Strain measurements have a significant role in evaluating the condition of various structural types and have become an essential component in the area of structural health monitoring. However, there are some limitations in the current means of strain measurement, and this study aims to improve these methods. We have designed a novel strain measurement method based on template matching algorithms and microscopic vision techniques, developed a new sliding strain sensor, and paired it with a new microscope to realize strain measurement. The method has the function of remote wireless acquisition with a cell phone, which is more widely applicable. In the laboratory performance testing, the zero drift of the sensor is mainly concentrated in the fluctuation range of ±2 με, and the effective range reaches nearly 40,000 με. In the comparison experiments with the linear variable differential transformer, the maximum error of the static loading is only 5 με, and the maximum error rate of the dynamic loading is less than 1%, which proves that it has a relatively high accuracy. Finally, the short-term real-time monitoring of the local structure of the footbridge was accomplished, and the strain changes on the surface of the structure were captured instantly, stably, and efficiently in the actual measurements. The proposed strain measurement system has the advantages of high accuracy, a low cost, convenient measurement, and wide applicability, and it provides a novel alternative means for strain measurement in the field of structural health monitoring.