“…Moreover, aspects like bioavailability of the toxic compounds, their synergistic and antagonistic effects, as well as the metabolism in the organisms are not taken into account. Thus, in order to acknowledge the complex situation in the field a holistic assessment in environmental monitoring is demanded, that considers not only the concentrations of selected compounds, but integrates them with the toxic effects the environmental sample may induce (Brils, 2008;Heugens et al, 2001;Hollert et al, 2007Hollert et al, , 2009Malaj et al, 2014;SedNet, 2004;Wernersson et al, 2015). One approved holistic assessment method is the triad approach by Chapman (1990), with in situ investigations of biomarkers in fish suggested as additional line of evidence (Chapman and Hollert, 2006).…”