…”

Section: Introductionmentioning

confidence: 99%

Modulation of viral infection in plants by exogenous guanosine

et al. 2015

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To evaluate the role of purines in antiviral treatments in plants, ribavirin (RB) and tiazofurin (TZ) were applied in combination with guanosine (GS) or adenosine (AS) in in vitro grapevine or tobacco explants infected by Grapevine leafroll associated virus 3 (GLRaV-3) and Cucumber mosaic virus (CMV), respectively. Using a microelectrochemical (trans-plasma membrane electron transport, t-PMET) technique, in vivo assay of free reduced nicotinamide adenine dinucleotide (NADH) was also carried out to estimate the inosine monophosphate dehydrogenase inhibition caused by drugs. Antiviral effectiveness of TZ, evaluated as virus-free explants or virus copies, was significantly hindered by GS in both species, while AS did not interfere with the drugs. GS, but not AS, slightly hindered the antiviral effectiveness of RB. With regard to NADH tests, t-PMET inhibition caused by RB and TZ was dose dependent and the interference of drugs with the NAD ? /NADH conversion was confirmed by NADH content. Findings indicate that exogenous GS up to 0.50 mM replenished the GS pool depleted by drugs, contrasting antiviral action. At higher doses of GS, the TZ antiviral action was completely inhibited and exogenous GS caused a feedback that reduced t-PMET activity. The reversal was partially against RB, suggesting that the reduction of the GS pool contributed to the antiviral activity of RB, but it was not the only cause of antiviral effectiveness.

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Stock indexing and Potato virus Y elimination from potato plants cultivated in vitro

Cited by 37 publications

References 27 publications

In VitroProduction ofPlum Pox Virus- Free Plums by Chemotherapy with Ribavirin

In VitroProduction ofPlum Pox Virus- Free Plums by Chemotherapy with Ribavirin

Effect of thermotherapy duration, virus type and cultivar interactions on elimination of potato viruses X and S in infected seed stocks

Modulation of viral infection in plants by exogenous guanosine

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