Stomatal Opening Is Induced in Epidermal Peels of Commelina communis L. by GTP Analogs or Pertussis Toxin

Lee, Ho Joung; Tucker, Edward B.; Crain, Richard C.; Lee, Youngsook

doi:10.1104/pp.102.1.95

Cited by 52 publications

(28 citation statements)

References 41 publications

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“…From these experiments it was estimated that iontophoretic injection of ABA resulted in a concentration of 50 to 200 JIM ABA in the guard cell cytosol. The efficiency of ABA microinjection determined here correlates closely with microinjection efficiencies reported for guard cells in recent studies (Gilroy et al, 1990(Gilroy et al, , 1991McAinsh et al, 1990;Lee et al, 1993). These estimates give the cytosolic concentration of microinjected ABA and do not include endogenous ABA in guard cells (Harris et al, 1988).…”

Section: Iontophoretic Microinjection Of Commelina Guard Cellssupporting

confidence: 72%

Evidence for an Extracellular Reception Site for Abscisic Acid in Commelina Guard Cells

1994

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Section: Iontophoretic Microinjection Of Commelina Guard Cellssupporting

confidence: 72%

Evidence for an Extracellular Reception Site for Abscisic Acid in Commelina Guard Cells

1994

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“…Since reduced K ÷ uptake inhibits stomatal opening, these results suggest that GTPTS and the toxins would inhibit stomatal opening in broad bean guard cells under these conditions. In contrast, both GTPTS and pertussis toxin induce stomatal opening in the epidermis of Commelina communis [97]. Again, similar effects of GTPTS and pertussis toxin suggest more than one protein may be involved.…”

Section: G Protein Involvement In Plant Signalling Pathwaysmentioning

confidence: 80%

GTP-binding proteins in plants: new members of an old family

Mā

1994

Plant Mol Biol

199

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Regulatory guanine nucleotide-binding proteins (G proteins) have been studied extensively in animal and microbial organisms, and they are divided into the heterotrimeric and the small (monomeric) classes. Heterotrimeric G proteins are known to mediate signal responses in a variety of pathways in animals and simple eukaryotes, while small G proteins perform diverse functions including signal transduction, secretion, and regulation of cytoskeleton. In recent years, biochemical analyses have produced a large amount of information on the presence and possible functions of G proteins in plants. Further, molecular cloning has clearly demonstrated that plants have both heterotrimeric and small G proteins. Although the functions of the plant heterotrimeric G proteins are yet to be determined, expression analysis of an Arabidopsis G alpha protein suggests that it may be involved in the regulation of cell division and differentiation. In contrast to the very few genes cloned thus far that encode heterotrimeric G proteins in plants, a large number of small G proteins have been identified by molecular cloning from various plants. In addition, several plant small G proteins have been shown to be functional homologues of their counterparts in animals and yeasts. Future studies using a number of approaches are likely to yield insights into the role plant G proteins play.

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“…Absolute values of stomatal opening were low in both treatments, as has been reported previously for submerged peels (H. Lee et al 1993). In two of the mock-ablation experiments and two of the control experiments, stomatal apertures were additionally measured at an intermediate time point, after use of the linear-gradient maker (i.e., after deplasmolysis of the mock-ablated cells) but before the white-light treatment used to induce stomatal opening.…”

Section: Stomatal Openingmentioning

confidence: 94%

A laser microsurgical method of cell wall removal allows detection of largeconductance ion channels in the guard cell plasma membrane

1999

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Application of patch clamp techniques to higher-plant cells has been subject to the limitation that the requisite contact of the patch electrode with the cell membrane necessitates prior enzymatic removal of the plant cell wall. Because the wall is an integral component of plant cells, and because cell-wall-degrading enzymes can disrupt membrane properties, such enzymatic treatments may alter ion channel behavior. We compared ion channel activity in enzymatically isolated protoplasts of Vicia faba guard cells with that found in membranes exposed by a laser microsurgical technique in which only a tiny portion of the cell wall is removed while the rest of the cell remains intact within its tissue environment. "Laser-assisted" patch clamping reveals a new category of high-conductance (130 to 361 pS) ion channels not previously reported in patch clamp studies on plant plasma membranes. These data indicate that ion channels are present in plant membranes that are not detected by conventional patch clamp techniques involving the production of individual plant protoplasts isolated from their tissue environment by enzymatic digestion of the cell wall. Given the large conductances of the channels revealed by laser-assisted patch clamping, we hypothesize that these channels play a significant role in the regulation of ion content and electrical signalling in guard cells.

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Stomatal Opening Is Induced in Epidermal Peels of Commelina communis L. by GTP Analogs or Pertussis Toxin

Cited by 52 publications

References 41 publications

Evidence for an Extracellular Reception Site for Abscisic Acid in Commelina Guard Cells

Evidence for an Extracellular Reception Site for Abscisic Acid in Commelina Guard Cells

GTP-binding proteins in plants: new members of an old family

A laser microsurgical method of cell wall removal allows detection of largeconductance ion channels in the guard cell plasma membrane

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