Stormwater loadings of antibiotic resistance genes in an urban stream

Garner, Emily; Benitez, Romina; Wagoner, Emily von; Sawyer, R.M.; Schaberg, Erin; Hession, W. Cully; Krometis, Leigh‐Anne; Badgley, Brian D.; Pruden, Amy

doi:10.1016/j.watres.2017.06.046

Cited by 109 publications

(51 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As event basis, CSO flow patterns are often affected by climate conditions. In tropical regions, where rainfall is often short and intense, CSO flows reach at a very high peak within half an hour, and end within an hour 27,28 , while CSO flows in temperate regions are rather smaller and longer to last for several hours or sometimes for days 16,17,23 . This suggests that CSOs in temperate regions could have more pollutant loads due to less dilution and longer duration.…”

Section: Discussionmentioning

confidence: 99%

“…However, there are a limited number of studies on ARB discharge from CSO. Garner et al 23 recently quantified antibiotic-resistant genes (ARG) at an urban stormwater outfall, and reported that a single CSO event could bring 1-to 3-log larger loadings of ARG than the dry-weather event. However, even though a single CSO event has a large discharge of ARB, the CSO events occur only occasionally.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Estimated discharge of antibiotic-resistant bacteria from combined sewer overflows of urban sewage system

et al. 2020

View full text Add to dashboard Cite

Yearly discharge of antibiotic-resistant bacteria (ARB) from combined sewer overflow (CSO) was estimated. The volume of CSO was estimated from operating data of the pumping station. In the target sewer catchment, 23% of the total of the volume of combined sewage was discharged untreated as CSO. Combined sewage contained 3-log larger E. coli than secondary treatment effluent although the abundance of antibiotic-resistant E. coli was not significantly different. In the target-combined sewer catchment, a yearly total of 4.8 × 10 16 CFU of E. coli was discharged from 6.1 × 10 6 m 3 of CSO, while 1.3 × 10 12 CFU of E. coli from 2.1 × 10 7 m 3 of effluent from the wastewater treatment plant (WWTP). This E. coli discharge was equivalent to 7.9 × 10 9 CFU/m 3 from CSO, and 6.2 × 10 4 CFU/m 3 from WWTP effluent. Consequently, a yearly total discharge of antibiotic-resistant E. coli from CSO was 3.7-log larger than the WWTP effluent. The small-flow CSO events, which had hourly flow rate smaller than five times of the average dryweather flow, accounted for 43% of the total CSO volume, but 79% of the total discharge of antibiotic-resistant E. coli due to a small dilution factor with stormwater and frequent discharge. Reduction of small-flow CSO events would be important for effective reduction of ARB discharge from CSO.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Estimated discharge of antibiotic-resistant bacteria from combined sewer overflows of urban sewage system

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In May and June, a large amount of rainfall brought pollution from the soil into the natural water bodies, which aggravated the pollution of the natural water bodies and increased the amount of ARGs [13]. However, a large amount of rainfall will also accelerate the river water flow and bring pollutants and ARGs into the downstream environment by the migration of water flow [38]. The ARGs in natural waters are highly susceptible to storm floods.…”

Section: Time and Spatial Distribution Of Argsmentioning

confidence: 99%

Distribution of Antibiotic Resistance Genes in Three Different Natural Water Bodies-A Lake, River and Sea

Su¹,

Li²,

Yang

et al. 2020

IJERPH

View full text Add to dashboard Cite

Currently, due to abuse in the use of human antibiotics and the weak regulatory control that the authorities have over sewage discharge and manure management, antibiotic resistance genes (ARGs) have become a new type of environmental pollutant. Three different natural water bodies (Poyang Lake, Haihe River and Qingdao No.1 Bathing Beach seawater) were sampled during the same periods to conduct a longitudinal comparison of distribution. The distribution and expression of 11 ARGs in 20 species were studied, and the correlations between the expression and the distribution of time and space of the ARGs in different water bodies were also analyzed. With the exception of ermA, blaNDM-1 and vanA, which were not detected in seawater, the other ARGs could be detected in all three water bodies. Tetracycline resistance genes (tetC, tetM and tetQ) in the seawater and Haihe River had even reached 100%, and sulfa ARGs (sul1 and sul2) in the seawater and Poyang Lake, as well as sul2 and sul3 in the Haihe River, had also reached 100%. The ARG pollution in Haihe River was much more serious, since 14 and 17 of 20 ARG species were significantly higher compared with seawater and Poyang Lake, respectively. Some ARGs also had a high absolute abundance. The absolute abundance of macrolide resistance genes (ermB) in seawater was as high as 8.61 × 107 copies/L, and the anti-tuberculosis resistant genes (rpoB and katG) in the Haihe River Basin were highly abundant at 1.32 × 106 copies/L and 1.06 × 107 copies/L, respectively. This indicates that ARGs have gradually become more diverse and extensive in natural water bodies. The results of a redundancy analysis (RDA) of the three water bodies showed that although each water body is affected by different factors in space and time, overall, the presence of AGRs is closely related to the production and life of human beings and the migration of animals.

show abstract

“…Independent distribution of Shigella phages from Shigella bacterial cells There are many reports concerning isolation of Shigella phages from sources such as municipal wastewater, drainage ponds, and surface runoff after regional occurrence of shigellosis, which could be due to the contact of Shigella -contaminated resource (such as the municipal wastewaters) with other water resources that usually are Shigella free (such as the runoff waters) [4,9,[45][46][47][48]. In the present study, a number of Shigella-infecting phages were isolated from a large freshwater resource, the Yangtze River, and in an area with no prevalence of Shigella.…”

Section: Discussionmentioning

confidence: 99%

Biodiversity of new lytic bacteriophages infecting Shigella spp. in fresh water environment

Barazandeh

Hedayatkhah

Mansoorianfar

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Bacteriophages, viruses that infects and replicates within prokaryotic cells are the most abundant life forms in the environment, yet the vast majority of them have not been properly reported or even discovered. Almost all reported bacteriophages infecting the Enterobacteriaceae family, with E. coli being the major subject of the study, have been isolated from wastewater, sewage, and effluent resources. In the present study we focused on the distribution and biodiversity of Shigella phages in an aquatic ecosystem. Results While no Shigella bacteria was recovered from the Yangtze River, three lytic phages were isolated from this ecosystem and were subjected to biological, morphological, and genomic characteristics. Comparative genomics and phylogenetic analyses demonstrated that vB _SflM_004 isolate belongs to Myoviridae family, Felixounavirus genus of Ounavirinae subfamily, vB_SdyM_006 was classified under the same family, however, it is suggested to be in a new genus under Tevenvirinae subfamily with some other related bacteriophages. vB_SsoS_008 phage belongs to the Siphoviridae family, Tunavirus genus, Tunavirinae subfamily. The phages did not harbor any genes involved in the lysogenic cycles, and showed a high temperature and pH stability. Conclusions It can be concluded that isolation of bacteriophages could be independent of their bacterial host presence in the isolation environment.

show abstract

Stormwater loadings of antibiotic resistance genes in an urban stream

Cited by 109 publications

References 84 publications

Estimated discharge of antibiotic-resistant bacteria from combined sewer overflows of urban sewage system

Estimated discharge of antibiotic-resistant bacteria from combined sewer overflows of urban sewage system

Distribution of Antibiotic Resistance Genes in Three Different Natural Water Bodies-A Lake, River and Sea

Biodiversity of new lytic bacteriophages infecting Shigella spp. in fresh water environment

Contact Info

Product

Resources

About