STR analysis of degraded DNA using a miniplex

Nastainczyk, M.; Schulz, Susanne; Kleiber, M.; Immel, Uta-Dorothee

doi:10.1016/j.fsigss.2009.08.107

Cited by 3 publications

(2 citation statements)

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“…Their high mutation rates and high heterozygosis make them ideal for accurately discerning relatives. Laboratory protocols to genotype this type of marker in highly degraded specimens have been developed (Butler et al, 2003;Nastainczyk et al, 2009;Vanek et al, 2009) and are suitable for samples recovered from archeological contexts. In palaeogenomic studies, another type of marker is employed, namely Single-Nucleotide Polymorphisms (SNPs).…”

Section: Computational Methods For Kinship Inferencementioning

confidence: 99%

“…When different kits for analysis of autosomal STRs are used on the same samples, the AmpFlSTR1MiniFiler PCR Amplification Kit shows a higher rate of amplification success in terms of typed loci and number of samples (Vanek et al, 2009). This kit is indeed specifically designed for degraded DNA, producing short amplicons (71-250 bp), also called miniSTRs (Butler et al, 2003;Nastainczyk et al, 2009), and remains the best strategy to obtain complete or almost complete autosomal profiles in ancient samples (Gamba et al, 2011).…”

Section: First Approaches To Kinship Analysismentioning

confidence: 99%

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Kinship Determination in Archeological Contexts Through DNA Analysis

et al. 2020

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Knowing kinship relations between individuals in archeological contexts is of great importance to understand social habits and structure in past human communities. Archeological and anthropological analyses of burial sites and skeletal remains often allow us to infer connections between individuals, but only genetic analysis can provide a sound determination of kinship. Several case studies are now available in the literature that show the potentiality and limitations of different methodological approaches based on ancient DNA (aDNA). Both experimental and computational strategies for kinship estimation on ancient samples are described in this review and we argue that, within a multidisciplinary approach, kinship inference contributes to the understanding of the biological and cultural patterns that characterized past societies.

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Section: Computational Methods For Kinship Inferencementioning

confidence: 99%