Strain improvement of Serratia marcescens ECU1010 and medium cost reduction for economic production of lipase

Abstract

Industrial strain improvement plays a central role in the commercial development of microbial fermentation processes. The strain of Serratia marcescens ECU1010, a wild-type lipase-producer capable of stereospecific synthesis of a Diltiazem precursor, was subjected to physical mutation involving treatment by UV-irradiation for 30 s. A mutant strain, no. UV-01, showed enhanced lipase production, but lost the capability of producing red pigment (prodigiosin). The variant strain UV-01 had a 2.3-fold higher activity than the wild type and was stable in its enzyme production for ten serial transfers. For reduction of the fermentation medium cost, dried powder of corn steep liquor was used as an inexpensive substitute for beef extract in the medium. Dextrin as an organic carbon source and Tween-80 as an important element were further optimized, respectively. The high primary biodegradation of the Tween-80 by S. marcescens ECU1010 and its variant demonstrated their potential ability of degrading alkyl polyethoxylates to remove harmful nonionic surfactants from polluted effluents and streams. The optimal cultivation time for lipase biosynthesis was 24 h. These optimized compositions resulted in an economic production of lipase by S. marcescens ECU1010 var. UV-01, with a dramatically reduced cost (1/8-1/7 of the initial one) which is more suitable for industrial application.

Enhanced Production of Extracellular Alkaline Lipase by an Improved Strain of <i>Pseudomonas aeruginosa</i> MTCC 10,055

“…Zhao et al (2010) reported 2.3-fold increase in the lipase from a mutant strain of Serratia marcescens as compared to wild type strain after exposure to Ultra Violet (UV). Gaoa et al (2000), also reported 3.25-fold increase in lipase yield by mutant of Pseudomonas upon exposure to UV and Nmethyl-N'-nitro-N-nitrosogunidine (NTG).…”

“…Improvement of the commercial applicability of microbial strains has been practiced for centuries (Parekh et al, 2000). Industrial strain improvement plays a central role in the commercial development of microbial fermentation processes (Zhao et al, 2010). Meanwhile, strain improvement of the enzyme producer offers the greatest opportunity for cost effective production without significant capital outlay (Stanbury et al, 1995).…”

“…Meanwhile, strain improvement of the enzyme producer offers the greatest opportunity for cost effective production without significant capital outlay (Stanbury et al, 1995). This is achieved when a selected strain can synthesize a higher proportion of the product using the minimal quantity of raw materials (Zhao et al, 2010).…”

Pilot-Scale Production of Lipase Using Palm Oil Mill Effluent as a Basal Medium and Its Immobilization by Selected Materials

“…Among the carrier matrices studied, porous inorganic materials with high specific surface areas and large porous volumes were considered for enzyme immobilization 33,34 .…”

High‐level soluble expression of Serratia marcescensH30 lipase in Escherichia coli

“…The strain S. marcescens ECU1010 was further subjected to ultraviolet irradiation. A mutant strain (UV‐01) has only given a 2.3‐fold increase in lipase activity compared with a wild‐type strain . SmL genes from different S. marcescens have also been cloned and expressed in recent years.…”