Strain typing of the Mycobacterium avium complex

Correlation between variable-number tandem-repeat-based genotypes and drug susceptibility in Mycobacterium avium isolates

“…Notably, the two-tailed Fisher's exact test indicated a significant difference between MICs of gatifloxacin for NB-Mav and CA-Mav isolates (P=0.032). (2) No such difference in susceptibility profiles between NB-and CAMav was noted for the other antimycobacterial drugs. These findings suggest that the susceptibility of NB-Mav and CAMav to the drugs tested including macrolides, rifamycins, ethambutol, aminoglycosides, isoniazid and quinolones, did not differ significantly.…”

“…To effectively treat MAC diseases, it is important to investigate recent profiles of change in the drug resistance, particularly in relation to the genetic polymorphisms of MAC isolates from patients with different clinical symptoms, pathologic features, medical histories, etc. A number of reports have described the genotyping of MAC using various methods, including insertion sequence (IS) 1245 and IS1311 restriction fragment length polymorphism (RFLP)-, pulsed field gel electrophoresis (PFGE)-, and variable-number tandemrepeat (VNTR)-based typing [2,3]. It has recently been demonstrated that VNTR typing is useful to distinguish and classify MAC isolates from clinical specimens and environmental sources [4,5].…”

Molecular epidemiology of tuberculosis and other mycobacterial infections: main methodologies and achievements

“…Other methods for typing M. avium ssp. avium isolates are the rRNA typing method [278], multilocus enzyme electrophoresis [279, 280], restriction enzyme analysis, field‐inversion gel electrophoresis, RFLP analysis with random DNA probes, typing on the basis of chemical analysis of cell wall components and by the presence of plasmids [281]. PCR‐based methods of typing M. avium are randomly amplified polymorphic DNA (RAPD) analysis [282] and PCR amplification of the chromosomal DNA between IS 1245 and IS 1311.…”