Storage and stability of compounds labelled with radioisotopes — part II

Bayly, R. J.; Evans, E. A.

doi:10.1002/jlcr.2590030502

Cited by 20 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the same time these large yields could reflect migration of energy or charge, or both, from the cellulose support material to the critical amino acids: previous studies of the modification by X-irradiation of various small molecules adsorbed on to gels indicated that there must be transfer of both electrons and protons from the gels to the adsorbed molecules (Gevantman, 1960;Allen, 1960). Such a possibility might also be consistent with the report by Bayly & Evans (1967) of the extreme lability of sugars adsorbed on to cellulose bases. By contrast, the adsorption of macromolecules on to (or into) porous substrata appears to decrease their radiation-sensitivity (Augenstein, Augenstein & Lippincott, 1960;Augenstein, 1962a;Holladay, Augenstein & Augenstein, 1966;James & Augenstein, 1966).…”

Section: P4supporting

confidence: 91%

A comparison of the effects of ultraviolet and ionizing radiations on trypsin activity and on its constituent amino acids

Burke

Augenstein

1969

Biochemical Journal

View full text Add to dashboard Cite

Photons of 254nm. u.v. light, (60)Co gamma-rays and 1Mev electrons produce different patterns of destruction of individual amino acids in dried films of trypsin and in the corresponding amino acid mixture. For example, in the amino acid mixture u.v. light destroys tyrosine, tryptophan and cystine, whereas in trypsin only cystine is disrupted but with 10 times the initial yield. Further, in the amino acid mixture loss of half-cystine is a simple exponential function of dose, but in trypsin there appear to be two exponential components of the loss with yields that differ by a factor of 35. Both the gamma-rays and electrons destroy half-cystine, tryptophan, histidine and methionine in the amino acid mixture with remarkably high yields, whereas in trypsin doses that destroy almost all of the enzymic activity produce no detectable destruction of amino acid residues. These marked differences between the two preparations show that the radiation-sensitivity of a given amino acid alone and in a protein is different, and suggests that in trypsin there is fairly extensive migration of energy, charge or both with localization of damage at specific sites determined by this enzyme's internal organization. All three types of radiation produce appreciable amounts of ;damaged' (not completely inactivated) molecules which are prevented from reassuming an active configuration by the addition of 5.5m-urea; thiol reagents have a similar effect after bombardment with u.v. light or electrons. The patterns of destruction produced by gamma-rays and by electrons in both the amino acid mixture and in trypsin are different (some of the yields vary by a factor of 30). This result appears to be inconsistent with the popular belief that most of the energy absorbed from gamma-rays is associated with very-high-energy electrons.

show abstract

Section: P4supporting

confidence: 91%

A comparison of the effects of ultraviolet and ionizing radiations on trypsin activity and on its constituent amino acids

Burke

Augenstein

1969

Biochemical Journal

View full text Add to dashboard Cite

show abstract

“…The stabilities of several radiolabeled alkyl halides, hydrocarbons, and cyanides are summarized in Table 1 51,52 . [ 14 C]Methyl iodide decomposes to yield a wine‐red solution (with the color resulting from the production of iodine) 73–76 .…”

Section: Self‐radiolysis Of T2omentioning

confidence: 99%

“…Self‐radiolysis is a significant challenge for radio‐chemists and has necessitated the development of several techniques to stabilize radiolabeled compounds including the addition of stabilizing agents, 32,38,39 dilution with unlabeled compound, and modification of storage conditions (storage in solution or adsorbed on paper) 40–46 . While there have been several review articles written on the subjects of 14 C and 3 H decomposition of organic compounds, 47–54 many of these articles are over 50 years old and, as such, do not include data on the decomposition of recently prepared carbon‐14 and tritium containing compounds. In this paper, a comprehensive of review of the self‐radiolysis of compounds incorporating 3 H and 14 C radiolabels is presented, and the stabilities of the radiolabeled compounds are discussed with respect of functional groups on the radiolabeled compound.…”

Section: Introductionmentioning

confidence: 99%

Review: Self‐radiolysis of compounds containing tritium and carbon‐14

Wolf

2021

Labelled Comp Radiopharmac

View full text Add to dashboard Cite

Energy released from beta emission causes molecules that incorporate tritium (3H) and carbon‐14 (14C) atoms to be less stable than their stable labeled counterparts, thus making radiolabeled compounds prone to self‐radiolysis. This literature review examines the stability of organic compounds incorporating 3H and 14C atoms and discusses stability of the compound with respect to functional groups within the radiolabeled molecules. The goal of this paper is to serve as a reference for the stability of radiolabeled compounds.

show abstract

“…and nucleotides at high molar specific activity (above say 1 Ci/mmole) are given in the Table 2, together with estimates of their decomposition to produce radiochemical impurities (excluding THO) during one month. These estimates are based on recent results from our laboratories which are given in the Table 3, and on previously published data (18).…”

Section: Stability Of Tritiated Nucleic Acid Precursors On Storagementioning

confidence: 99%

Validity of tritium tracers. Stability of tritium atoms in purines, pyrimidines, nucleosides and nucleotides

Evans

Sheppard

Turner

1970

J. Labelled Cpd. Radiopharm.

View full text Add to dashboard Cite

The effect of p H and temperature on the stability of tritium atoms at various positions in purines, pyrimidines, nucleosides and nucleotides is discussed. The importance of recognizing several types of instability is stressed.The .formation of a hydrate on storing solutions of tritiated uridine and other results of the self-radiolysis of tritiated precursors of the nucleic acids are described. Alcohol solutions and low temperatures are recommended for minimising self-decomposition.--

show abstract

Storage and stability of compounds labelled with radioisotopes — part II

Abstract: INTRODUCTION.

Cited by 20 publications

References 18 publications

A comparison of the effects of ultraviolet and ionizing radiations on trypsin activity and on its constituent amino acids

A comparison of the effects of ultraviolet and ionizing radiations on trypsin activity and on its constituent amino acids

Review: Self‐radiolysis of compounds containing tritium and carbon‐14

Validity of tritium tracers. Stability of tritium atoms in purines, pyrimidines, nucleosides and nucleotides

Contact Info

Product

Resources

About