Strain-Specific Effects of Probiotics on Gut Barrier Integrity following Hemorrhagic Shock

Luyer, Misha; Buurman, Wim A.; Hadfoune, M’hamed; Speelmans, Gea; Knol, Jan; Jacobs, Jan; Dejong, Cornelis H. C.; Vriesema, Aldwin; Greve, Jan

doi:10.1128/iai.73.6.3686-3692.2005

Cited by 101 publications

(68 citation statements)

References 36 publications

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“…Barrier impairment holds also true of mucosal surfaces [3]. Previous studies suggest that probiotic therapy would be beneficial in the preservation of gut barrier integrity [17]. Therefore, we can assume that effects seen in our patients could be based on the improvement of barrier function both of the skin and gut.…”

Section: Discussionmentioning

confidence: 97%

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Successful management of mild atopic dermatitis in adults with probiotics and emollients

et al. 2008

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AbstractAtopic dermatitis is characterized by impaired skin and mucous membrane barrier function. Measures improving barrier integrity decrease the influence of environmental factors that might exacerbate inflammation. Ten adult patients with mild-to-moderate atopic dermatitis consumed for three months fermented with potent antioxidative probiotic, L. fermentum ME-3 (DSM 14241) goat milk 200 mg/day. A control group consisted of six patients, not supplemented by probiotic. All patients used emollients regularly. Skin iron levels, glutathione redox ratios (GSSG/GSH), diene conjugate (DC) amounts, blood glutathione status, oxidized low-density lipoprotein (oxLDL), and total antioxidativity was measured at the baseline and after three months. A significant decrease in skin iron levels, DC amounts, and glutathione redox ratio occurred in the probiotic-supplemented group compared to the control group (P < 0.05 for all indices). In the same group, blood levels of oxLDL decreased (p < 0.05), and GSH levels increased (P < 0.001) with concomitant improvement in the GSSG/GSH ratio. Blood antioxidativity markers also showed an improvement. The results of our study demonstrate that regular use of probiotics with antioxidative properties coupled with the use of lipid-containing emollients considerably decreases inflammation and concomitant oxidative stress in adult patients with mild-to-moderate atopic dermatitis. This effect was observed both in the skin and in the blood.

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Section: Discussionmentioning

confidence: 97%

“…Resent evidence also suggests that probiotic strains might preserve gut integrity in several pathological conditions and that the mechanisms underlying this effect might be systemic, i.e. more than manipulation of intestinal microflora alone [17,20].…”

Section: Discussionmentioning

confidence: 99%

Successful management of mild atopic dermatitis in adults with probiotics and emollients

et al. 2008

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“…In the present study, we examined the effects of two intestinal Lactobacillus strains because recent reports suggest that several species of Lactobacillus are probiotics that have protective immunomodulating properties (1,14,27), are able to prevent adhesion of pathogenic bacteria to the intestinal wall (44) and can preserve mucosal integritiy (17). In initial experiments, we determined IL-6 production in cultured Caco-2 cells treated with IL-1␤, live L. paracasei or live L. plantarum, either alone or in combinations.…”

Section: Resultsmentioning

confidence: 99%

Probiotics potentiate IL-6 production in IL-1β-treated Caco-2 cells through a heat shock-dependent mechanism

Reilly¹,

Poylin²,

Menconi³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

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IL-6 may exert anti-inflammatory and protective effects in intestinal mucosa and enterocytes. The influence of probiotics on mucosal and enterocyte IL-6 production is not known. We tested the hypothesis that the probiotic bacteria Lactobacillus paracasei and Lactobacillus plantarum regulate IL-6 production in intestinal epithelial cells. Cultured Caco-2 cells were treated with 1 ng/ml of IL-1β in the absence or presence of different concentrations of L. paracasei or L. plantarum followed by measurement of IL-6 production. The role of heat shock response was examined by determining the expression of heat shock protein 70 (hsp70) and hsp27, by downregulating their expression with small interfering RNA (siRNA), or by treating cells with quercetin. Treatment of the Caco-2 cells with IL-1β resulted in increased IL-6 production, confirming previous reports from this laboratory. Probiotics alone did not influence IL-6 production, but the addition of probitoics to IL-1β-treated cells resulted in a substantial augmentation of IL-6 production. Treatment of the Caco-2 cells with live L. paracasei increased cellular levels of hsp70 and hsp27 and the potentiating effect on IL-6 production was inhibited by quercetin and by hsp70 or hsp27 siRNA. Results suggest that probiotics may enhance IL-6 production in enterocytes subjected to an inflammatory stimulus and that this effect may, at least in part, be heat shock dependent.

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“…The mucosal barrier is often disrupted in IBD patients leading to increased intestinal permeability; therefore a more robust barrier would significantly lessen bacterial translocation and prevent antigenic overload, thereby reducing inflammation. While other probiotics have been shown to enhance barrier function (Madsen et al, 2001;Luyer et al, 2005;Zeng et al, 2008;Pagnini et al, 2010;Zakostelska et al, 2011;Carlsson et al, 2013), they are unlikely to be able to survive and compete within the iron-rich environment of the IBD intestine. Since S. thermophilus NCIMB 41856 can use iron as a growth factor it is able to compete with the resident microflora in order to survive and exert its beneficial effect (Bailey et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Streptococcus thermophilus NCIMB 41856 ameliorates signs of colitis in an animal model of inflammatory bowel disease

Bailey

Vince²,

Williams³

et al. 2017

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Treatment of inflammatory bowel disease (IBD) is mainly based on suppression of symptoms, often with numerous side effects. Trials of probiotics in IBD have frequently produced disappointing results. The majority of probiotics are unusual, since they do not require iron for growth, unlike many bacteria resident in the intestine. The IBD intestine is iron-rich due to bleeding and use of oral iron supplements; conventional probiotics would be rapidly outcompeted. We have evaluated an iron-responsive Streptococcus thermophilus strain for its potential to reduce signs of colitis. Efficacy of S. thermophilus was evaluated in the dextran sodium sulphate mouse model of colitis. Treated animals were given 1×108 cfu S. thermophilus per day and clinical observations were taken daily. At termination, gross and histopathological signs of disease, cellular infiltration, location of bacteria, and cytokine expression in the intestine were determined. S. thermophilus delayed onset of colitis and reduced clinical signs of disease, including bodyweight loss and gastrointestinal bleeding. It reduced bacterial translocation into the colonic tissue. Increased numbers of CD8+ intraepithelial lymphocytes were seen in control animals treated with S. thermophilus. S. thermophilus had no effect on gross pathology, histopathology or cytokine production in either colitic or control animals. We propose that S. thermophilus promotes maintenance of mucosal barrier function which reduces bacterial translocation, thereby reducing immune stimulation and associated inflammation. This allows mucosal healing, reducing gastrointestinal bleeding and weight loss. This could be studied as a locally-acting adjunct or alternative to current IBD treatments.

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Strain-Specific Effects of Probiotics on Gut Barrier Integrity following Hemorrhagic Shock

Cited by 101 publications

References 36 publications

Successful management of mild atopic dermatitis in adults with probiotics and emollients

Successful management of mild atopic dermatitis in adults with probiotics and emollients

Probiotics potentiate IL-6 production in IL-1β-treated Caco-2 cells through a heat shock-dependent mechanism

Streptococcus thermophilus NCIMB 41856 ameliorates signs of colitis in an animal model of inflammatory bowel disease

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