1982
DOI: 10.1007/bf01868482
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Stoichiometry of proton movements coupled to ATP synthesis driven by a pH gradient inStreptococcus lactis

Abstract: An electrochemical potential difference for H+ was established across the plasma membrane of the anaerobe Streptococcus lactis by addition of sulfuric acid to cells suspended in potassium phosphate at pH 8 along with valinomycin or permeant anions. Subsequent acidification of the cell was measured by the distribution of salicyclic acid. A comparison between cells treated or untreated with the inhibitor N,N'-dicyclohexylcarbodiimide was used to reveal that portion of net proton entry attributable to a direct co… Show more

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Cited by 37 publications
(32 citation statements)
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“…Finally, ⌬p was calculated from ⌬G p as follows: Ϫn⌬p ϭ ⌬G p /F, where n is the number of protons translocated by the ATP synthase enzyme per molecule of ATP synthesized and F is the Faraday constant. The value of n is typically reported in the range of two to three for bacteria (23,28,35,45,50), and the results of these studies indicate that n may vary as an inverse (45). This suggests that for the current study, where ATP levels increased upon adhesion to the glass beads, n may lie near the lower end of the reported range.…”
Section: Resultsmentioning
confidence: 99%
“…Finally, ⌬p was calculated from ⌬G p as follows: Ϫn⌬p ϭ ⌬G p /F, where n is the number of protons translocated by the ATP synthase enzyme per molecule of ATP synthesized and F is the Faraday constant. The value of n is typically reported in the range of two to three for bacteria (23,28,35,45,50), and the results of these studies indicate that n may vary as an inverse (45). This suggests that for the current study, where ATP levels increased upon adhesion to the glass beads, n may lie near the lower end of the reported range.…”
Section: Resultsmentioning
confidence: 99%
“…An inverted membrane potential was generated in membrane vesicles of L. mesenteroides fused with liposomes lacking cytochrome c oxidase by the "pH jump" technique (Maloney and Hansen, 1982). The external pH was rapidly dropped from 7.0 to 6.0 by adding sulfuric acid, which created a pH gradient (inside alkaline).…”
Section: Resultsmentioning
confidence: 99%
“…The development of the proton diffusion potential is a consequence of the higher permeability of protons than of the sulfate and phosphate anions (14). In the presence of valinomycin, the diffusion potential was significantly reduced (Fig.…”
mentioning
confidence: 97%