Strain selection and scale-up fermentation for FR901379 acylase production by Streptomyces sp. no. 6907

Ueda, Satoshi; Kinoshita, Masayoshi; Tanaka, Fumihiro; Tsuboi, Masaru; Shimizu, Shiho; Oohata, Nobutaka; Hino, Motohiro; Yamada, Masato; Isogai, Yasuhiro; Hashimoto, Seiji

doi:10.1016/j.jbiosc.2011.06.002

Cited by 11 publications

(5 citation statements)

References 19 publications

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“…In this study, we found that L. gasseri JM1 possessed the ability to inhibit the adhesion of pathogenic bacteria, which was in agreement with the previous report by Bogovic Matijasić et al (2006) that L. gasseri K7 has significantly inhibited the adhesion of E. coli O8: K88 to Caco-2 cells. In general, lactic acid bacteria protect the host against pathogenic bacteria infection by steric hindrance on the receptors, as well as production of organic acids and bacteriocins (Neeser et al, 2000;Ueda et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus gasseri JM1 with potential probiotic characteristics alleviates inflammatory response by activating the PI3K/Akt signaling pathway in vitro

Sun

Tian

Guo

et al. 2020

Journal of Dairy Science

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Lactobacillus gasseri JM1, a novel strain isolated from infant feces, exhibited common probiotic properties such as high acid tolerance, bile salt tolerance, and adhesion to epithelial Caco-2 cells, suggesting its ability to survive in the gastrointestinal tract and confer potential probiotic action on the host. In the current study, we aimed to evaluate the immunomodulatory activity of L. gasseri JM1 and explore the underlying signaling pathways in vitro. The results showed that pretreatment with L. gasseri JM1 alleviated lipopolysaccharide-induced inflammatory response, as evidenced by downregulation of genes encoding proinflammatory cytokines [IL1B, IL6, IL8, and tumor necrosis factor-α (TNFA)] and upregulation of genes encoding anti-inflammatory cytokines [IL4, IL10, transforming growth factor-β3 (TGFB3), and IFNG]. A high level of gene expression was noted for toll-like receptor 2 and NOD-like receptor 2. Meanwhile, transcriptomic sequencing obtained 84 differentially expressed genes. Kyoto Encyclopedia of Genes and Genomes analysis revealed the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway was activated by L. gasseri JM1 in Caco-2 cells. Inhibitor of PI3K/ Akt played various roles in the release of cytokines, indicating that the pathway was involved in protecting the host against lipopolysaccharide stress. Moreover, whole-genome sequencing revealed fundamental genetic properties of L. gasseri JM1 and provided clues for probiotic characteristics. In summary, the strain could exert immunomodulatory effects via the toll-like receptor 2 and NOD2-mediated PI3K/Akt signaling pathway and be regarded as a potential probiotic.

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Section: Discussionmentioning

confidence: 99%

Lactobacillus gasseri JM1 with potential probiotic characteristics alleviates inflammatory response by activating the PI3K/Akt signaling pathway in vitro

Sun

Tian

Guo

et al. 2020

Journal of Dairy Science

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“…In the next stage, we investigated purification methods that did not use ODS column chromatography. Many kinds of synthetic adsorbents that could be used repeatedly after regeneration, such as SEPABEADS, SP207SS, DIAION HP20SS, and DIAION HP20, were tested, with DIAION HP20SS found to be the most efficient resin synthetic adsorbent. In addition, we found that the most efficient method of regenerating the DIAION HP20SS was: wash with (1) acetone, (2) water, (3) 1:1 acetone:1 M aqueous NaOH, (4) water, (5) 0.1 M aqueous H 2 SO 4 , (6) water, (7) acetone, (8) 0.1 M aqueous NaOH, (9) water, and finally (10) 0.5 M aqueous NaHCO 3 .…”

Section: Results and Discussionmentioning

confidence: 99%

A Challenging Synthesis of the Highly Functionalized Echinocandin ASP9726: A Successor of Micafungin

Yoshida

Hayashida

Morinaga

et al. 2014

Org. Process Res. Dev.

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Here, we describe a practical, scalable, and challenging synthesis of the highly functionalized novel echinocandin ASP9726 (1) starting from the natural product FR901379 (3), which is a starting material of micafungin (2). The synthesis includes transformations that address significant synthetic challenges due to the need to control the chemoselectivity of the reactions during modification of the highly functionalized peptide core. In the present study, we discovered an efficient, highyielding route to ASP9726 (1) that is suitable for large-scale production. Namely, dehydration of carboxamide ( 14) to nitrile (15) was accomplished by use of EDC•HCl with pyridine. Further, the transformation of nitrile (15) to primary amine (17) was conducted via hydrogenation with Sponge Nickel catalyst without decomposition, followed by one-pot debenzylation with Pd/C. Reductive amination between primary amine (17) with dihydroxyacetone (DHA) was accomplished using 2-picoline/borane complex as a reducing agent in MeOH, yielding 66.6 kg of peptide core unit (18). After the C 15 H 31 chain cleavage by bioconversion, reductive amination between the core peptide unit (4) and side chain (10) was achieved in high yield by making use of tert-butyl amine/borane complex as a reducing agent. Consequently, highly pure ASP9726 (1) was obtained in a practical manner without using silica gel or ODS column chromatography purification in any step. Overall yield was drastically increased from 0.71% to 13.8% compared to that of the prior synthetic method.

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“…Through consecutive mutagenesis steps and selection in small‐scale assays, enhanced acylase activity was achieved. When combined with an optimized fermentation setup the hyper‐production of the targeted enzyme reached 65‐fold increase .…”

Section: Strain Developmentmentioning

confidence: 99%

Fermentation Titer Optimization and Impact on Energy and Water Consumption during Downstream Processing

et al. 2018

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A common focus of fermentation process optimization is the product titer. Different strategies to boost fermentation titer target whole‐cell biocatalyst selection, process control, and medium composition. Working at higher product concentrations reduces the water that needs to be removed in the case of aqueous systems and, therefore, lowers the cost of downstream separation and purification. Different approaches to achieve higher titer in fermentation are examined. Energy and water consumption data collected from different Cargill fermentation plants, i.e., ethanol, lactic acid, and 2‐keto‐L‐gulonic acid, confirm that improvements in fermentation titer play a decisive role in downstream economics and environmental footprint.

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Strain selection and scale-up fermentation for FR901379 acylase production by Streptomyces sp. no. 6907

Cited by 11 publications

References 19 publications

Lactobacillus gasseri JM1 with potential probiotic characteristics alleviates inflammatory response by activating the PI3K/Akt signaling pathway in vitro

Lactobacillus gasseri JM1 with potential probiotic characteristics alleviates inflammatory response by activating the PI3K/Akt signaling pathway in vitro

A Challenging Synthesis of the Highly Functionalized Echinocandin ASP9726: A Successor of Micafungin

Fermentation Titer Optimization and Impact on Energy and Water Consumption during Downstream Processing

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