Strain improvement of Aspergillus niger for the enhanced production of asperenone

Abstract

The enhancement of production of asperenone ( Fig. 1), an inhibitor of lipoxygenase and human platelet aggregation from Aspergillus niger CFTRI 1105, was achieved by UV and nitrous acid mutagenesis. Nitrous acid mutants exhibited increased inhibitor production when compared with UV irradiated mutants. I N 41 a fi rst-generation nitrous acid mutant produced 5.1 fold increased asperenone over parent strain. Mutant II N 31 obtained by second-generation nitrous acid treatment produced 60.3 mg asperenone/g biomass, which was 131 fold increase when compared to fi rst generated mutant I N 41 and 670 fold increase over the parent strain. This mutant was stable for several generations on production medium.

Safety of the fungal workhorses of industrial biotechnology: update on the mycotoxin and secondary metabolite potential of Aspergillus niger, Aspergillus oryzae, and Trichoderma reesei

“…The terpenes asperenones, asperyellones, and asperrubrols are carotenoid-like secondary metabolites. Asperenone is a human platelet aggregation inhibitor, and a strain of A. niger has been optimized for higher production of this bioactive compound (Chidananda et al 2008 ).…”

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“…The induction of modulation was carried out by exposure to UV germicidal lamp (256 nm) kept at a distance of 40 cm for time intervals ranging from 2-14 min, in a total of 56 minutes. A modification of [Chidananda et al 2008] [7] was applied. After irradiation, the plates were wrapped in aluminum foil and kept overnight at 4°C.…”

Aspergillus niger as a Secondary Metabolite Factory

“…Citric acid (118) and itaconic acid (119) have been large-scale products in food and pharmaceutical industry for decades (Andersen et al, 2011;Li et al, 2012). Some other valuable chemicals with low molecular weight are also produced by A. niger, such as 2-phenylethanol (128) (Etschmann et al, 2015), p-hydroxyphenylacetic acid (129) (Happi et al, 2015), gallic acid (130) (Saeed et al, 2020), benzoic acid derivative (131) (Zabala et al, 2012), and asperyellone (147) (Jefferson, 1967;Chidananda et al, 2008). In comparison with 119, the biological activity of hexylitaconic acid (120) dramatically attenuated (Varoglu et al, 1997;Varoglu and Crews, 2000).…”

“…Asperyellone ( 147) was the common product of strains NRRL-3 and CFTRI 1105 (Jefferson, 1967;Chidananda et al, 2008) and exhibited inhibitory effect on lipoxygenase and human platelet aggregation (Rao et al, 2002), UVB protection (Santhakumaran et al, 2019), and antifungal activity (Ayer et al, 1996). In addition to γ-pyridone (86), five highly oxygenated pyranoquinones (86, 149, and 151-154) were detected in SMs of strain T1 by activation of the aza gene cluster (Zabala et al, 2012) (Figure 8).…”