Strategies for cell manipulation and skeletal tissue engineering using high-throughput polymer blend formulation and microarray techniques

Khan, Ferdous; Tare, Rahul S.; Kanczler, Janos M.; Oreffo, Richard O.C.; Bradley, Mark

doi:10.1016/j.biomaterials.2009.11.101

Cited by 73 publications

(68 citation statements)

References 62 publications

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“…While we have shown the ability of plasma treated polymer electrospun scaffolds to support kidney cell life further investigation is needed to determine optimal surface chemistry to enhance cell response; this could be done using high throughput systems such as the microarray technique (Khan et al 2010). …”

Section: Discussionmentioning

confidence: 99%

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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There is a pressing need for further advancement in tissue engineering of functional organs with a view to providing a more clinically relevant model for drug development and reduce the dependence on organ donation. Polymer based scaffolds, such as polycaprolactone (PCL), have been highlighted as a potential avenue for tissue engineered kidneys, but there is little investigation down this stream.Focus within kidney tissue engineering has been on 2-dimensional cell culture and decellularised tissue. Electrospun polymer scaffolds can be created with a variety of fibre diameters and have shown a great potential in many areas. The variation in morphology of tissue engineering scaffold has been shown to effect the way cells behave and integrate. In this study we examined the cellular response to scaffold architecture of novel electrospun scaffold for kidney tissue engineering. Fibre diameters of 1.10±0.16 µm and 4.49±0.47 µm were used with 3 distinct scaffold architectures. Traditional random fibres were spun onto a mandrel rotating at 250 rpm, aligned at 1800 rpm with novel cryogenic fibres spun onto a mandrel loaded with dry ice rotating at 250 rpm. Human kidney epithelial cells were grown for 1 and 2 weeks. Fibre morphology had no effect of cell viability in scaffolds with a large fibre diameter but significant differences were seen in smaller fibres. Fibre diameter had a significant effect in aligned and cryogenic scaffold. Imaging detailed the differences in cell attachment due to scaffold differences. These results show that architecture of the scaffold has a profound effect on kidney cells; whether that is effects of fibre diameter on the cell attachment and viability or the effect of fibre arrangement on the distribution of cells and their alignment with fibres. Results demonstrate that PCL scaffolds have the capability to maintain kidney cells life and should be investigated further as a potential scaffold in kidney tissue engineering.

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Section: Discussionmentioning

confidence: 99%

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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“…[ 6 ] Also, scaffolds produced by blending of poly( L -lactide) and polycaprolactone in different proportions signifi cantly improved human stem cell attachment and growth when compared to homopolymers. [ 7 ] This blended polymer also exhibited characteristics favorable for bone regeneration in critical defects in ovine tibiae. [ 8 ] It has been shown that the nanoscale architecture of bioengineered scaffold materials generates bioactive scaffolds, which can substitute for the native extracellular matrix and enable spatiotemporal release of relevant growth factors.…”

Section: Introductionmentioning

confidence: 97%

In Vivo Host Response and Degradation of Copolymer Scaffolds Functionalized with Nanodiamonds and Bone Morphogenetic Protein 2

Suliman

Sun

Pedersen

et al. 2016

Adv Healthcare Materials

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The aim is to evaluate the effect of modifying poly[( L -lactide)-co-(ε-caprolactone)] scaffolds (PLCL) with nanodiamonds (nDP) or with nDP+physisorbed BMP-2 (nDP+BMP-2) on in vivo host tissue response and degradation. The scaffolds are implanted subcutaneously in Balb/c mice and retrieved after 1, 8, and 27 weeks. Molecular weight analysis shows that modifi ed scaffolds degrade faster than the unmodifi ed. Gene analysis at week 1 shows highest expression of proinfl ammatory markers around nDP scaffolds; although the presence of infl ammatory cells and foreign body giant cells is more prominent around the PLCL. Tissue regeneration markers are highly expressed in the nDP+BMP-2 scaffolds at week 8. A fi brous capsule is detectable by week 8, thinnest around nDP scaffolds and at week 27 thickest around PLCL scaffolds. mRNA levels of ALP, COL1α2, and ANGPT1 are signifi cantly upregulating in the nDP+BMP-2 scaffolds at week 1 with ectopic bone seen at week 8. Even when almost 90% of the scaffold is degraded at week 27, nDP are observable at implantation areas without adverse effects. In conclusion, modifying PLCL scaffolds with nDP does not aggravate the host response and physisorbed BMP-2 delivery attenuates infl ammation while lowering the dose of BMP-2 to a relatively safe and economical level.

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“…Hydrogels of the chitosan and poly(ethylenimine) binary polymer blend, identifi ed from the microarray platform, were demonstrated to promote proliferation, prevent fi broblastic dedifferentiation and support TGF-β-mediated chondrogenic differentiation of the encapsulated human foetal skeletal cell population [ 30 ]. Furthermore, 3-D scaffolds of the poly( L -lactic acid) and poly(caprolactone) polymer blend, identifi ed from the microarray platform, displayed remarkable trabecular bone-like microarchitecture, supported viability, proliferation and BMP-2-mediated osteogenic differentiation of SSCs in vitro, and facilitated new bone regeneration by SSCs in vivo [ 31 ]. A similar approach was employed to demonstrate the potential of translating HT polymer chemical microarray strategies through in vitro analysis to identify functional ternary polymer blends for in vivo bone repair [ 32 ].…”

Section: Rapid Microscale Screening Of Biomaterial-skeletal Cell Intementioning

confidence: 96%

Microscale Approaches for Molecular Regulation of Skeletal Development

Tare

Gothard

Kanczler

et al. 2016

Microscale Technologies for Cell Engineering

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Strategies for cell manipulation and skeletal tissue engineering using high-throughput polymer blend formulation and microarray techniques

Cited by 73 publications

References 62 publications

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

In Vivo Host Response and Degradation of Copolymer Scaffolds Functionalized with Nanodiamonds and Bone Morphogenetic Protein 2

Microscale Approaches for Molecular Regulation of Skeletal Development

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