Strain Differences in Histamine Release from Peritoneal Mast Cells in Rats

Sugimoto, Yukihiko; Ohishi, Hanako; Toyota, T; Kamei, Chiaki

doi:10.1016/s0306-3623(98)00063-9

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…The relatively high concentrations of antigen (1000 μg ml −1 ) required to cause histamine release from sensitized RPMC may reflect the strain of rat used in these experiments. RPMC from Wistar rats, the strain used in the present study, have been demonstrated to express less IgE per cell than RPMC obtained from Brown Norway rats (Sugimoto et al ., 1998), and therefore are consequently less reactive to challenge by antigen. These findings may suggest that intracellular ROS generation by RPMC is more readily detectable than histamine release.…”

Section: Discussionmentioning

confidence: 78%

Reactive oxygen species generation and histamine release by activated mast cells: modulation by nitric oxide synthase inhibition

Brooks

Whelan

Purcell

1999

British J Pharmacology

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1 We have examined the generation of intracellular reactive oxygen species (ROS) and release of histamine by rat peritoneal mast cells (RPMC) in response to stimulation with antigen (ovalbumin), compound 48/80, nerve growth factor (NGF) and substance P (SP). 2 We have also examined the eects of the non-speci®c nitric oxide synthase inhibitor, L-NAME (100 mM) upon the release of histamine and generation of intracellular ROS in response to the named secretagogues. 3 Ovalbumin (100 ± 1000 mg ml 71 ), compound 48/80 (0.1 ± 100 mg ml 71 ), NGF (0.1 ± 100 mg ml 71 ), and SP (5 ± 50 mM), caused a concentration-dependent release of histamine from RPMC. 4 Ovalbumin (1 ng ml 71 ± 0.1 mg ml 71), compound 48/80 (1 ± 100 mg ml 71 ), NGF (1 pg ml 71 ± 1 mg ml 71 ), and SP (0.005 ± 50 mM) caused a concentration-dependent generation of intracellular ROS by RPMC. 5 Pre-incubation of RPMC with L-NAME (100 mM) caused a signi®cant enhancement of both histamine release and intracellular ROS from RPMC in response to ovalbumin, compound 48/80, NGF and SP. 6 Our data demonstrate that NGF, SP and ovalbumin are capable of causing intracellular ROS generation by RPMC at lower concentrations than those causing signi®cant histamine release and we speculate that this may contribute to the activation of cytokine production. 7 The data also show that NO modulates histamine release, and ROS generation in response to the secretagogues used. This may have signi®cance in pathologies where NO synthesis is decreased, leading to an increased activation of mast cells.

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Section: Discussionmentioning

confidence: 78%

Reactive oxygen species generation and histamine release by activated mast cells: modulation by nitric oxide synthase inhibition

Brooks

Whelan

Purcell

1999

British J Pharmacology

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“…The mechanism for this differential sensitivity to DSCG between SD and WKY rats remains uninvestigated, but may be due to strain-related differences in the response to compound 48/80 [48]. Moreover, the inhibitory effects of DSCG can be enhanced by additional factors, for example alterations in cAMP or sympathetic activity [49], which may be relevant in our study, given WKY tissues displayed an increased Isc response to the cAMP activator, forskolin, though this did not reach significance.…”

Section: Discussionmentioning

confidence: 99%

Disodium Cromoglycate Reverses Colonic Visceral Hypersensitivity and Influences Colonic Ion Transport in a Stress-Sensitive Rat Strain

et al. 2013

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The interface between psychiatry and stress-related gastrointestinal disorders (GI), such as irritable bowel syndrome (IBS), is well established, with anxiety and depression the most frequently occurring comorbid conditions. Moreover, stress-sensitive Wistar Kyoto (WKY) rats, which display anxiety- and depressive-like behaviors, exhibit GI disturbances akin to those observed in stress-related GI disorders. Additionally, there is mounting preclinical and clinical evidence implicating mast cells as significant contributors to the development of abdominal visceral pain in IBS. In this study we examined the effects of the rat connective tissue mast cell (CTMC) stabiliser, disodium cromoglycate (DSCG) on visceral hypersensitivity and colonic ion transport, and examined both colonic and peritoneal mast cells from stress-sensitive WKY rats. DSCG significantly decreased abdominal pain behaviors induced by colorectal distension in WKY animals independent of a reduction in colonic rat mast cell mediator release. We further demonstrated that mast cell-stimulated colonic ion transport was sensitive to inhibition by the mast cell stabiliser DSCG, an effect only observed in stress-sensitive rats. Moreover, CTMC-like mast cells were significantly increased in the colonic submucosa of WKY animals, and we observed a significant increase in the proportion of intermediate, or immature, peritoneal mast cells relative to control animals. Collectively our data further support a role for mast cells in the pathogenesis of stress-related GI disorders.

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“…Η ετερογένεια των μαστοκυττάρων, ή αλλιώς ονομαζόμενων σιτευτικών κυττάρων, μεταξύ διαφορετικών ειδών αλλά και μεταξύ διαφορετικών ιστών του ίδιου οργανισμού αφορά φαινοτυπικά, βιοχημικά, λειτουργικά και φαρμακολογικά χαρακτηριστικά (Barrett et al 1983, Barrett and Metcalfe 1985, Benyon et al 1986, Undem et al 1986, Ennis et al 1987, Ottosson and Edvinsson 1997, Sugimoto et al 1998, Amano et al 2000, Singh et al 2001.…”

Section: ο ρόλος των μαστοκυττάρων στις αντιδράσεις υπερευαισθησίαςunclassified

Φαρμακολογική Μελέτη Του Ρόλου Του Μονοξειδίου Του Αζώτου (NO) Ως Μεσολαβητή Στην Παθογένεια Των Αντιδράσεων Υπερευαισθησίας Στον Οφθαλμό

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Strain Differences in Histamine Release from Peritoneal Mast Cells in Rats

Cited by 5 publications

References 18 publications

Reactive oxygen species generation and histamine release by activated mast cells: modulation by nitric oxide synthase inhibition

Reactive oxygen species generation and histamine release by activated mast cells: modulation by nitric oxide synthase inhibition

Disodium Cromoglycate Reverses Colonic Visceral Hypersensitivity and Influences Colonic Ion Transport in a Stress-Sensitive Rat Strain

Φαρμακολογική Μελέτη Του Ρόλου Του Μονοξειδίου Του Αζώτου (NO) Ως Μεσολαβητή Στην Παθογένεια Των Αντιδράσεων Υπερευαισθησίας Στον Οφθαλμό

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