Strain and serovar variants of Salmonella enterica exhibit diverse tolerance to food chain-related stress

Pye, Hannah; Thilliez, Gaёtan; Acton, Luke; Kolenda, Rafał; Al-Khanaq, Haider; Grove, Stephen; Kingsley, Robert A.

doi:10.1016/j.fm.2023.104237

Cited by 14 publications

(10 citation statements)

References 48 publications

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“…Notably, even very closely related Salmonella strains exhibited diversity in sensitivity to SPLA phages. For example, S. Typhimurium strains S04698-09 and A53 were both part of the monophasic S. Typhimurium ST34 epidemic clade that emerged in the last three decades (32,33), yet these strains exhibited distinct differences in susceptibility to at least five SPLA phages. Furthermore, a S. Typhimurium strain of ST4/74 that was genetically modified to remove prophage elements from its genome exhibited a moderate decrease in sensitivity to several SPLA phages.…”

Section: Resultsmentioning

confidence: 99%

“…Typhimurium strains S04698-09 and A53 were both part of the monophasic S . Typhimurium ST34 epidemic clade that emerged in the last three decades (32, 33), yet these strains exhibited distinct differences in susceptibility to at least five SPLA phages. Furthermore, a S .…”

Section: Resultsmentioning

confidence: 99%

“…A similar level of variation in virulence of SPLA phages for eleven strains of S. Typhimurium to that for representative strains of diverse serovars was observed. This was perhaps surprising since S. Typhimurium exhibits less than 0.1% nucleotide sequence divergence in their genome (39), compared with up to 2% nucleotide sequence divergence for strains of S. enterica (32). S. Typhimurium accounts for almost one quarter of all Salmonella isolated from humans and 78% of pig isolates in 2020 in the UK (38), this serovar is of particular interest for interventions.…”

Section: Discussionmentioning

confidence: 99%

“…This was perhaps surprising since S . Typhimurium exhibits less than 0.1% nucleotide sequence divergence in their genome (39), compared with up to 2% nucleotide sequence divergence for strains of S. enterica (32). S .…”

Section: Discussionmentioning

confidence: 99%

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Collateral sensitivity increases the efficacy of a rationally designed bacteriophage combination to controlSalmonella enterica

Acton,

Pye,

Thilliez

et al. 2023

Preprint

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The ability of virulent bacteriophages to lyse bacteria influences bacterial evolution, fitness, and population structure. Knowledge of both host susceptibility and resistance factors is crucial for the successful application of bacteriophages as biological control agents in clinical therapy, food processing and agriculture. In this study, we isolated twelve bacteriophages termed SPLA phage which infect the foodborne pathogenSalmonella enterica. To determine phage host range, a diverse collection ofEnterobacteriaceaeandSalmonella entericawere used and genes involved in infection by six SPLA phages were identified usingSalmonellaTyphimurium strain ST4/74. Candidate host receptors included lipopolysaccharide, cellulose and BtuB. Lipopolysaccharide was identified as susceptibility factor for phage SPLA1a and mutations in LPS biosynthesis genes spontaneously emerged during culture withS. Typhimurium. Conversely, LPS was a resistance factor for phage SPLA5b that suggested that emergence of LPS mutations in culture with SPLA1a may represent a case of collateral sensitivity to SPLA5b. We show that combination therapy with SPLA1a and SPLA5b was more successful in limiting the emergence of phage resistance compared to monotherapy. Identification of host susceptibility and resistance genes and understanding infection dynamics is critical step in rationale design of phage cocktails against specific bacterial pathogens.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Collateral sensitivity increases the efficacy of a rationally designed bacteriophage combination to controlSalmonella enterica

Acton,

Pye,

Thilliez

et al. 2023

Preprint

Self Cite

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“…Under these circumstances, the high stress tolerance described for S . 1 ,4,[5],12:i:- among Salmonella serovars 30 and the efficient colonization and survival abilities displayed above its parent S . Typhimurium strain 31 , could account for the higher prevalence of this serovar at the end of the pig production chain (i.e., fresh sausages and, particularly DFS).…”

Section: Discussionmentioning

confidence: 99%

Genomic insights of Salmonella isolated from dry fermented sausage production chains in Spain and France

Ferrer-Bustins,

Yvon,

Martín

et al. 2024

Sci Rep

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The presence of Salmonella in dry fermented sausages is source of recalls and outbreaks. The genomic diversity of 173 Salmonella isolates from the dry fermented sausage production chains (pig carcasses, pork, and sausages) from France and Spain were investigated through their core phylogenomic relationships and accessory genome profiles. Ten different serovars and thirteen sequence type profiles were identified. The most frequent serovar from sausages was the monophasic variant of S. Typhimurium (1,4,[5],12:i:-, 72%) while S. Derby was in pig carcasses (51%). Phylogenomic clusters found in S. 1,4,[5],12:i:-, S. Derby, S. Rissen and S. Typhimurium serovars identified closely related isolates, with less than 10 alleles and 20 SNPs of difference, displaying Salmonella persistence along the pork production chain. Most of the S. 1,4,[5],12:i:- contained the Salmonella genomic island-4 (SGI-4), Tn21 and IncFIB plasmid. More than half of S. Derby strains contained the SGI-1 and Tn7. S. 1,4,[5],12:i:- genomes carried the most multidrug resistance genes (91% of the strains), whereas extended-spectrum β-lactamase genes were found in Typhimurium and Derby serovars. Salmonella monitoring and characterization in the pork production chains, specially S. 1,4,[5],12:i:- serovar, is of special importance due to its multidrug resistance capacity and persistence in dry fermented sausages.

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Predicting the impact of temperature and relative humidity on Salmonella growth and survival in sliced chard, broccoli and red cabbage

Alves,

Alvarenga,

Tavares da Silva

et al. 2024

Food Microbiology

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Strain and serovar variants of Salmonella enterica exhibit diverse tolerance to food chain-related stress

Cited by 14 publications

References 48 publications

Collateral sensitivity increases the efficacy of a rationally designed bacteriophage combination to controlSalmonella enterica

Collateral sensitivity increases the efficacy of a rationally designed bacteriophage combination to controlSalmonella enterica

Genomic insights of Salmonella isolated from dry fermented sausage production chains in Spain and France

Predicting the impact of temperature and relative humidity on Salmonella growth and survival in sliced chard, broccoli and red cabbage

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