STR Data for the AmpFlSTR® Identifiler® Loci from the Three Main Ethnic Indigenous Population Groups (Iban, Bidayuh, and Melanau) in Sarawak, Malaysia

Suadi, Zaliha; Siew, Lau Cheng; Tie, Rita; Hui, Wee Boon; Asam, Anil; Thiew, Sim Hang; Boon, Lim Kong

doi:10.1111/j.1556-4029.2006.00344.x

Cited by 6 publications

(3 citation statements)

References 1 publication

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“…These include three Orang Asli groups (Semang, Senoi, and Proto-Malays), Malays (including Malay sub-ethnic groups), Chinese, and Indians. All groups are descendants of ancestrally- and genetically-unrelated ethnic groups [ 8 , 9 , 10 ]. Malays, Proto-Malays, and Malay sub-ethnic groups might be considered as exceptions, because they are ancestrally-related Austronesian speakers [ 11 , 12 , 13 , 14 ].…”

Section: Dna Population Data For Casework and Paternity Testing Stmentioning

confidence: 99%

“…The STR population datasets currently available for various population groups in Malaysia are listed in Table 3 . These datasets were mainly produced by researchers from academic institutions [ 8 , 9 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 ], and are not part of the RMP database. Mathematical probabilities calculated from these data do indicate that the STR loci tested are reliable for the identification of individuals in criminal cases and for kinship analysis associated with paternity disputes and immigration applications.…”

Section: Dna Population Data For Casework and Paternity Testing Stmentioning

confidence: 99%

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Autosomal STR Profiling and Databanking in Malaysia: Current Status and Future Prospects

Hakim

Khan²,

Lalung

et al. 2020

Genes

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Science and technology are extensively used in criminal investigation. From the mid- to late-1980s, one of the scientific discoveries that has had a particularly remarkable impact on this field has been the use of highly variable DNA sequence regions (minisatellites) in the human genome for individual identification. The technique was initially referred to as DNA fingerprinting, but is now more widely referred to as DNA profiling. Since then, many new developments have occurred within this area of science. These include the introduction of new genetic markers (microsatellites also known as short tandem repeats/STRs), the use of the polymerase chain reaction for target amplification, the development of DNA databases (databanking), and the advancement and/or improvement of genotyping protocols and technologies. In 2019, we described the progress of DNA profiling and DNA databanking in Malaysia for the first time. This report included information on DNA analysis regulations and legislation, STR genotyping protocols, database management, and accreditation status. Here, we provide an update on the performance of our DNA databank (numbers of DNA profiles and hits) plus the technical issues associated with correctly assigning the weight of evidence for DNA profiles in an ethnically diverse population, and the potential application of rapid DNA testing in the country. A total of 116,534 DNA profiles were obtained and stored in the Forensic DNA Databank of Malaysia (FDDM) by 2019, having increased from 70,570 in 2017. The number of hits increased by more than three-fold in just two years, where 17 and 69 hits between the DNA profiles stored in the FDDM and those from crime scenes, suspects, detainees, drug users, convicts, missing persons, or volunteers were recorded in 2017 and 2019, respectively. Forensic DNA analysis and databanking are thus progressing well in Malaysia and have already contributed to many criminal investigations. However, several other issues are discussed here, including the need for STR population data for uncharacterized population groups, and pilot trials for adopting rapid DNA profiling technology. These aspects should be considered by policy makers and law enforcement agencies in order to increase the reliability and efficiency of DNA profiling in criminal cases and in kinship analysis in Malaysia.

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Section: Dna Population Data For Casework and Paternity Testing Stmentioning

confidence: 99%

Section: Dna Population Data For Casework and Paternity Testing Stmentioning

confidence: 99%

Autosomal STR Profiling and Databanking in Malaysia: Current Status and Future Prospects

Hakim

Khan²,

Lalung

et al. 2020

Genes

View full text Add to dashboard Cite

show abstract

“…Final reaction volumes were 10 L each. The cycling program started with an initial 11-min denaturation step at 95°C, followed by 28 cycles of 1 min at 94°C, 1 min at 59°C, 1 min at 72°C, and the final extension step of 60 min at 60°C (Suadi et al, 2007). SCNT blastocysts were identified by LCN-STR profiling (Lucy et al, 2007).…”

Section: Culture Of In Vitro Fertilization (Ivf)-derived Embryosmentioning

confidence: 99%

Human Embryos Derived by Somatic Cell Nuclear Transfer Using an Alternative Enucleation Approach

Liu

Wang

et al. 2009

Cloning and Stem Cells

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Somatic cell nuclear transfer (SCNT) was used to generate patient-specific embryonic stem cells (ESCs) from blastocysts cloned by nuclear transfer (ntESCs). In this study, a total of 135 oocytes were obtained from 12 healthy donors (30-35 years). Human oocytes, obtained within 2 h following transvaginal aspiration, were enucleated using a Spindle Imaging System to position the spindle and chromosomes that lay on the metaphase plate, and a Zona Infrared Laser Optical System was used to open a single hole in the zona pellucida at the ~ 2 o'clock position. Human fibroblasts and lymphocytes were used to construct SCNT embryos. Nearly half (26 of 58) of the oocytes were fused after electrofusion and embryo development rates were 96.2% (two-cell, 25 of 26), 92.3% (four-cell, 24 of 26), 61.5% (eight-cell, 16 of 26), 34.6% (16-cell, 9 of 26), 26.9% (morula, 7 of 26), and 19.2% (blastocyst, 5 of 26), respectively, following incubation in improved G-series sequential medium. One cloned blastocyst was used for STR-DNA identification and genetic polymorphism analysis of mtDNA, and STR-DNA analysis of all cloned blastocysts indicated they were derived from SCNT. Quantitative analysis showed that mtDNA of cloned embryos reflected the change tendency of those observed in human IVF embryos. Our research provides an alternative enucleation approach for producing human SCNT-derived blastocysts, and may aid in providing a new method for human therapeutic cloning.

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Ascertaining the role of Taiwan as a source for the Austronesian expansion

Mirabal¹,

Cadenas

Garcia-Bertrand

et al. 2013

American J Phys Anthropol

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Taiwanese aborigines have been deemed the ancestors of Austronesian speakers which are currently distributed throughout two-thirds of the globe. As such, understanding their genetic distribution and diversity as well as their relationship to mainland Asian groups is important to consolidating the numerous models that have been proposed to explain the dispersal of Austronesian speaking peoples into Oceania. To better understand the role played by the aboriginal Taiwanese in this diaspora, we have analyzed a total of 451 individuals belonging to nine of the tribes currently residing in Taiwan, namely the Ami, Atayal, Bunun, Paiwan, Puyuma, Rukai, Saisiyat, Tsou, and the Yami from Orchid Island off the coast of Taiwan across 15 autosomal short tandem repeat loci. In addition, we have compared the genetic profiles of these tribes to populations from mainland China as well as to collections at key points throughout the Austronesian domain. While our results suggest that Daic populations from Southern China are the likely forefathers of the Taiwanese aborigines, populations within Taiwan show a greater genetic impact on groups at the extremes of the current domain than populations from Indonesia, Mainland, or Southeast Asia lending support to the "Out of Taiwan" hypothesis. We have also observed that specific Taiwanese aboriginal groups (Paiwan, Puyuma, and Saisiyat), and not all tribal populations, have highly influenced genetic distributions of Austronesian populations in the pacific and Madagascar suggesting either an asymmetric migration out of Taiwan or the loss of certain genetic signatures in some of the Taiwanese tribes due to endogamy, isolation, and/or drift.

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STR Data for the AmpFlSTR^® Identifiler^® Loci from the Three Main Ethnic Indigenous Population Groups (Iban, Bidayuh, and Melanau) in Sarawak, Malaysia

Abstract: POPULATION: Iban (n=195), Bidayuh (n=195), Melanau (n=128).

Cited by 6 publications

References 1 publication

Autosomal STR Profiling and Databanking in Malaysia: Current Status and Future Prospects

Autosomal STR Profiling and Databanking in Malaysia: Current Status and Future Prospects

Human Embryos Derived by Somatic Cell Nuclear Transfer Using an Alternative Enucleation Approach

Ascertaining the role of Taiwan as a source for the Austronesian expansion

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