1999
DOI: 10.1016/s0166-0934(99)00101-9
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Storage of viruses on filter paper for genetic analysis

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Cited by 22 publications
(15 citation statements)
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“…While the usefulness of filter paper blood samples for the measurement of MV-specific serum antibodies had been demonstrated before (3,12), recent publications have suggested that filter paper blood samples may also be suitable for RT-PCR analyses to diagnose certain virus infections (1,15). In the present study we show that blood samples spotted on filter paper are indeed suitable for use in MV-specific RT-PCR analysis.…”
supporting
confidence: 60%
“…While the usefulness of filter paper blood samples for the measurement of MV-specific serum antibodies had been demonstrated before (3,12), recent publications have suggested that filter paper blood samples may also be suitable for RT-PCR analyses to diagnose certain virus infections (1,15). In the present study we show that blood samples spotted on filter paper are indeed suitable for use in MV-specific RT-PCR analysis.…”
supporting
confidence: 60%
“…However, since no stored serum samples were available, we used smears of serially collected bone marrow samples, which are routinely obtained during treatment (from four to six bone marrow samples were available for each patient). This approach was chosen based on recent reports (1,23) of the potential of reverse transcription (RT)-PCR to detect viral RNA in dried blood spots. We thus attempted to investigate the presence of HCV RNA in glass slide preparations of bone marrow aspirates which had been serially collected from the five children involved in the outbreak and which had been stored in sterile sealed bags at room temperature.…”
Section: Methodsmentioning
confidence: 99%
“…Washing filter papers has reduced the amount of inhibitors, which in turn has allowed for conventional PCR on the filter disk (25). Alternatively, DNA samples have been extracted and/or purified from specimens on filters and used for PCR (3,5,18,27). Although dried blood spots can be used for CMV screening (2), the assay may not detect some cases of congenital infection, as blood specimens contain smaller amounts of CMV than urine specimens (4, 11; N. Inoue and S. Koyano, unpublished results).…”
mentioning
confidence: 99%