2019
DOI: 10.1016/j.fsigss.2019.09.068
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Storage of Second World War bone samples: Bone fragments versus bone powder

Abstract: Bone samples may yield low-quality and low-quantity DNA and duplicated analyses of different genetic markers have to be performed for identification of missing persons. Mostly no DNA extract is left after analyses and efficient storage of bones is needed to ensure the stability of the sample over time for retesting using new markers and new technologies. Usually not all of the bone powder prepared in grinder is used for extraction and rest can be stored for future analyses. After molecular genetic analyses of … Show more

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Cited by 5 publications
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“…Traditionally, bone and teeth samples are cleaned of debris, processed into smaller fragments, and cryogenically pulverised using liquid nitrogen and bone mills prior to DNA extraction. While these steps are time-consuming, attempts to bypass the pulverisation process through extracting DNA from bone fragments have generally resulted in lower DNA quantity [5,6]. Optimisation of the DNA extraction process provides the most potential to improve DNA profile success rates for bone and teeth.…”
Section: Introductionmentioning
confidence: 99%
“…Traditionally, bone and teeth samples are cleaned of debris, processed into smaller fragments, and cryogenically pulverised using liquid nitrogen and bone mills prior to DNA extraction. While these steps are time-consuming, attempts to bypass the pulverisation process through extracting DNA from bone fragments have generally resulted in lower DNA quantity [5,6]. Optimisation of the DNA extraction process provides the most potential to improve DNA profile success rates for bone and teeth.…”
Section: Introductionmentioning
confidence: 99%