2001
DOI: 10.1111/j.1574-6976.2001.tb00570.x
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Stoichiometry and compartmentation of NADH metabolism inSaccharomyces cerevisiae

Abstract: In Saccharomyces cerevisiae, reduction of NAD(+) to NADH occurs in dissimilatory as well as in assimilatory reactions. This review discusses mechanisms for reoxidation of NADH in this yeast, with special emphasis on the metabolic compartmentation that occurs as a consequence of the impermeability of the mitochondrial inner membrane for NADH and NAD(+). At least five mechanisms of NADH reoxidation exist in S. cerevisiae. These are: (1) alcoholic fermentation; (2) glycerol production; (3) respiration of cytosoli… Show more

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Cited by 442 publications
(120 citation statements)
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References 161 publications
(308 reference statements)
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“…Deletion of HAP4 deprives the cells of its excess respiratory capacity, and hence there is ethanol formation at lower glycolytic fluxes. In agreement with our hypothesis, deletion of both NDE1 and NDE2 (external mitochondrial NADH dehydrogenases encoded by NDE1 and NDE2, which oxidize cytosolic NADH and couple it to the respiratory chain) reduced the D crit only by 23% (34,35), which is in sharp contrast to 75% reduction for the hap4⌬ mutant observed here. dos Santos et al (36) reported that overexpression of malic enzyme leads to a decrease in the critical dilution rate by as much as 54% of the WT.…”
supporting
confidence: 92%
“…Deletion of HAP4 deprives the cells of its excess respiratory capacity, and hence there is ethanol formation at lower glycolytic fluxes. In agreement with our hypothesis, deletion of both NDE1 and NDE2 (external mitochondrial NADH dehydrogenases encoded by NDE1 and NDE2, which oxidize cytosolic NADH and couple it to the respiratory chain) reduced the D crit only by 23% (34,35), which is in sharp contrast to 75% reduction for the hap4⌬ mutant observed here. dos Santos et al (36) reported that overexpression of malic enzyme leads to a decrease in the critical dilution rate by as much as 54% of the WT.…”
supporting
confidence: 92%
“…Because the biomass yield for optimal anaerobic growth is 0.1 g of biomass per g of glucose, and the carbon content of biomass is 40% (13), 10%, at most, of the glucose may be incorporated into biomass. Because S. cerevisiae lacks a transhydrogenase, the pools of NADPH and NADH are not linked (14). For each mole of glucose going into biomass, 1 mole of NADPH is required (15).…”
Section: Discussionmentioning
confidence: 99%
“…2 Accordingly, the amount of Tdh1p was increased in the ser3⌬ ser33⌬ double mutant indicating an increased cytosolic NADH/NAD ϩ ratio. However, since the cells were cultured under aerobic conditions, the surplus of NADH formed, both cytosolically and/or in the mitochondria (45,46), should not create a redox problem since it can still be reoxidized in the respiratory chain. This is also reflected by a relatively non-affected growth rate of the double mutant.…”
Section: Phosphoglycerate Dehydrogenases In S Cerevisiaementioning
confidence: 99%