Strategic intensification in butanol production by exogenous amino acid supplementation: Fermentation kinetics and thermodynamic studies

Abstract

Highlights Exogenous addition of tryptophan:phenylalanine triggered butanol production  Amino acid addition after 8 h in batch cultivation resulted in 12.43±0.1g/L butanol  Fed-batch with in-situ product recovery was promising for improved butanol titer  ABE production follow first order kinetics in respect to intermediate concentration  Thermodynamics of ABE fermentation was studied to evaluate reaction feasibility Abstract Amino acids are vital precursors in many biochemical production pathways in addition to efficient nitrogen source which could enhance microbial growth yields. Therefore, in present study, the effect of amino acids from aliphatic and aromatic family was comprehensively evaluated in batch and integrated fed batch fermentation system. Clostridium acetobutylicum NRRL B-527 was able to utilize 54.15 ± 1.0 g/L glucose to produce 12.43 ± 0.10 g/L butanol under batch cultivation. Interestingly, a significant step up in butanol titer (20.82 ± 0.33 g/L) was achieved by using fedbatch fermentation process integrated with liquid-liquid extraction module. Besides, mathematical modeling studies demonstrated the best fitting of experimental data with first order reaction kinetics. Overall, an enhancement in solvent titer by induction of essential cellular components coupled with advance bioprocess strategy was successfully utilized in this study for its further applications.

Efficient Strategy to Alleviate the Inhibitory Effect of Lignin-Derived Compounds for Enhanced Butanol Production

“…Our recent study described that tryptophan drives more metabolic flux toward butanol, while arginine could be useful in energy (ATP) build up. 38 Moreover, it has been demonstrated that pelleting soybean meal with calcium lignosulfonate protects protein degradation in the rumen. 39 Similarly, lignosulfonates might be coupled with added soy meal and result in a lowered inhibitory effect in the present investigation.…”

“…Besides, tryptophan and arginine also have a positive role in ABE fermentation. Our recent study described that tryptophan drives more metabolic flux toward butanol, while arginine could be useful in energy (ATP) build up . Moreover, it has been demonstrated that pelleting soybean meal with calcium lignosulfonate protects protein degradation in the rumen .…”

Enhancing bacterial cellulose production of Komagataeibacter nataicola through fermented coconut water by Saccharomyces cerevisiae: A metabonomics approach

“…Amino acids and their derivatives serve as crucial precursors in various metabolic pathways and play significant roles in microbial metabolism and function ( Nimbalkar et al, 2019 ). In a study conducted by Gomes et al (2020) , it was found that aspartic acid, phenylalanine, and serine enhanced BC production in a static culture environment.…”

Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia

“…According to the metabolic pathway and physiological characteristics, various organic acids (such as acetate, butyrate, amino acids, and lactic acid) could serve as alternative substrates for butanol production, and then maintain the robust expression of enzymes associated during the solventogenic and solventogenesis phase [169,170]. Interestingly, when butyrate was the sole carbon source, only 0.2 g/L of butanol was produced, but the production of butanol significantly increased to 10 g/L when both butyric acid and glucose were present, suggesting that butyric acid may be an important factor triggering solvent production [171].…”