Strain improvement of industrially important microorganisms based on resistance to toxic metabolites and abiotic stress

Fiedurek, J.; Trytek, Mariusz; Szczodrak, Janusz

doi:10.1002/jobm.201600710

Cited by 20 publications

(5 citation statements)

References 178 publications

(183 reference statements)

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“…To deal with toxic solutes and survive under stressful conditions, microbes have evolved a multitude of adaptive networks [110]. It has been shown that halophile-produced enzymes can be important biological substances, such as phytohormones and exopolysaccharides, which are important in plant-microbiome interactions [111].…”

Section: Microbial Bioremediation Under Abiotic Stress Conditionsmentioning

confidence: 99%

Recent Trends in Microbial Approaches for Soil Desalination

2022

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Soil salinization has become a major problem for agriculture worldwide, especially because this phenomenon is continuously expanding in different regions of the world. Salinity is a complex mechanism, and in the soil ecosystem, it affects both microorganisms and plants, some of which have developed efficient strategies to alleviate salt stress conditions. Currently, various methods can be used to reduce the negative effects of this problem. However, the use of biological methods, such as plant-growth-promoting bacteria (PGPB), phytoremediation, and amendment, seems to be very advantageous and promising as a remedy for sustainable and ecological agriculture. Other approaches aim to combine different techniques, as well as the utilization of genetic engineering methods. These techniques alone or combined can effectively contribute to the development of sustainable and eco-friendly agriculture.

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Section: Microbial Bioremediation Under Abiotic Stress Conditionsmentioning

confidence: 99%

Recent Trends in Microbial Approaches for Soil Desalination

2022

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“…Enhancing biosynthesis pathways that are mediated by terpenoid intermediates is limited because of their relative toxicity at high concentrations. Alternatively, mutagenesis is a classic strategy that is widely used to improve microbial industrial-candidates (Fiedurek, Trytek, & Szczodrak, 2017). In the present study, nonionizing radiation was used to activate the paclitaxel productivity in the protoplasts of Acremonium sp.…”

Section: Stimulating the Paclitaxel Production By Two Strategiesmentioning

confidence: 99%

Elicitors stimulate paclitaxel production by endophytic fungi isolated from ecologically altered Taxus baccata

El-Bialy

El-Bastawisy

2020

Journal of Radiation Research and Applied Sciences

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The commercial production of paclitaxel; the most potent anti-cancer drug, is based on its extraction from Taxus spp (yews) or microbial fermentation from yews' endophytes. The present study investigated the capability of local endophytes that have been isolated from ecologically altered Taxus baccata (host tree) to produce this diterpenoid secondary metabolite and improve its production and stability. Results revealed the ability of three endophytes; Acremonium, Colletotrichum and Fusarium spp, to produce paclitaxel up to 116.19 μg l −1. The challenge of their production instability away from their host is overcome by two strategies; the addition of unusual elicitor (0.0001% crushed bark of T. baccata) and induction of mutagenesis using ultraviolet irradiation which stimulated the production to higher levels (reach four folds for parent strain Acremonium sp. MK). Produced paclitaxel has a strong cytotoxic effect on human breast cancer cell line (MCF-7). The molecular analysis of the endophyte; Acremonium sp. showed the presence of BAPT gene that responsible for paclitaxel production and encoding C-13 phenylpropanoid side chain-acetyl coenzyme A acetyltransferase. Therefore, the selected endophyte has an individual metabolic system that can be activated in the absence of the host or changing its ecology.

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“…In contrast to genetic engineering methods, classical mutagenesis facilitates obtaining highly efficient mutants in an easy and rapid way without specialized knowledge about microbe genomes. To obtain the required mutants, experimenters have enriched cell populations by culturing them in special environmental conditions, toxic to most cell types but less toxic or non-toxic to a desired minority of cells [24][25][26][27][28]. Induced mutation has been successfully used in a number of microbial processes (other than biotransformation) employed in the production of useful end products [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Mutagenesis and Adaptation of the Psychrotrophic Fungus Chrysosporium pannorum A-1 as a Method for Improving β-pinene Bioconversion

et al. 2020

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Mutagenesis and adaptation of the psychrotrophic fungus Chrysosporium pannorum A-1 to the toxic substrate β-pinene were used to obtain a biocatalyst with increased resistance to this terpene and improved bioconversion properties. Mutants of the parental strain were induced with UV light and N-methyl-N′-nitro-N-nitrosoguanidine. Mutants resistant to β-pinene were isolated using agar plates with a linear gradient of substrate concentrations. Active mutants were selected based on their general metabolic activity (GMA) expressed as oxygen consumption rate. Compared to the parental strain, the most active mutant showed an enhanced biotransformation ability to convert β-pinene to trans-pinocarveol (315 mg per g of dry mycelium), a 4.3-fold greater biocatalytic activity, and a higher resistance to H2O2-induced oxidative stress. Biotransformation using adapted mutants yielded twice as much trans-pinocarveol as the reaction catalyzed by non-adapted mutants. The results indicate that mutagenesis and adaptation of C. pannorum A-1 is an effective method of enhancing β-bioconversion of terpenes.

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Strain improvement of industrially important microorganisms based on resistance to toxic metabolites and abiotic stress

Cited by 20 publications

References 178 publications

Recent Trends in Microbial Approaches for Soil Desalination

Recent Trends in Microbial Approaches for Soil Desalination

Elicitors stimulate paclitaxel production by endophytic fungi isolated from ecologically altered Taxus baccata

Mutagenesis and Adaptation of the Psychrotrophic Fungus Chrysosporium pannorum A-1 as a Method for Improving β-pinene Bioconversion

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