Strategies for advanced personalized tuberculosis diagnosis: Current technologies and clinical approaches

Chen, Xuerong; Hu, Tony

doi:10.1093/pcmedi/pbaa041

Cited by 11 publications

(8 citation statements)

References 49 publications

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“…On our standard curve, this equates to 0.1 μM (160.3 ngmL –1 ). This proof-of-concept demonstration is for CFP-10 suspended in a homogeneous PBS solution, and performance will certainly change with samples spiked in commercialized human serum and clinical samples . However, with future improvements in sample filtration, , optimization of antibody–antigen pairing, and recycling steps, this sensor is expected to show similar functionality for more complex human samples.…”

Section: Resultsmentioning

confidence: 99%

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Silicon Nanodisk Huygens Metasurfaces for Portable and Low-Cost Refractive Index and Biomarker Sensing

Oguntoye

Simone

Padmanabha

et al. 2022

ACS Appl. Nano Mater.

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Biomarker detection and bulk refractive index sensing are important across multiple industries ranging from early medical diagnosis to chemical process quality control. The bulky size, high cost, and complex architecture of existing refractive index and biomarker sensing technologies limit their use to highly skilled environments like hospitals, large food processing plants, and research labs. Here, we demonstrate a compact and inexpensive refractive index sensor based on resonant dielectric photonic nanoantenna arrays or metasurfaces. These dielectric resonances support Mie dipole and asymmetric resonances that shift with changes in their external environment. A single-wavelength transmission measurement in a portable (<250 in. 3 ), low-cost (<$4000) sensor shows sensitivity to 1.9 × 10 –6 change in the fluid refractive index without the use of a spectrometer or other complex optics. Our sensor assembly allows for measurements using multiple metasurfaces with identical resonances or varying resonance types for enhanced diagnostics on the same chip. Furthermore, a 10 kDa culture filtrate peptide CFP-10, a marker for human tuberculosis, is detected with our sensor with 10 pM resolution. This system has the potential to enable facile, fast, and highly sensitive measurements with adequate limits of detection for personalized biomedical diagnoses.

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Section: Resultsmentioning

confidence: 99%

“…Biomarker Detection Results. We use the bioassay described in the Experimental Section to measure a wide range of concentrations of the TB antigen CFP-10 (one of the top two biomarkers for detecting TB) 47 in a phosphate buffer solution (PBS). The metasurface-based sensor produces the results seen in Figure 7f,g.…”

Section: Experimental Methodologymentioning

confidence: 99%

Silicon Nanodisk Huygens Metasurfaces for Portable and Low-Cost Refractive Index and Biomarker Sensing

Oguntoye

Simone

Padmanabha

et al. 2022

ACS Appl. Nano Mater.

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“…In conclusion, the research field of ncRNAs in M. tuberculosis infection has met a certain degree of success in pathogenesis and biomarker exploration and holds broad promise. However, several challenges still need to be addressed before these molecules can be seamlessly integrated into clinical practice to enrich personalized and creative diagnostic strategies ( 211 , 212 ), and further direct treatment decision-making in TB, especially for the management of drug-resistant patients.…”

Section: Conclusion Remarksmentioning

confidence: 99%

Immune regulation and emerging roles of noncoding RNAs in Mycobacterium tuberculosis infection

Liang

Ma²,

Gong³

et al. 2022

Front. Immunol.

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Tuberculosis, caused by Mycobacterium tuberculosis, engenders an onerous burden on public hygiene. Congenital and adaptive immunity in the human body act as robust defenses against the pathogens. However, in coevolution with humans, this microbe has gained multiple lines of mechanisms to circumvent the immune response to sustain its intracellular persistence and long-term survival inside a host. Moreover, emerging evidence has revealed that this stealthy bacterium can alter the expression of demic noncoding RNAs (ncRNAs), leading to dysregulated biological processes subsequently, which may be the rationale behind the pathogenesis of tuberculosis. Meanwhile, the differential accumulation in clinical samples endows them with the capacity to be indicators in the time of tuberculosis suffering. In this article, we reviewed the nearest insights into the impact of ncRNAs during Mycobacterium tuberculosis infection as realized via immune response modulation and their potential as biomarkers for the diagnosis, drug resistance identification, treatment evaluation, and adverse drug reaction prediction of tuberculosis, aiming to inspire novel and precise therapy development to combat this pathogen in the future.

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“…2 Various diagnostic methods, including sputum smear microscopy, Xpert MTB/RIF, and Hain line test, have facilitated the rapid identification of the Mycobacterium complex (MTBC) responsible for TB. 3 However, the confirmation of drug-resistant TB diagnosis solely on the basis of these factors remains uncertain.…”

mentioning

confidence: 99%

“…TB patients can be categorized into three groups: latent TB infection (LTBI), active TB, and subclinical TB, each with distinct characteristics and treatment options . Various diagnostic methods, including sputum smear microscopy, Xpert MTB/RIF, and Hain line test, have facilitated the rapid identification of the Mycobacterium complex (MTBC) responsible for TB . However, the confirmation of drug-resistant TB diagnosis solely on the basis of these factors remains uncertain.…”

mentioning

confidence: 99%

Revolutionizing Tuberculosis Treatment: Uncovering New Drugs and Breakthrough Inhibitors to Combat Drug-Resistant Mycobacterium tuberculosis

Verma,

Naik,

Kumar

et al. 2023

ACS Infect. Dis.

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Tuberculosis (TB) is a global health threat that causes significant mortality. This review explores chemotherapeutics that target essential processes in Mycobacterium tuberculosis, such as DNA replication, protein synthesis, cell wall formation, energy metabolism, and proteolysis. We emphasize the need for new drugs to treat drug-resistant strains and shorten the treatment duration. Emerging targets and promising inhibitors were identified by examining the intricate biology of TB. This review provides an overview of recent developments in the search for anti-TB drugs with a focus on newly validated targets and inhibitors. We aimed to contribute to efforts to combat TB and improve therapeutic outcomes.

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Strategies for advanced personalized tuberculosis diagnosis: Current technologies and clinical approaches

Cited by 11 publications

References 49 publications

Silicon Nanodisk Huygens Metasurfaces for Portable and Low-Cost Refractive Index and Biomarker Sensing

Silicon Nanodisk Huygens Metasurfaces for Portable and Low-Cost Refractive Index and Biomarker Sensing

Immune regulation and emerging roles of noncoding RNAs in Mycobacterium tuberculosis infection

Revolutionizing Tuberculosis Treatment: Uncovering New Drugs and Breakthrough Inhibitors to Combat Drug-Resistant Mycobacterium tuberculosis

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