Strategies for enhancing microbial tolerance to inhibitors for biofuel production: A review

Wang, Shizeng; Sun, Xinxiao; Yuan, Qipeng

doi:10.1016/j.biortech.2018.03.064

Cited by 137 publications

(60 citation statements)

References 99 publications

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“…However, most of the effective ILs used to solubilize lignocellulosic biomass strongly inhibit microbial growth, reducing the efficiency for the entire process . Importantly, researches on the isolation of ILs‐tolerant microorganisms and their enzymes have started . Xu el al.…”

Section: Ionic Liquid Tolerant Microorganisms For Biocatalysis/biotramentioning

confidence: 99%

An Overview on the Toxicological Properties of Ionic Liquids toward Microorganisms

Sivapragasam

Moniruzzaman

Goto

2020

Biotechnology Journal

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Ionic liquids (ILs), a class of materials with unique physicochemical properties, have been used extensively in the fields of chemical engineering, biotechnology, material sciences, pharmaceutics, and many others. Because ILs are very polar by nature, they can migrate into the environment with the possibility of inclusion in the food chain and bioaccumulation in living organisms. However, the chemical natures of ILs are not quintessentially biocompatible. Therefore, the practical uses of ILs must be preceded by suitable toxicological assessments. Among different methods, the use of microorganisms to evaluate IL toxicity provides many advantages including short generation time, rapid growth, and environmental and industrial relevance. This article reviews the recent research progress on the toxicological properties of ILs toward microorganisms and highlights the computational prediction of various toxicity models.

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Section: Ionic Liquid Tolerant Microorganisms For Biocatalysis/biotramentioning

confidence: 99%

An Overview on the Toxicological Properties of Ionic Liquids toward Microorganisms

Sivapragasam

Moniruzzaman

Goto

2020

Biotechnology Journal

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“…The challenge is thus to address accumulative toxicity by generating multi‐inhibitor resistance phenotypes. This, however, requires in‐depth genetic knowledge of yeast metabolism and stress responses, which is currently still lacking, as strain engineering must avoid the excessive burden or metabolic expense during overexpression that dampen or outweigh the introduced advantage at the cost of productivity . Thus, strain generation relies on the identification of additional inhibitor resistance genes with broader ranges of inhibitor resistance to generate efficient multi‐resistant strains with the minimum burden.…”

Section: Expanding S Cerevisiae Inhibitor Resistancementioning

confidence: 99%

“…Energy and climate concerns have spurred innovative research into the bioconversion of lignocellulose biomass towards carbon‐neutral bioethanol and related bio‐chemicals . A large array of vegetative herbaceous materials – for example, agricultural (straw, sugarcane bagasse) and forestry (birch, spruce) residues, food and municipal waste, and various cellulosic rich industrial waste streams – are available as abundant low‐cost lignocellulose feedstocks . The bioconversion of lignocelluloses towards bioethanol is often based on a fermentation platform with Saccharomyces cerevisiae as biocatalyst, largely because of its long‐standing use in traditional fermentation industries .…”

Section: Introductionmentioning

confidence: 99%

“…furan aldehydes, aliphatic acids, phenolic compounds, inorganic ions, aromatics, and bioalcohols, which greatly decrease the efficiency of the downstream hydrolysis and fermentation steps . Moreover, the chemical composition of the lignocellulose feed, and the severity of the pretreatment process, play a critical role in the formation and final concentrations of microbial inhibitors . The array of available feedstocks coupled with different pretreatment options therefore results in variable combinations and concentrations of microbial inhibitors (Fig.…”

Section: Introductionmentioning

confidence: 99%

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Overcoming lignocellulose‐derived microbial inhibitors: advancing the Saccharomyces cerevisiae resistance toolbox

Brandt

Jansen

Görgens

et al. 2019

Biofuels Bioprod Bioref

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Lignocellulose-derived biofuels present an attractive carbon-neutral alternative to fossil fuels amidst growing global energy and climate concerns. Bioethanol in particular, has been shown to be a viable additive to and / or replacement for petroleum in countries such as Brazil. The bioconversion of lignocellulose biomass to bioethanol is a developing technology with the yeast Saccharomyces cerevisiae playing a pivotal role in the fermentation-based processes. This yeast however, is challenged to ferment under harsh conditions, specifically, during exposure to lignocellulose-derived microbial inhibitors that are formed during pretreatment processes. This detrimentally affects biocatalyst performance, ultimately decreasing ethanol productivity and yield. To this end, S. cerevisiae strains are in development to increase the yeast microbial inhibitor resistance towards cost-effective cellulosic bioethanol production. This review discusses the current status of inhibitor resistance in S. cerevisiae strains and perspectives on the future of multi-tolerant phenotypes with a specific focus on existing and emerging strain development strategies designed to improve resistance phenotypes.

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“…A major hurdle to microbial production of biorenewable fuels and chemicals at economically viable titers, rates and yields is the fact that many of the target products, along with certain types of biomass-derived substrates, are harmful to the production organism [1][2][3][4]. One strategy for addressing this problem is to increase the robustness of the production organism to the problematic inhibitor, such as through evolutionary or targeted strain development, [3,[5][6][7]. In many cases, damage of the microbial cell membrane is a substantial component of the microbial inhibition, and thus the membrane is an attractive engineering target [8][9][10][11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Streamlined assessment of membrane permeability and its application to membrane engineering of Escherichia coli for octanoic acid tolerance

Santoscoy

Jarboe

2019

Journal of Industrial Microbiology and Biotechnology

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The economic viability of bio-production processes is often limited by damage to the microbial cell membrane and thus there is a demand for strategies to increase the robustness of the cell membrane. Damage to the microbial membrane is also a common mode of action by antibiotics. Membrane-impermeable DNA-binding dyes are often used to assess membrane integrity in conjunction with flow cytometry. We demonstrate that in situ assessment of the membrane permeability of E. coli to SYTOX Green is consistent with flow cytometry, with the benefit of lower experimental intensity, lower cost, and no need for a priori selection of sampling times. This method is demonstrated by the characterization of four membrane engineering strategies (deletion of aas, deletion of cfa, increased expression of cfa, and deletion of bhsA) for their effect on octanoic acid tolerance, with the finding that deletion of bhsA increased tolerance and substantially decreased membrane leakage. ABSTRACTThe economic viability of bio-production processes is often limited by damage to the microbial cell membrane and thus there is a demand for strategies to increase the robustness of the cell membrane. Damage to the microbial membrane is also a common mode of action by antibiotics. Membrane-impermeable DNA-binding dyes are often used to assess membrane integrity in conjunction with flow cytometry. We demonstrate that in situ assessment of the membrane permeability of E. coli to SYTOX Green is consistent with flow cytometry, with the benefit of lower experimental intensity, lower cost, and no need for a priori selection of sampling times. This method is demonstrated by the characterization of four membrane engineering strategies (deletion of aas, deletion of cfa, increased expression of cfa, and deletion of bhsA) for their effect on octanoic acid tolerance, with the finding that deletion of bhsA increased tolerance and substantially decreased membrane leakage.

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Strategies for enhancing microbial tolerance to inhibitors for biofuel production: A review

Cited by 137 publications

References 99 publications

An Overview on the Toxicological Properties of Ionic Liquids toward Microorganisms

An Overview on the Toxicological Properties of Ionic Liquids toward Microorganisms

Overcoming lignocellulose‐derived microbial inhibitors: advancing the Saccharomyces cerevisiae resistance toolbox

Streamlined assessment of membrane permeability and its application to membrane engineering of Escherichia coli for octanoic acid tolerance

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