Storage behavior and cryopreservation studies in Indian rough lemon (Citrus jambhiri): a promising rootstock for long-term conservation

Abstract

The study of the seed storage behavior and cryopreservation of embryonic axes was attempted in Citrus jambhiri genotypes using air desiccation-freezing, vitrification, and encapsulation-dehydration methods. Seeds of this species revealed nonorthodox seed storage behavior with moderate seed longevity and sensitivity to desiccation and freezing. Successful cryopreservation was achieved with all three methods using embryonic axes; however, the recovery growth percentage significantly differed among these methods. Cryopreservation was efficient for the embryonic axes desiccated to 13%-15% moisture content. Freezing of embryonic axes at this moisture content in liquid nitrogen showed a good recovery rate of 40%-50%. In the vitrification method, embryonic axes subjected to PVS2 treatment for 40 min had the highest recovery rate of 25% after cryopreservation. In the encapsulation-dehydration method, encapsulated axes precultured on 0.5 M sucrose medium followed by 6 h of desiccation had the highest recovery growth of 30%-60% after cryopreservation. Out of the three methods employed, the encapsulation-dehydration method gave the best recovery values for embryonic axes. High regeneration values of embryos in comparison to embryonic axes prompted us to undertake large-scale cryobanking of C. jambhiri using embryos (seeds without seed coats) and more than 100 accessions of C. jambhiri have been cryostored in the national cryobank. This would further serve the purpose of conserving nucellar embryos with zygotic embryos.

Cryopreservation of Fruit Genetic Resources-A Review

“…Preculturing beads on 0.5 M sucrose followed by 6 h of air desiccation gave maximum survival of axes before and after cryopreservation in IPS-7 and IPS-85, but for accession IPS-120 successful cryopreservation with a highest viability of 66.66 and 50 per cent respectively, was achieved for axes precultured for 40 h in 0.3 M sucrose preculture media, followed by 6 h of desiccation. More than 100 accessions of C. jambhiri have been cryostored in the national cryobank at NBPGR, New Deli (Rohini et al, 2016).…”

Unlocking the Potential of Phloroglucinol and Tocopherol in Improving the Photosynthetic Efficiency, Membrane Stability, and Antioxidant Enzyme Activities of Cryopreserved In Vitro Raised Plantlets of Citrus maxima Merr. And Citrus jambhiri Lush

“…Furthermore, climate change poses an increased risk of intensi cation of plant diseases, putting at risk the world's food supply and natural plant biodiversity (Singh et al 2023). Further, long-term conventional seeds storage for both C. maxima and C. jambhiri is not possible owing the recalcitrant nature of their seeds (Rohini et al 2016;Khopkar et al 2017). Therefore, the aim of this research investigation was to perform an antioxidant or elicitors-assisted cryopreservation of seeds of the two species with the sole purpose to improve the performances of cryo-retreived in vitro raised and clonally propagated plantlets of C. jambhiri and C. maxima.…”

Micropropagation, in-vitro conservation and genetic stability studies in pummelo (Citrus maxima)

“…In Citrus species several explants like shoot tips, stem sections, root sections, leaf sections, stem internodes, epicotyl segments and transverse thin cell layer have been used for micropropagation followed by in vitro conservation and cryopreservation using various techniques in several laboratories of the world (Rohini et al 2016, Liu and Deng 2007, De Carlo and Lambardi 2005.…”