Storage conditions of high‐fat diets affect pulmonary inflammation

Kokoszynska, M.; Ubags, Niki; Bivona, Joseph J.; Ventrone, Sebastian; Reed, Leah; Dixon, Anne E.; Wargo, Matthew J.; Poynter, Matthew E.; Suratt, Benjamin T.

doi:10.14814/phy2.15116

Cited by 2 publications

(2 citation statements)

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“…The temperature at which VHFD is stored, for example, differentially alters weight gain and liver damage in mice [ 2 ]. Storage conditions of VHFD also affect pulmonary inflammation in mice [ 3 ]. Thus, the storage and handling practices of VHFD are important variables to consider, control, and report for researchers to avoid experimental confound and prevent problems with replicability and reproducibility.…”

Section: Introductionmentioning

confidence: 99%

Daily replacement of very high-fat diet stabilizes food intake and improves mouse welfare by ensuring food quality

Cordeira

2023

PLoS ONE

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Researchers are obligated to ensure food quality and provide laboratory animals with a palatable diet. Factors influencing the quality and palatability of very high-fat diet (VHFD), a widely used rodent diet, however, are understudied. We conducted experiments to establish best practices for ensuring the quality of VHFD and to improve mouse welfare. We found that VHFD in the food hopper was vulnerable first to dehydration and then oxidation within 7-days, leading to dramatic changes in food intake and food preference behavior in mice. Mitigating dehydration and oxidation of VHFD by replacing food daily, rather than weekly, stabilized feeding behavior without effect on overall cardio-metabolic health. Importantly, daily replacement of VHFD also reduced measures of anxiety-like behavior in the open field test. Refining husbandry practices to include daily replacement of VHFD can therefore ensure VHFD quality and improve animal welfare. Standardizing the practice of daily VHFD replacement may also prevent experimental confound and improve experimental reproducibility and replicability.

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Section: Introductionmentioning

confidence: 99%

Daily replacement of very high-fat diet stabilizes food intake and improves mouse welfare by ensuring food quality

Cordeira

2023

PLoS ONE

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“…Macrophages play an important role in the pathogenesis of IPF. Macrophages form through the phagocytosis of extracellular oxidized phospholipids and produce additional TGF-β, which aggravates pulmonary fibrosis (10). Clinical studies have also noted a strong link between fatty acid metabolism and IPF.…”

Section: Introductionmentioning

confidence: 99%

Identification and immune characteristics of molecular subtypes related to fatty acid metabolism in idiopathic pulmonary fibrosis

Yang

et al. 2022

Front. Nutr.

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BackgroundAlthough fatty acid metabolism has been confirmed to be involved in the pathological process of idiopathic pulmonary fibrosis (IPF), systematic analyses on the immune process mediated by fatty acid metabolism-related genes (FAMRGs) in IPF remain lacking.MethodsThe gene expression data of 315 patients with IPF were obtained from Gene Expression Omnibus database and were divided into the training and verification sets. The core FAMRGs of the training set were identified through weighted gene co-expression network analysis. Then, the fatty acid metabolism-related subtypes in IPF were identified on the basis of k-means unsupervised clustering. The scores of fatty acid metabolism and the expression of the fibrosis biomarkers in different subtypes were compared, and functional enrichment analysis was carried out on the differentially expressed genes between subtypes. A random forest model was used to select important FAMRGs as diagnostic markers for distinguishing between subtypes, and a line chart model was constructed and verified by using other datasets and rat models with different degrees of pulmonary fibrosis. The difference in immune cell infiltration among subtypes was evaluated with CIBERSORT, and the correlation between core diagnostic markers and immune cells were analyzed.ResultsTwenty-four core FAMRGs were differentially expressed between the training set and normal samples, and IPF was divided into two subtypes. Significant differences were observed between the two subtypes in biological processes, such as linoleic acid metabolism, cilium movement, and natural killer (NK) cell activation. The subtype with high fatty acid metabolism had more severe pulmonary fibrosis than the other subtype. A reliable construction line chart model based on six diagnostic markers was constructed, and ABCA3 and CYP24A1 were identified as core diagnostic markers. Significant differences in immune cell infiltration were found between the two subtypes, and ABCA3 and CYP24A1 were closely related to NK cells.ConclusionFatty acid metabolism and the immune process that it mediates play an important role in the occurrence and development of IPF. The analysis of the role of FAMRGs in IPF may provide a new potential therapeutic target for IPF.

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Storage conditions of high‐fat diets affect pulmonary inflammation

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 2 publications

References 32 publications

Daily replacement of very high-fat diet stabilizes food intake and improves mouse welfare by ensuring food quality

Daily replacement of very high-fat diet stabilizes food intake and improves mouse welfare by ensuring food quality

Identification and immune characteristics of molecular subtypes related to fatty acid metabolism in idiopathic pulmonary fibrosis

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