Strains of Moroccan watermelon mosaic virus Isolated from Lagenaria breviflorus and Coccinia barteri in Calabar, Southeastern Nigeria

Owolabi, A. T.; Rabenstein, Frank; Ehrig, Fred; Edgar, Marcos; Vetten, H. J.

doi:10.3923/ijv.2012.258.270

Cited by 20 publications

(12 citation statements)

References 23 publications

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“…In African countries, the virus has been reported in South Africa (Van Der Meer and Garnett, 1987), Sudan (Lecoq et al, 2001), Democratic Republic of Congo (Arocha et al, 2008), Tunisia (Yakoubi et al, 2008) and Nigeria (Owolabi et al, 2012). The virus has also been reported in Spain (Quiot-Douine et al, 1990) and Italy (Roggero et al, 1998).…”

Section: Introductionmentioning

confidence: 89%

“…Characterization and identification of a potyvirus causing mosaic disease of Cucurbita moschata (Owolabi et al, 2011), strains of Moroccan watermelon mosaic virus isolated from Lagenaria breviflorus and Coccinia barteri (Owolabi et al, 2012).…”

Section: Intmentioning

confidence: 99%

“…A survey of literature on virus diseases of cucurbits revealed that more than 39 well characterized viruses are distributed in unrelated genera, such as Begomo virus, Crinivirus, Cucumovirus, Polerovirus, Tospovirus, Ipomovirus, Tobamovirus and Potyvirus, which are known to naturally infect cucurbits (Antignus et al, 2001;Salem et al, 2007;Kneirim et al, 2010;Gholamalizadeh et al, 2008;Brown et al, 2002;Owolabi et al, 2008Owolabi et al, , 2011Owolabi et al, , 2012.…”

Section: Introductionmentioning

confidence: 99%

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Physiological and Biochemical Changes in Cucurbita moschata Duch. Ex. Poir Inoculated with a Nigerian Strain of Moroccan Watermelon Mosaic Virus (MWMV): Lagenaria breviflora Isolate

Mofunanya¹,

Edu²

2015

International J. of Plant Pathology

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This study was carried out to determine physiological and biochemical changes in C. moschata inoculated with a Nigerian strain of MWMV isolated from Lagenaria breviflora in Calabar, Nigeria. The virus infection resulted in significant (p#0.05) reductions in plant height, leaf area and number of leaves produces with highest percentage difference of 39.3% (42 PID), 60.9% (35 PID) and 25.7% (49 PID), respectively. There was a significant (p#0.05) difference in chlorophyll content between inoculated and healthy plants. The chlorophyll content of inoculated plants at all stages of growth was lower compared with the healthy plants. Chlorophyll b content was higher than Chl a in both inoculated and healthy plants. Chlorophyll a, b and total Chlorophyll were significantly reduced in inoculated plants when compared with the healthy ones. Of the twelve photochemicals screened, alkaloids, glycosides, saponins, tannins, terpenoids, steroids, polyphenols and reducing sugars were present while phlobatanins, anthraquinones and hydroxymethyl anthraquinones were absent. Moroccan watermelon mosaic virus engendered reductions in alkaloids (32.1%), reducing sugars (42.0%), saponins (33.6%), terpenoids (25.0%) and steroids (15.0%) and increase in inoculated samples of glycosides (14.8%) and flavonoids (12.5%). Effect of MWMV on proximate compositions of C. moschata revealed significant p#0.05) reductions in lipid (25.0%) and ash (14.6%) and significant (p$0.05) increases in protein (23.4%) and fibre (10.5%). Effect of the virus on carotene, vitamin A and C were insignificant. Infection of MWMV also led to significant decreases in the contents of K (48%), Fe (28.6%), Zn (25.0%) and Mg (22.0%), while Cu recorded 100% increase in inoculated samples. Marginal increase in Ca and P were recorded in inoculated samples. Infection of MWMV on antioxidants caused significant increase in inoculated samples of hydrocyanic acid and insignificant increases in total and soluble oxalate and phytic acid. Increases recorded in the antioxidant contents in plants inoculated with MWMV resulted in reduced bioavailability of minerals in the vegetable. Reduction in chlorophyll contents in inoculated plants would be a major factor explaining poor growth of host plants with attendant reductions in mineral elements of C. moschata. Changes in physiological and biochemical parameters induced by MWMV altered vital metabolic processes of C. moschata.

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Section: Introductionmentioning

confidence: 89%

Section: Intmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Physiological and Biochemical Changes in Cucurbita moschata Duch. Ex. Poir Inoculated with a Nigerian Strain of Moroccan Watermelon Mosaic Virus (MWMV): Lagenaria breviflora Isolate

Mofunanya¹,

Edu²

2015

International J. of Plant Pathology

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“…Moreover, MWMV, AWMV, ZSSV, SuWMV and WMVBV were identified in Africa, suggesting that the PRSV cluster underwent an important diversification in Africa [25]. Out of these viruses present in Africa, MWMV is the widespread one having been reported in all African regions [3,[26][27][28][29][30][31][32]. The HTS in this study made the detection of ZSSV on infected leaf sample possible.…”

Section: Discussionmentioning

confidence: 73%

High-throughput sequencing of virus-infected Cucurbita pepo samples revealed the presence of Zucchini shoestring virus in Zimbabwe

2020

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Objectives: Plant-infecting viruses remain a serious challenge towards achieving food security worldwide. Cucurbit virus surveys were conducted in Zimbabwe during the 2014 and 2015 growing seasons. Leaf samples displaying virus-like symptoms were collected and stored until analysis. Three baby marrow samples were subjected to nextgeneration sequencing and the data generated were analysed using genomics technologies. Zucchini shoestring virus (ZSSV), a cucurbit-infecting potyvirus previously described in South Africa was one of the viruses identified. The genomes of the three ZSSV isolates are described analysed in this note. Results:The three ZSSV isolates had the same genome size of 10,297 bp excluding the polyA tail with a 43% GC content. The large open reading frame was found at positions 69 to 10,106 on the genome and encodes a 3345 amino acids long polyprotein which had the same cleavage site sequences as those described on the South African isolate except for the P1-pro site. Genome sequence comparisons of all the ZSSV isolates showed that the isolates F7-Art and S6-Prime had identical sequence across the entire genome while sharing 99.06% and 99.34% polyprotein nucleotide and amino acid sequence identities, respectively with the isolate S7-Prime.

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“…A strain of the Moroccan watermelon mosaic virus, a Potyvirus, can infest Coccinia barteri (Owolabi et al 2012), whereas the infection of Coccinia sessilifolia with this virus, maybe a different strain, failed (van der Meer and Garnett 1987). …”

Section: Plant – Biotic Environment Interactionsmentioning

confidence: 99%

Monograph of Coccinia (Cucurbitaceae)

Holstein¹

2015

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This monograph deals with all 95 names described in the Cucurbitaceae genus Coccinia and recognizes 25 species. Taxonomic novelties are Coccinia adoensis var. aurantiaca (C.Jeffrey) Holstein, stat. nov., Coccinia sessilifolia var. variifolia (A.Meeuse) Holstein, stat. nov., and Coccinia adoensis var. jeffreyana Holstein, var. nov. For the 25 species 3157 collections were examined, of which 2024 were georeferenced to produce distribution maps. All species are distributed in sub-Saharan Africa with one species, Coccinia grandis, extending from Senegal in West Africa east to Indonesia and being naturalized on Pacific Islands, in Australia, the Caribbean, and South America. Coccinia species are dioecious creepers or climbers with simple or bifid tendrils that occupy a range of habitats from arid scrubland, woodlands to lowland rainforest and mist forest. The corolla of Coccinia species is sympetalous, usually pale yellow to orange, and 1 to 4.5 cm long. Pollination is by bees foraging for pollen or nectar. After pollination, the developing ovary often exhibits longitudinal mottling, which usually disappears during maturation. All species produce berries with a pericarp in reddish colors (orange-red through to scarlet red), hence the generic name. The globose to cylindrical fruits contain numerous grayish-beige flat to lenticular seeds. Chromosome numbers are 2n = 20, 24, and 22 + XX/XY. Many Coccinia species are used for food, either as roasted tubers, greens as spinach, or the fruits as vegetables. Medicinal value is established in Coccinia grandis, of which leaves and sap are used against diabetes.

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Strains of Moroccan watermelon mosaic virus Isolated from Lagenaria breviflorus and Coccinia barteri in Calabar, Southeastern Nigeria

Cited by 20 publications

References 23 publications

Physiological and Biochemical Changes in Cucurbita moschata Duch. Ex. Poir Inoculated with a Nigerian Strain of Moroccan Watermelon Mosaic Virus (MWMV): Lagenaria breviflora Isolate

Physiological and Biochemical Changes in Cucurbita moschata Duch. Ex. Poir Inoculated with a Nigerian Strain of Moroccan Watermelon Mosaic Virus (MWMV): Lagenaria breviflora Isolate

High-throughput sequencing of virus-infected Cucurbita pepo samples revealed the presence of Zucchini shoestring virus in Zimbabwe

Monograph of Coccinia (Cucurbitaceae)

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