Storage of porcine pancreatic juice: effect on enzyme activities

Makkink, C.A.; Westerlaken, Lucette A. J. van der; Hartog, L.A. den; Baak, M.J. van; Huisman, J.

doi:10.1111/j.1439-0396.1990.tb00143.x

Cited by 4 publications

(3 citation statements)

References 9 publications

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“…This has been previously discussed and is outside the scope of this review but based on this work Makkink et al, 1990), samples in the current study will undergo one freeze-thaw cycle to reduce fluctuations in enzyme activity…”

Section: Slaughter Methodsmentioning

confidence: 99%

“…For the most part, photometric and pH-stat titration methods (Table 3) are used to determine enzyme activates. Makkink et al, 1990) pH Stat titration method…”

Section: Quantification Of Pancreatic Enzymesmentioning

confidence: 99%

“…Pancreatic digestive enzyme activity has been shown to be affected by storage conditions (Makkink et al, 1990). In order to preserve enzyme activity during storage, the use of general protease inhibitors is widely accepted (Carriere et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

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The impact of soluble dietary fibre on elements of digestive physiology

Pluschke¹

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Large scale epidemiological studies have shown that the consumption of whole cereal grains, fruits and vegetables is negatively associated with all-cause mortality and a decreased risk of developing metabolic syndrome conditions such as insulin resistance and cardiovascular diseases. Despite strong correlations between the consumption of whole cereal grains, fruits and vegetables and health, there is limited understanding of the underlying physiological mechanisms that explain the beneficial effects on health. Dietary fibre (DF) is the major non-digestible (in the small intestine) fraction of cereal grains, fruits and vegetables and has been associated with nutritional and health benefits such as an improved blood lipid profile, glycaemic control, diversity and activity of gut microbiota and alterations to rates of passage such as increased gastric retention time. 1968 unless a period of embargo has been approved by the Dean of the Graduate School.

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Section: Slaughter Methodsmentioning

confidence: 99%

“…For the most part, photometric and pH-stat titration methods (Table 3) are used to determine enzyme activates. Makkink et al, 1990) pH Stat titration method…”

Section: Quantification Of Pancreatic Enzymesmentioning

confidence: 99%

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The impact of soluble dietary fibre on elements of digestive physiology

Pluschke¹

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Electrophoretic separation of proteolytic enzymes in pancreatic juice collected with the pouch or catheter method

Gabert¹,

Jensen²,

Weström

et al. 1997

Int J Gastrointest Canc

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This qualitative enzyme assay indicated that a considerable amount of chymotrypsin C, anodal trypsin, chymotrypsins A and B, elastase II, and cathodal trypsin were present in samples of nonactivated pancreatic juice from PM pigs. In contrast, the only active enzymes identified in pancreatic juice from CM pigs were very small amounts of chymotrypsin A and elastase II. The amounts of chymotrypsin C and cathodal trypsin were lower in activated than in nonactivated pancreatic juice from PM pigs. However, there were increases in the amounts of the other enzymes when pancreatic juice from PM pigs was activated. As expected, the activation of pancreatic juice from CM pigs resulted in the measurement of very high amounts of all the proteolytic enzymes. The amounts of anodal trypsin, chymotrypsins A and B, and elastase II were higher in activated pancreatic juice from CM pigs than from PM pigs.

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