Storage of Platelet Concentrates from Overnight-Stored Blood and Overnight-Stored Buffy Coat: In vitro Studies

Abstract

Platelet concentrates (PCs) were prepared from single buffy coats derived from fresh blood and from blood units stored overnight, as well as from buffy coats that were stored overnight. The platelet yield from overnight-stored buffy coats was similar to that of fresh blood or overnight-stored blood. PCs were stored at 20-24°C and on day 5 of storage, platelet aggregation with ADP was tested both at 37 and 25°C. Stored platelets aggregated better at 25°C than 37°C. The maximal aggregation (10 µA/ ADP) of stored platelets from overnight-stored buffy coats was 46±23% (n = 30), while that of stored platelets prepared either from fresh or overnight-stored blood was 27±21 % (n = 29) and 22± 15% (n = 29), respectively. Extracellular lactate dehydrogenase and ammonia levels, as well as elastase activity were similar in stored PCs of different origin. Our conclusion is that PCs prepared from overnight-stored buffy coat might also be suitable for storage and clinical use. In vivo studies are needed to confirm our findings.

Elimination and multiplication of bacteria during preparation and storage of buffy coat–derived platelet concentrates

“…For logistical reasons some institutions advocate overnight holding of buffy coats before the PCs are prepared. It was demonstrated that holding buffy coats at room temperature for up to 18 hours is not detrimental for PC functions 22‐25 . This raises the question whether with respect to product safety a short holding of the buffy coats at a lower temperature, for example, for 4 to 6 hours at 20°C, is preferable to a longer one at a higher temperature, for example, for 18 hours at 24°C.…”

Ambient overnight hold of whole blood prior to the manufacture of blood components

“…A number of studies have investigated platelet yields following ambient overnight hold of the WB prior to processing into BC. An early report found no difference in platelet yield from WB processed on the day of collection or after around 18‐h hold at ambient temperature (Racz & Baroti, 1995). However, Pietersz et al (1989) reported a tendency towards higher platelet yields in PC prepared from BC produced from WB following a 16‐ to 20‐h ambient hold compared with a 3‐h hold.…”

Platelet concentrates produced from whole blood using the Atreus processing system

“…A number of studies have investigated platelet yields following ambient overnight hold of the WB prior to processing into BC. An early report found no difference in platelet yield from WB processed on the day of collection or after around 18 h hold at ambient temperature [5]. However, Pietersz et al .…”