2023
DOI: 10.1101/2023.08.31.555694
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Strain-specific differences in the interactions of the cucumber mosaic virus 2b protein with the viral 1a and host Argonaute 1 proteins

Sam Crawshaw,
Lewis G. Watt,
Alex M. Murphy
et al.

Abstract: The cucumber mosaic virus (CMV) 2b protein is a potent counter-defense protein and symptom determinant that inhibits antiviral silencing by titration of short double-stranded RNAs. Expression of the 2b protein from the CMV Subgroup IA strain Fny-CMV in transgenic Arabidopsis thaliana plants disrupts microRNA-mediated cleavage of host mRNAs by binding ARGONAUTE 1 (AGO1), leading to symptom-like phenotypes. This also triggers AGO2-mediated resistance against CMV and strong resistance to CMVs aphid vectors, which… Show more

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Cited by 2 publications
(5 citation statements)
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“…However, recent work suggests that this is not due to an inability of Subgroup II CMV 2b protein orthologues to form 2b-AGO1 complexes; rather, it is due to differences in intracellular localization. Specifically, AGO1-2b complexes for Subgroup II orthologues (such as the 2b protein encoded by LS-CMV) accumulate almost exclusively in nuclei, but for Subgroup IA and IB 2b orthologues, these complexes also occur in the cytoplasm, consistent with the localization of the pool of AGO1 molecules mediating miRNA-mediated mRNA cleavage [15].…”
Section: Introductionmentioning
confidence: 67%
See 2 more Smart Citations
“…However, recent work suggests that this is not due to an inability of Subgroup II CMV 2b protein orthologues to form 2b-AGO1 complexes; rather, it is due to differences in intracellular localization. Specifically, AGO1-2b complexes for Subgroup II orthologues (such as the 2b protein encoded by LS-CMV) accumulate almost exclusively in nuclei, but for Subgroup IA and IB 2b orthologues, these complexes also occur in the cytoplasm, consistent with the localization of the pool of AGO1 molecules mediating miRNA-mediated mRNA cleavage [15].…”
Section: Introductionmentioning
confidence: 67%
“…Replacement of residues 56-60 with alanine (mutant 2b 56aaa60 ) also abolished co-localization with the CMV 1a protein, while replacement of residues 56-60 of the Fny-CMV 2b with the corresponding sequence from the LS-CMV 2b protein did not completely abolish co-localization with the CMV 1a protein. This was puzzling since the LS-CMV 2b protein is unable to interact with the Fny-CMV 1a protein [15].…”
Section: Residues 56-60 Of the Fny-cmv 2b Protein Are Required For In...mentioning
confidence: 99%
See 1 more Smart Citation
“…Replacement of residues 56-60 with alanine (mutant 2b 56aaa60 ) also abolished co-localization with the CMV 1a protein, whilst replacement of residues 56-60 of the Fny-CMV 2b with the corresponding sequence from the LS-CMV 2b protein did not completely abolish co-localization with the CMV 1a protein. This was puzzling since LS-CMV 2b protein is unable to interact with the Fny-CMV 1a protein (Crawshaw et al, 2023).…”
Section: Resultsmentioning
confidence: 99%
“…However, recent work suggests that this is not due to an inability of Subgroup II CMV 2b protein orthologues to form 2b-AGO1 complexes; rather, it is due to differences in intracellular localization. Specifically, AGO1-2b complexes for Subgroup II orthologues (such as the 2b protein encoded by LS-CMV) accumulate almost exclusively in nuclei, but for Subgroup IA and IB 2b orthologues these complexes also occur in the cytoplasm, consistent with the localization of the pool of AGO1 molecules mediating miRNA-mediated mRNA cleavage (Crawshaw et al, 2023).…”
Section: Introductionmentioning
confidence: 86%