Storage proteins of the fall webworm, Hyphantria cunea drury

Abstract

Two storage proteins, storage protein-I (SPI) and storage protein-2 (SP2), were found in hemolymph and fat body during the development of Hyphantria cunea, the fall webworm. Both storage proteins show similiar quantitative changes during development in males and females; however, SPI is more abundant. The hemolymph of last instar larvae contains high concentrations of the storage proteins. However, following pupation, the storage proteins accumulate in fat bodies. SP1 peaks in the hemolymph of males and females late in last instar larvae (8-day-old 7th instar larvae).SP1 has a native molecular weight of 460,000 and consists of six identical subunits (Mr=76,700), while SP2 has a molecular weight of 450,000 and is composed of two different subunits (Mr=74,100 and 72,400). Both SP1 and SP2 are hexamers and are phosphorylated glycolipoproteins. The pl values of SP1 and SP2 were determined to be 5.70 and 5.50, respectively.Antibodies raised against SP1 react positively with vitellogenin and ovary extract, as well as with proteins in the hemolymph from last instar larvae and proteins in pupal fat bodies. Storage protein synthesis starts in fat bodies of a 4-day-old 7th instar larvae and in female peaks at 6-8 days of the 7th instar.

Two juvenile hormone suppressible storage proteins may play different roles inHyphantria cuneaDrury

“…Although several tissues have been identified as alternate expression sites of storage proteins, the types of tissues involved are not consistent among species. In general, the relative abundance of HcSP-2 in H. cunea was considerably less than that of HcSP-1 (Kim et al, 1989). Thus, HcSP-2 likely makes a lesser contribution to the storage system than does HcSP-1.…”

“…1). HcSP-1 and HcSP-2 reacted positively with PAS, Sudan Black B, and methyl green stains, indicating that they contain carbohydrates, lipids, and phosphate (Kim et al, 1989). A translational stop codon TAA was located at +2260 (HcSP-1) and +3242 (HcSP-2).…”

Purification and characterization of yolk protein‐2 from the fall webworm,Hyphantria cuneadrury

“…The SP-1 and LP present in the larval hemolymph of H. cunea reacted immunologically with ovarial extracts of the same species (Kim et al, 1989;Yun and Kim, 1993). However, YP2 did not react immunologically with SP-1 and LP (Fig.…”

“…In H. cunea, storage protein-1 (SP-1) and lipophorin (LP) are present in the hemolymph of last instar larvae (Kim et al, 1989;Yun and Kim, 1993). These proteins cross-reacted immunologically with ovarial extracts but not with YP2.…”

cDNA of an arylphorin‐type storage protein from Pieris rapae with parasitism inducible expression by the endoparasitoid wasp Pteromalus puparum

“…In most cases, the potential Nglycosylation sites are featured as NXT/S, while it was slightly different in PraAry, as NYSD. Kim et al (1989) reported that storage proteins can react positively to PAS, Sudan Black B, and methyl green stains, demonstrating that they contain carbohydrates, lipids, and phosphate. In the case of PraAry, carbohydrate and phosphorylation legends were present.…”