Straightforward functionalization of breath figures: Simultaneous orthogonal host–guest and pH-responsive interfaces

León, Alberto Sanz de; Muñoz‐Bonilla, Alexandra; Gallardo, Alberto; Fernández‐Mayoralas, Alfonso; Bernard, Julien; Rodríguez‐Hernández, Juan

doi:10.1016/j.jcis.2015.06.039

Cited by 8 publications

(6 citation statements)

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“…In view of the potential use of these catalytic surfaces with other enzymes and taking advantage of the host–guest interactions to reuse these functional porous surfaces we evaluated the possibility to recover the immobilized HRP-Ada enzyme. For this purpose, a displacement of the immobilized HRP-Ada from the surface is attempted by means of an excess of free β-CD . Surfaces containing HRP-Ada were immersed in an aqueous solution of 6.0 mg/mL of β-CD in buffer phosphate pH 6.8 with 0.2% Triton X100 and 3 mg/mL albumin during 2 h at 25 °C.…”

Section: Results and Discussionmentioning

confidence: 99%

“…Polystyrene bearing side β-CD moieties has been prepared by facile standard radical copolymerization of styrene (S) and a monomer modified with cyclodextrin (SCD) at 10 wt % of SCD with respect to S. SCD is a styrenic monomer previously described in the literature . This P(S- co -SCD) copolymer was obtained using previously reported procedures . Example of recipe: 115 μL of S (104 mg), 10.4 mg of SCD and 2.5 mg of AIBN were dissolved in 1 mL of DMF:DMSO 1:1.…”

Section: Methodsmentioning

confidence: 99%

“…20 This P(S-co-SCD) copolymer was obtained using previously reported procedures. 21 Example of recipe: 115 μL of S (104 mg), 10.4 mg of SCD and 2.5 mg of AIBN were dissolved in 1 mL of DMF:DMSO 1:1. The total concentration of comonomers was 1 mol/L and the initiator concentration was 0.015 mol/L.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…Synthesis of N-Adamantane-1-carbonyl Fluoresceinamine (Ada-FAm). According to our previously reported procedure, 21 to a solution of adamantane-1-carbonyl chloride (12 mg, 0.06 mmol) in dry dichloromethane (0.3 mL) was added a mixture of fluoresceinamine (15 mg, 0.043 mmol), trimethylamine (9.3 μL, 0.06 mmol), and 4dimethylpyridine (1 mg) in dry dichloroethane (0.3 mL). The reaction mixture was stirred for 30 min at 0 °C and for 3 h at room temperature.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Microfluidic Reactors Based on Rechargeable Catalytic Porous Supports: Heterogeneous Enzymatic Catalysis via Reversible Host–Guest Interactions

León

Vargas-Alfredo²,

Gallardo³

et al. 2017

ACS Appl. Mater. Interfaces

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We report on the fabrication of a microfluidic device in which the reservoir contains a porous surface with enzymatic catalytic activity provided by the reversible immobilization of horseradish peroxidase onto micrometer size pores. The porous functional reservoir was obtained by the Breath Figures approach by casting in a moist environment a solution containing a mixture of high molecular weight polystyrene (HPS) and a poly(styrene-co-cyclodextrin based styrene) (P(S-co-SCD)) statistical copolymer. The pores enriched in CD were employed to immobilize horseradish peroxidase (previously modified with adamantane) by host-guest interactions (HRP-Ada). These surfaces exhibit catalytic activity that remains stable during several reaction cycles. Moreover, the porous platforms could be recovered by using free water-soluble β-CD with detergents. An excess of β-CD/TritonX100 in solution disrupts the interactions between HRP-Ada and the CD-modified substrate thus allowing us to recover the employed enzyme and reuse the platform.

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Section: Results and Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%

See 2 more Smart Citations

Microfluidic Reactors Based on Rechargeable Catalytic Porous Supports: Heterogeneous Enzymatic Catalysis via Reversible Host–Guest Interactions

León

Vargas-Alfredo²,

Gallardo³

et al. 2017

ACS Appl. Mater. Interfaces

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“…7 applied for many applications, such as adhesion-selective membranes, 12 cell culture scaffolds, 13 micro-containers, 14,15 biosensors 16,17 and micro-reactors, 17 and stimuli-responsive substance-releasing materials. 18,19 The surface morphology of honeycomb-patterned films, that is, the size and regularity of the pores, is important for all of the above applications. High regularity of pore dimensions is important for many applications, while more specifically the size of the pores affects the volume and surface area involved in interaction with target substances.…”

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confidence: 99%

The influence of casting parameters on the surface morphology of PS‐b‐P4VP honeycomb films

Chen

Blakey

Whittaker

2016

J. Polym. Sci. Part A: Polym. Chem.

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The “breath figures” method provides an efficient and cost‐effective method to produce highly ordered honeycomb patterns in polymeric films at micrometer and sub‐micrometer dimensions. The size and regularity of the pores can be adjusted through a series of physical and chemical parameters. In this study, amphiphilic diblock copolymers, polystyrene‐block‐poly(4‐vinyl pyridine) (PS‐b‐P4VP) with different lengths of P4VP, were synthesized through Reversible Addition‐Fragmentation Chain Transfer polymerization. The honeycomb‐patterned films were prepared from these well‐defined polymers through the dynamic breath figures method. A series of physical parameters including solution concentration, flow rate, humidity of the flow, and the humidity of the casting environment, were delicately adjusted to systematically investigate their effects on the morphology of the films. These studies identified four key factors which were found to influence the formation of the pattern. No obvious effect was found on the pore size by changing the length of P4VP block. The result provides clear direction on the fabrication of PS‐b‐P4VP honeycomb‐patterned films and more broadly contributes a deeper understanding of the processes involved in the formation of honeycomb patterns. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016, 54, 3721–3732

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Designing 3D Biological Surfaces via the Breath‐Figure Method

Chen

Gao

Jing

et al. 2018

Adv Healthcare Materials

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The fabrication of biointerfaces that mimic cellular physiological environments is critical to understanding cell behaviors in vitro and for the design of tissue engineering. Breath figure is a self-assemble method that uses water droplets condensed from moisture as template and ends up with a highly ordered hexagonal pore array; this approach is used to fabricate various biological substrates. This progress report provides an overview of strategies to achieve topographical modifications and chemical-patterned arrays, such as modulation of the pore size, shape and selective decoration of the honeycomb holes. Using recent results in the biological fields, potential future applications and developments of honeycomb structures are commented upon.

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Straightforward functionalization of breath figures: Simultaneous orthogonal host–guest and pH-responsive interfaces

Cited by 8 publications

References 50 publications

Microfluidic Reactors Based on Rechargeable Catalytic Porous Supports: Heterogeneous Enzymatic Catalysis via Reversible Host–Guest Interactions

Microfluidic Reactors Based on Rechargeable Catalytic Porous Supports: Heterogeneous Enzymatic Catalysis via Reversible Host–Guest Interactions

The influence of casting parameters on the surface morphology of PS‐b‐P4VP honeycomb films

Designing 3D Biological Surfaces via the Breath‐Figure Method

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