Strategies for bacterial tagging and gene expression in plant-host colonization studies

Ramos, Humberto J.O.; Yates, M. G.; Pedrosa, Fábio O.; Souza, Emanuel M.

doi:10.1016/j.soilbio.2011.03.029

Cited by 11 publications

(11 citation statements)

References 136 publications

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“…One of the most widespread techniques used in the localization of indigenous bacterial endophytes, in endophyte competition and colonization studies, and in identifying active bacteria, is green fluorescent protein (GFP) labeling (see reviews by Schmid et al, 2006; Ramos et al, 2011). Insertion of GFP requires conjugation of the target organism with a carrier organism (e.g., E. coli containing a plasmid with GFP and the Tn5 transposon) (Bhatia et al, 2002).…”

Section: Methods To Study the Microbiome Inside The Rootmentioning

confidence: 99%

Inside the root microbiome: Bacterial root endophytes and plant growth promotion

Gaiero

McCall

Thompson

et al. 2013

American J of Botany

559

267

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Bacterial root endophytes reside in a vast number of plant species as part of their root microbiome, with some being shown to positively influence plant growth. Endophyte community structure (species diversity: richness and relative abundances) within the plant is dynamic and is influenced by abiotic and biotic factors such as soil conditions, biogeography, plant species, microbe-microbe interactions and plant-microbe interactions, both at local and larger scales. Plant-growth-promoting bacterial endophytes (PGPBEs) have been identified, but the predictive success at positively influencing plant growth in field conditions has been limited. Concurrent to the development of modern molecular techniques, the goal of predicting an organism's ability to promote plant growth can perhaps be realized by more thorough examination of endophyte community dynamics. This paper reviews the drivers of endophyte community structure relating to plant growth promotion, the mechanisms of plant growth promotion, and the current and future use of molecular techniques to study these communities.

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Section: Methods To Study the Microbiome Inside The Rootmentioning

confidence: 99%

Inside the root microbiome: Bacterial root endophytes and plant growth promotion

Gaiero

McCall

Thompson

et al. 2013

American J of Botany

559

267

View full text Add to dashboard Cite

show abstract

“…BL21-AI(IY,1amb-immE3) was constructed only by the introduction of two plasmids and without genome editing. Although plasmids are useful for easy gene introduction, their use has a problem for environmental applications because of possible horizontal gene transfer to other bacteria ( Ramos et al, 2011 ). In the case of BL21-AI(IY,1amb-immE3), the plasmid carrying colE3-immE3 is not transmittable because the immE3 in this plasmid is a loss-of-function mutant if the recipient strains are not amber-suppressor mutants.…”

Section: Discussionmentioning

confidence: 99%

“…Outside of the controlled areas, the organisms should die because the substance does not exist or is not present in high enough amounts. Contrary to this expectation, however, some papers have reported that some contained bacteria can survive in the natural environment ( Ramos et al, 2011 ). For example, a passively-contained strain of the bacterium Rhyzobium meliloti ( thyA − ) became colonized in the presence of alfalfa which could supply either some thymine or thymidine as root exudates ( O’Flaherty et al, 1995 ).…”

Section: Introductionmentioning

confidence: 92%

“…For microbes, this technique involves genetically programing the organisms to grow only in the laboratory and to die in the natural environment. Biological containment has been investigated for genetically-engineered microbes since the initiation of biotechnology research because of their usefulness for environmental detoxification ( Paul, Pandey & Jain, 2005 ; Ramos et al, 2011 ), biocontrol ( Migheli, 2001 ), and live vaccines ( Daniel et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

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An engineered bacterium auxotrophic for an unnatural amino acid: a novel biological containment system

Kato

2015

PeerJ

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Biological containment is a genetic technique that programs dangerous organisms to grow only in the laboratory and to die in the natural environment. Auxotrophy for a substance not found in the natural environment is an ideal biological containment. Here, we constructed an Escherichia coli strain that cannot survive in the absence of the unnatural amino acid 3-iodo-L-tyrosine. This synthetic auxotrophy was achieved by conditional production of the antidote protein against the highly toxic enzyme colicin E3. An amber stop codon was inserted in the antidote gene. The translation of the antidote mRNA was controlled by a translational switch using amber-specific 3-iodo-L-tyrosine incorporation. The antidote is synthesized only when 3-iodo-L-tyrosine is present in the culture medium. The viability of this strain rapidly decreased with less than a 1 h half-life after removal of 3-iodo-L-tyrosine, suggesting that the decay of the antidote causes the host killing by activated colicin E3 in the absence of this unnatural amino acid. The contained strain grew 1.5 times more slowly than the parent strains. The escaper frequency was estimated to be 1.4 mutations (95% highest posterior density 1.1–1.8) per 105 cell divisions. This containment system can be constructed by only plasmid introduction without genome editing, suggesting that this system may be applicable to other microbes carrying toxin-antidote systems similar to that of colicin E3.

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“…A visão acerca da caracterização dos microrganismos como "contaminantes" vem sendo alterada nos últimos anos, e um aumento no número de trabalhos tem atribuído à presença dos microrganismos, como exsudados de endófitos, que podem beneficiar ou simplesmente, não interferir no crescimento e desenvolvimento das microplantas in vitro (ARDANOV et al, 2011;PROCÓPIO, 2004;RAMOS et al, 2011;. Para Thomas et al (2008b) (ANDREOTE et al, 2009;IZUMI et al, 2008;PAMPHILE;SCHULZ;BOYLE, 2005;NOWAK, 2000) (CALVETE et al, 2002;PREECE;SUTTER, 1991).…”

Section: Análises Estatísticasunclassified

Atuação da manifestação bacteriana no desenvolvimento in vitro de clones de Eucalyptus benthamii Maiden & Cambage

Piotto¹

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Strategies for bacterial tagging and gene expression in plant-host colonization studies

Cited by 11 publications

References 136 publications

Inside the root microbiome: Bacterial root endophytes and plant growth promotion

Inside the root microbiome: Bacterial root endophytes and plant growth promotion

An engineered bacterium auxotrophic for an unnatural amino acid: a novel biological containment system

Atuação da manifestação bacteriana no desenvolvimento in vitro de clones de Eucalyptus benthamii Maiden & Cambage

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