Storage compounds, ABA and fumarase in Fagus sylvatica embryos during stratification

Eliášová, Kateřina; Pešek, Bedřich; Vondráková, Zuzana

doi:10.12657/denbio.074.003

Cited by 4 publications

(4 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phenol compounds often are strongly inhibitory toward seed germination (Han et al, 2001;Samajdar et al, 2018;Zhao et al, 2018). In addition, ABA is an important plant hormone involved in many biological processes, including the regulation of seed dormancy and germination (Bian et al, 2018;Eli a sov a et al, 2015;Samajdar et al, 2018). On the basis of the changes to their relative contents, 1,2,3-benzenetriol and ABA were identified as putative endogenous inhibitors of C. chinensis seed germination.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Dynamic Changes to Endogenous Germination Inhibitors in Cercis chinensis Seeds during Dormancy Release

Gao¹,

Zhu²,

Wang³

et al. 2021

horts

View full text Add to dashboard Cite

The purpose of this study was to determine whether Cercis chinensis seeds contain endogenous germination inhibitors, and if so, to clarify the dynamic changes to the inhibitors during dormancy release. A cabbage seed germination test was conducted to assess the seed extract activities during dormancy release. The endogenous inhibitor components were analyzed by gas chromatography–mass spectrometry and the dynamic changes to the endogenous inhibitors were analyzed by high-performance liquid chromatography. The analyses revealed 1,2,3-benzenetriol (phenolic compound) in the seedcoat is a water-soluble endogenous inhibitor, and the IC50 (the concentration at which 1,2,3-benzenetriol inhibited radicle growth of cabbage seeds by 50%) of 1,2,3-benzenetriol was 51.2 µg⋅mL–1. During dormancy release, the seed 1,2,3-benzenetriol content decreased to 17.7 µg⋅mL–1 (stratified for 60 days) from 561.4 µg⋅mL–1 (control). The abscisic acid (ABA) content exhibited the same tendency, decreasing from 5.6 ng⋅mL–1 to 0.5 ng⋅mL–1 after 60-day stratification. Exogenous ABA was highly inhibitory toward cabbage seed germination, with an IC50 of 1.5 ng⋅mL–1. These results indicate that ABA and 1,2,3-benzenetriol are important endogenous inhibitors in C. chinensis seeds, wherein they regulate seed dormancy, even at low concentrations.

show abstract

Section: Resultsmentioning

confidence: 99%

“…ABA is one of the major plant hormones involved in many biological processes, especially in the regulation of seed dormancy and germination (Bian et al, 2018;Eli a sov a et al, 2015;Samajdar et al, 2018). Earlier studies concluded that ABA sensitivity is correlated with the extent of the dormancy of mature seeds (Bewley and Black, 1994).…”

Section: Discussionmentioning

confidence: 99%

Dynamic Changes to Endogenous Germination Inhibitors in Cercis chinensis Seeds during Dormancy Release

Gao¹,

Zhu²,

Wang³

et al. 2021

horts

View full text Add to dashboard Cite

show abstract

“…Beech seed germination manifested by embryonic axis elongation begins at approximately the 12th week of cold stratification [ 5 ]. Importantly, in beech seeds subjected to germination, protein storage vacuoles have been reported to diminish or disappear after the first week of stratification and were mostly utilized at the final stage of stratification [ 49 ]. In this context, MsrB1 and MsrB2 are suggested to be involved in proper storage material utilization in beech seeds prior to germination.…”

Section: Discussionmentioning

confidence: 99%

Localization and Dynamics of the Methionine Sulfoxide Reductases MsrB1 and MsrB2 in Beech Seeds

Wojciechowska

Bagniewska‐Zadworna

Minicka

et al. 2021

IJMS

View full text Add to dashboard Cite

Beech seeds are produced irregularly, and there is a need for long-term storage of these seeds for forest management practices. Accumulated reactive oxygen species broadly oxidize molecules, including amino acids, such as methionine, thereby contributing to decreased seed viability. Methionine oxidation can be reversed by the activity of methionine sulfoxide reductases (Msrs), which are enzymes involved in the regulation of many developmental processes and stress responses. Two types of Msrs, MsrB1 and MsrB2, were investigated in beech seeds to determine their abundance and localization. MsrB1 and MsrB2 were detected in the cortical cells and the outer area of the vascular cylinder of the embryonic axes as well as in the epidermis and parenchyma cells of cotyledons. The abundances of MsrB1 and MsrB2 decreased during long-term storage. Ultrastructural analyses have demonstrated the accumulation of these proteins in protein storage vacuoles and in the cytoplasm, especially in close proximity to the cell membrane. In silico predictions of possible Msr interactions supported our findings. In this study, we investigate the contribution of MsrB1 and MsrB2 locations in the regulation of seed viability and suggest that MsrB2 is linked with the longevity of beech seeds via association with proper utilization of storage material.

show abstract

“…Beech seeds accumulate lipids, mainly triacylglycerols [53,54] and proteins [54,55]. Cotyledons contained twice as many total proteins and lipids as elongating embryonic axes when germination third phase was initiated.…”

Section: Discussionmentioning

confidence: 99%

Dehydration Sensitivity at the Early Seedling Establishment Stages of the European Beech (Fagus sylvatica L.)

Kalemba

Bagniewska‐Zadworna

Suszka

et al. 2019

Forests

View full text Add to dashboard Cite

Shortage of water is a limiting factor for the growth and development of plants, particularly at early developmental stages. We focused on the European beech (Fagus sylvatica L.), which produces seeds and further seedlings in large intervals of up to ten years. To explore the beech seedling establishment process, six stages referring to embryo expansion were studied to determine sensitivity to dehydration. The characterization of the response of elongating embryonic axes and cotyledons included a viability test before and after dehydration and measurement of the amounts of electrolyte leakage, concentration, and arrangement of storage materials, changes in chaperone proteins related to water deficit, and accumulation of hydrogen peroxide and superoxide anion radicals. Elongating embryonic axes and cotyledons differed in water content, dehydration rates, membrane permeability before and after dehydration, protein, and lipid decomposition pattern, and amount of 44-kDa dehydrin and 22-kDa small heat shock protein (sHSP). Protruding embryonic axes were more sensitive to dehydration than cotyledons, although dehydration caused transient reinduction of three dehydrin-like proteins and sHSP synthesis, which accompany desiccation tolerance. Extended deterioration, including overproduction of hydrogen peroxide and depletion of superoxide anion radicals, was reported in dehydrated embryonic axes longer than 10 mm characterized by highly elevated cellular leakage. The apical part elongating embryonic axes consisting of the radicles was the most sensitive part of the seed to dehydration, and the root apical meristem area was the first to become inviable. The effects of severe dehydration involving ROS imbalance and reduced viability in beech seedlings with embryonic axes longer than 10 mm might help to explain the difficulties in beech seedling establishment observed in drought-affected environments. The conversion of environmental drought into climate-originated oxidative stress affecting beech seedling performance is discussed in this report.

show abstract

Storage compounds, ABA and fumarase in Fagus sylvatica embryos during stratification

Cited by 4 publications

References 26 publications

Dynamic Changes to Endogenous Germination Inhibitors in Cercis chinensis Seeds during Dormancy Release

Dynamic Changes to Endogenous Germination Inhibitors in Cercis chinensis Seeds during Dormancy Release

Localization and Dynamics of the Methionine Sulfoxide Reductases MsrB1 and MsrB2 in Beech Seeds

Dehydration Sensitivity at the Early Seedling Establishment Stages of the European Beech (Fagus sylvatica L.)

Contact Info

Product

Resources

About