Storage of whole blood before separation: the effect of temperature on red cell 2,3 DPG and the accumulation of  lactate

Högman, Claes F.; Knutson, Folke; Lööf, H

doi:10.1046/j.1537-2995.1999.39050492.x

Cited by 50 publications

(47 citation statements)

References 25 publications

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“…In addition, the pH value gradually decreases due to lactate accumulation (Hogman et al, 1999). As the storage time-dependent pH variation of the model, we used the published time-course data of intracellular pH in commercially available cold-RC-MAP (Shiba et al, 1991).…”

Section: Construction Of the Metabolic Model Of Cold-map-stored Erythmentioning

confidence: 99%

In silico modeling and metabolome analysis of long-stored erythrocytes to improve blood storage methods

Nishino

Yachie‐Kinoshita

Hirayama

et al. 2009

Journal of Biotechnology

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Section: Construction Of the Metabolic Model Of Cold-map-stored Erythmentioning

confidence: 99%

In silico modeling and metabolome analysis of long-stored erythrocytes to improve blood storage methods

Nishino

Yachie‐Kinoshita

Hirayama

et al. 2009

Journal of Biotechnology

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“…Delayed sample analysis could result in haematological changes in the measured parameter, which could complicate the interpretation of the resulting data [4] . Blood testing is generally done on whole blood, plasma, or serum [5] . Whole blood is usually treated with anticoagulants to prevent them from clotting [6] .…”

Section: Introductionmentioning

confidence: 99%

Prolong Storage of Blood in EDTA Has an Effect on the Morphology and Osmotic Fragility of Erythrocytes

Antwi‐Baffour¹,

Quao²,

Kyeremeh³

et al. 2013

IJBSE

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Blood for various laboratory analyses are commonly kept in ethylenediamine tetra-acetic acid (EDTA). EDTA can however cause morphological and fragility changes in blood cells particularly erythrocytes (RBCs) if the storage is prolonged. This can affect erythrocytes viability and hence their analytical results. The timing between blood sampling and analysis is therefore very important in achieving reliable results. The objective of this study was to investigate the storage effects of EDTA on erythrocytes morphology and osmotic fragility over a period of 4 days. A total of twenty-four (24) consenting, apparently healthy blood donors who passed the pre-donation screening were recruited for the study. Blood samples were collected into EDTA tubes and analysed for changes in erythrocyte morphology and osmotic fragility in 24 hour interval over the four day period. On Day 1(control), sample analysis were done within four hours after collection, they were then stored refrigerated (4 -8 o C) and re-analysed from Day 2 to Day 4. Morphological changes observed in erythrocytes over time include echinocytosis, spherocytosis, sphero-echinocytosis and increase in rouleaux formation. Mean percentage haemolysis of erythrocytes increased from Day 1 to Day 4 (p>0.05). Again, the osmotic fragility curves of the RBCs exhibited a rightward shift suggestive of decrease in RBC membrane stabilization. Analysis of blood samples for haematological parameters should therefore be carried out as soon as possible, preferably within 4 hours after their collection to ensure clinically reliable results.

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“…4 The conductivity cell Stored RBCs were depleted of 2,3-DPG (Table 1) and they have greater oxygen affinity, and supply less oxygen to tissues [14]. The concentration of 2,3-DPG in the cell is regulated by an enzyme at high pH levels and because of the decreasing pH level during storage, 2,3-DPG could not be regulated [14]. Table 1 shows that 2,3-DPG mean value decreased to 19% of its initial value on the 21st day.…”

Section: Resultsmentioning

confidence: 99%

Physiological quality assessment of stored whole blood by means of electrical measurements

Ülgen

Sezdi

2007

Med Bio Eng Comput

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The physiological parameters of blood such as extracellular Na + , K + , Cl -, pH, 2,3-DPG and ATP and the complex electrical impedance were measured using whole blood samples from 31 male donors (21 donors form the training set and ten donors were used for testing), on the 0th, 10th and 21st day of blood bank storage. During storage, while the extracellular fluid resistance (R e ) and the intracellular fluid resistance (R i ) decreased progressively with time, the effective cell membrane capacitance (C m ) has increased. Blood bank storage resulted in a rise in K + and a fall in Na + , Cl -, pH, 2,3-DPG and ATP. Accordingly, all electrical parameters correlated with Na + , K + , Cl -, pH and ATP, at varying levels. By applying the multi-regression analysis, it was demonstrated that R i , R e and especially C m were appropriate for the assessment of Na + , K + , Cl -, pH and ATP until the 21st day of storage.

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Storage of whole blood before separation: the effect of temperature on red cell 2,3 DPG and the accumulation of lactate

Abstract: With current anticoagulants, storage of whole blood at temperatures of 25 to 30 degrees C before separation causes a great and rapid loss of 2,3 DPG and an accumulation of acid metabolites. In a hold of blood for >4 hours, rapid cooling is desirable to avoid initial loss of 2,3 DPG.

Cited by 50 publications

References 25 publications

In silico modeling and metabolome analysis of long-stored erythrocytes to improve blood storage methods

In silico modeling and metabolome analysis of long-stored erythrocytes to improve blood storage methods

Prolong Storage of Blood in EDTA Has an Effect on the Morphology and Osmotic Fragility of Erythrocytes

Physiological quality assessment of stored whole blood by means of electrical measurements

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